Experiment set40IT084 for Pseudomonas putida KT2440

Compare to:

P. putida South MOI 0.1

Group: phage
Media: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: Putida_ML5, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 1/12/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 27 genes in this experiment

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 2
Peptidoglycan Biosynthesis 2
ABC transporter dipeptide (TC 3.A.1.5.2) 1
Aromatic amino acid degradation 1
Bacterial Chemotaxis 1
Conserved gene cluster associated with Met-tRNA formyltransferase 1
DNA-replication 1
Multidrug Resistance Efflux Pumps 1
Purine Utilization 1
Queuosine-Archaeosine Biosynthesis 1
Ton and Tol transport systems 1
Transcription factors bacterial 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycine betaine biosynthesis I (Gram-negative bacteria) 2 2 1
glycine betaine biosynthesis II (Gram-positive bacteria) 2 2 1
choline degradation I 2 2 1
choline-O-sulfate degradation 3 3 1