Experiment set40IT076 for Escherichia coli BW25113

Compare to:

carbon source D-Glucose 0.5mM

200 most detrimental genes:

  gene name fitness t score description  
b2904 gcvH +4.0 13.7 glycine cleavage system protein H (NCBI) compare
b2903 gcvP +3.3 26.7 glycine dehydrogenase (NCBI) compare
b2905 gcvT +2.7 16.7 glycine cleavage system aminomethyltransferase T (NCBI) compare
b4402 yjjY +1.9 2.4 hypothetical protein (NCBI) compare
b1332 ynaJ +1.8 1.6 predicted inner membrane protein (NCBI) compare
b2808 gcvA +1.8 9.1 DNA-binding transcriptional dual regulator (NCBI) compare
b4163 glyV +1.6 2.3 tRNA-Gly (NCBI) compare
b1506 yneL +1.4 1.9 predicted transcriptional regulator (NCBI) compare
b2951 yggS +1.4 5.4 predicted enzyme (NCBI) compare
b3806 cyaA +1.3 6.3 adenylate cyclase (NCBI) compare
b4701 sokX +1.3 1.2 sok-related sRNA, function unknown (RefSeq) compare
b4505 insX +1.3 2.5 predicted IS protein (RefSeq) compare
b2209 eco +1.3 2.0 ecotin precursor (NCBI) compare
b2783 chpR +1.2 2.0 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b2688 gshA +1.2 7.2 glutamate--cysteine ligase (NCBI) compare
b2642 yfjW +1.2 1.3 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0728 sucC +1.1 6.4 succinyl-CoA synthetase subunit beta (NCBI) compare
b2947 gshB +1.1 5.1 glutathione synthetase (NCBI) compare
b4203 rplI +1.1 5.2 50S ribosomal protein L9 (NCBI) compare
b4568 ytjA +1.0 2.6 predicted protein (RefSeq) compare
b2141 yohJ +1.0 2.3 hypothetical protein (NCBI) compare
b4431 rprA +1.0 2.6 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b3194 yrbE +1.0 4.8 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4647 mokA +1.0 0.8 no description compare
b4294 insA-7 +1.0 2.4 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b4503 yafF +1.0 1.3 no description compare
b4394 yjjX +0.9 2.4 orf, hypothetical protein (VIMSS) compare
b1290 sapF +0.9 1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4144 yjeI +0.9 2.3 orf, hypothetical protein (VIMSS) compare
b1724 ydiZ +0.9 1.3 hypothetical protein (NCBI) compare
b3778 rep +0.9 5.6 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b4410 ecnA +0.9 1.5 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b2635 ypjK +0.9 2.3 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2846 yqeH +0.9 0.6 orf, hypothetical protein (VIMSS) compare
b2279 nuoK +0.9 1.2 NADH dehydrogenase subunit K (NCBI) compare
b3863 polA +0.9 1.4 DNA polymerase I (NCBI) compare
b0729 sucD +0.9 3.0 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3426 glpD +0.9 3.0 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b2221 atoD +0.9 3.6 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b1620 malI +0.9 1.6 DNA-binding transcriptional repressor (NCBI) compare
b2080 b2080 +0.9 2.0 orf, hypothetical protein (VIMSS) compare
b0627 tatE +0.9 1.8 twin arginine translocase protein E (NCBI) compare
b2258 b2258 +0.8 1.2 putative transport/receptor protein (VIMSS) compare
b4552 yrhC +0.8 1.0 no description compare
b1426 ydcH +0.8 1.5 orf, hypothetical protein (VIMSS) compare
b2817 b2817 +0.8 3.9 putative amidase (VIMSS) compare
b1150 ymfR +0.8 1.0 e14 prophage; predicted protein (NCBI) compare
b1273 yciN +0.8 1.7 hypothetical protein (NCBI) compare
b1412 azoR +0.8 1.5 acyl carrier protein phosphodiesterase (NCBI) compare
b0688 pgm +0.8 2.8 phosphoglucomutase (NCBI) compare
b1360 ydaV +0.8 1.5 Rac prophage; predicted DNA replication protein (NCBI) compare
b1524 yneH +0.8 0.8 predicted glutaminase (NCBI) compare
b1667 ydhR +0.7 1.5 hypothetical protein (NCBI) compare
b1493 gadB +0.7 0.8 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1686 ydiI +0.7 1.4 hypothetical protein (NCBI) compare
b1824 yobF +0.7 1.7 hypothetical protein (NCBI) compare
b4641 yoeH +0.7 2.6 no description compare
b2910 zapA +0.7 2.8 protein that localizes to the cytokinetic ring (NCBI) compare
b4435 isrC +0.7 1.4 unknown RNA (NCBI) compare
b0998 torD +0.7 0.9 chaperone protein TorD (NCBI) compare
b2948 yqgE +0.7 1.2 orf, hypothetical protein (VIMSS) compare
b4012 yjaB +0.7 2.0 predicted acetyltransferase (NCBI) compare
b1095 fabF +0.7 4.3 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1939 fliG +0.7 3.1 flagellar motor switch protein G (NCBI) compare
b4656 yjhY +0.7 2.0 no description compare
b3842 rfaH +0.7 3.4 transcriptional activator RfaH (NCBI) compare
b1006 ycdG +0.7 3.5 putative transport protein (VIMSS) compare
b3005 exbD +0.7 3.2 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1434 ydcN +0.7 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2823 ppdC +0.7 3.4 hypothetical protein (NCBI) compare
b4592 yccB +0.7 1.2 hypothetical protein (RefSeq) compare
b4260 pepA +0.6 4.3 leucyl aminopeptidase (NCBI) compare
b4407 thiS +0.6 1.7 sulfur carrier protein ThiS (NCBI) compare
b3119 tdcR +0.6 1.4 threonine dehydratase operon activator protein (VIMSS) compare
b4702 mgtL +0.6 1.0 regulatory leader peptide for mgtA (NCBI) compare
b1841 yobA +0.6 1.7 hypothetical protein (NCBI) compare
b1636 pdxY +0.6 2.8 pyridoxine kinase (NCBI) compare
b1482 osmC +0.6 1.2 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b2450 yffS +0.6 2.0 CPZ-55 prophage; predicted protein (RefSeq) compare
b2480 bcp +0.6 1.9 thioredoxin-dependent thiol peroxidase (NCBI) compare
b1630 rsxD +0.6 2.6 electron transport complex protein RnfD (NCBI) compare
b1758 b1758 +0.6 1.0 putative cytochrome oxidase (VIMSS) compare
b2437 yfeG +0.6 3.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1335 ogt +0.6 2.1 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b1235 rssB +0.6 2.6 response regulator of RpoS (NCBI) compare
b1441 ydcT +0.6 2.1 predicted spermidine/putrescine transporter subunit (NCBI) compare
b0323 yahI +0.6 3.6 predicted carbamate kinase-like protein (NCBI) compare
b2488 hyfH +0.6 2.4 hydrogenase 4, Fe-S subunit (NCBI) compare
b1920 fliY +0.6 1.7 cystine transporter subunit (NCBI) compare
b1660 ydhC +0.6 2.6 predicted transporter (NCBI) compare
b4373 rimI +0.6 1.5 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1236 galU +0.6 3.0 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3163 nlpI +0.6 1.8 hypothetical protein (NCBI) compare
b4653 yicT +0.6 0.9 no description compare
b2830 nudH +0.6 0.7 dinucleoside polyphosphate hydrolase (NCBI) compare
b0339 cynT +0.6 2.3 carbonic anhydrase (NCBI) compare
b2064 asmA +0.6 3.2 predicted assembly protein (NCBI) compare
b1925 fliS +0.6 2.4 flagellar protein FliS (NCBI) compare
b1479 sfcA +0.6 2.2 NAD-linked malate dehydrogenase (malic enzyme) (VIMSS) compare
b0059 hepA +0.6 4.7 ATP-dependent helicase HepA (NCBI) compare
b1752 ydjZ +0.6 1.7 conserved inner membrane protein (NCBI) compare
b1849 purT +0.6 2.4 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b1668 ydhS +0.6 2.2 conserved protein with FAD/NAD(P)-binding domain (NCBI) compare
b2301 yfcF +0.6 2.1 predicted enzyme (NCBI) compare
b4174 hflK +0.6 3.9 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3836 b3836 +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b3929 rraA +0.5 3.9 ribonuclease activity regulator protein RraA (NCBI) compare
b4097 phnK +0.5 1.9 carbon-phosphorus lyase complex subunit (NCBI) compare
b3677 yidI +0.5 3.8 predicted inner membrane protein (NCBI) compare
b3632 rfaQ +0.5 4.6 lipopolysaccharide core biosynthesis protein (NCBI) compare
b4232 fbp +0.5 3.6 fructose-1,6-bisphosphatase (NCBI) compare
b2829 ptsP +0.5 4.2 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2421 cysM +0.5 2.2 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b4622 ytcA +0.5 1.8 hypothetical protein (RefSeq) compare
b1082 flgK +0.5 2.5 flagellar hook-associated protein K (NCBI) compare
b2453 eutG +0.5 3.0 ethanolamine utilization; homolog of Salmonella enzyme, similar to iron-containing alcohol dehydrogenase (VIMSS) compare
b0424 thiJ +0.5 2.6 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis (VIMSS) compare
b1868 yecE +0.5 1.6 hypothetical protein (NCBI) compare
b0549 ybcO +0.5 1.2 DLP12 prophage; predicted protein (NCBI) compare
b3149 yraO +0.5 2.3 DnaA initiator-associating factor for replication initiation (NCBI) compare
b4692 oweE +0.5 1.5 no description compare
b2572 rseA +0.5 1.8 anti-sigma factor (NCBI) compare
b2811 ygdK +0.5 1.7 predicted Fe-S metabolism protein (NCBI) compare
b0580 ybdJ +0.5 0.6 predicted inner membrane protein (NCBI) compare
b1948 fliP +0.5 2.6 flagellar biosynthesis protein P (NCBI) compare
b4104 phnE +0.5 1.8 phosphonate/organophosphate ester transporter subunit (NCBI) compare
b3181 greA +0.5 2.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b4544 yfbW +0.5 1.4 hypothetical protein (NCBI) compare
b2419 yfeK +0.5 1.2 hypothetical protein (NCBI) compare
b1606 folM +0.5 3.2 short chain dehydrogenase (NCBI) compare
b4679 yohP +0.5 1.3 expressed protein (RefSeq) compare
b0296 ykgM +0.5 1.5 50S ribosomal protein L31 (NCBI) compare
b1155 ymfS +0.5 2.1 e14 prophage; predicted protein (NCBI) compare
b4536 yobH +0.5 1.7 hypothetical protein (NCBI) compare
b4416 rybA +0.5 0.9 unknown RNA (NCBI) compare
b3148 yraN +0.5 1.9 hypothetical protein (NCBI) compare
b2073 yegL +0.5 2.5 hypothetical protein (NCBI) compare
b1854 pykA +0.5 3.3 pyruvate kinase (NCBI) compare
b0490 ybbL +0.5 2.6 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1370 insH-5 +0.5 1.3 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b4093 phnO +0.5 2.1 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1952 dsrB +0.5 2.2 hypothetical protein (NCBI) compare
b1446 ydcY +0.5 1.0 hypothetical protein (NCBI) compare
b2659 b2659 +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b1110 ycfJ +0.5 2.2 hypothetical protein (NCBI) compare
b1830 prc +0.5 2.4 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2384 ypdE +0.5 2.8 predicted peptidase (NCBI) compare
b2134 pbpG +0.5 2.3 penicillin-binding protein 7 (VIMSS) compare
b1953 b1953 +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b1884 cheR +0.5 3.0 chemotaxis regulator, protein-glutamate methyltransferase (NCBI) compare
b3617 kbl +0.5 2.6 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b2104 thiM +0.5 1.1 hydroxyethylthiazole kinase (NCBI) compare
b3351 kefG +0.5 2.9 glutathione-regulated potassium-efflux system ancillary protein (NCBI) compare
b2504 yfgG +0.5 0.9 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b2637 yfjT +0.5 1.1 CP4-57 prophage; predicted protein (NCBI) compare
b1923 fliC +0.5 3.0 flagellin (NCBI) compare
b2009 sbmC +0.5 2.1 DNA gyrase inhibitor (NCBI) compare
b0496 ybbP +0.5 2.9 predicted inner membrane protein (NCBI) compare
b1125 potB +0.5 1.4 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1205 ychH +0.5 1.2 predicted inner membrane protein (NCBI) compare
b3198 kdsC +0.5 1.2 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2125 yehT +0.5 2.1 orf, hypothetical protein (VIMSS) compare
b2168 fruK +0.5 0.7 1-phosphofructokinase (NCBI) compare
b0268 yagE +0.4 1.9 2-keto-3-deoxy gluconate (KDG) aldolase; CP4-6 prophage (NCBI) compare
b0483 ybaQ +0.4 3.1 orf, hypothetical protein (VIMSS) compare
b1806 yeaY +0.4 1.8 predicted lipoprotein (NCBI) compare
b3206 npr +0.4 2.4 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2645 yfjZ +0.4 1.8 CP4-57 prophage; antitoxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b1818 manY +0.4 0.9 mannose-specific enzyme IIC component of PTS (NCBI) compare
b1817 manX +0.4 1.9 fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI) compare
b1792 yeaO +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b2079 baeR +0.4 1.6 DNA-binding response regulator in two-component regulatory system with BaeS (NCBI) compare
b1802 yeaW +0.4 2.1 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b2101 yegW +0.4 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b0356 frmA +0.4 2.3 alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI) compare
b4532 yncN +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b2699 recA +0.4 0.7 recombinase A (NCBI) compare
b2899 yqfA +0.4 2.0 predicted oxidoreductase, inner membrane subunit (NCBI) compare
b4450 arcZ +0.4 1.0 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b2883 guaD +0.4 1.9 guanine deaminase (NCBI) compare
b0927 ycbL +0.4 1.5 predicted metal-binding enzyme (NCBI) compare
b2066 udk +0.4 1.7 uridine/cytidine kinase (VIMSS) compare
b2393 nupC +0.4 2.2 nucleoside (except guanosine) transporter (NCBI) compare
b2704 srlB +0.4 0.9 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b0548 ninE +0.4 1.2 DLP12 prophage; conserved protein (NCBI) compare
b4385 yjjJ +0.4 2.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1961 dcm +0.4 0.9 DNA cytosine methylase (NCBI) compare
b2317 dedA +0.4 1.5 conserved inner membrane protein (NCBI) compare
b0979 appB +0.4 2.0 cytochrome bd-II oxidase, subunit II (NCBI) compare
b0830 yliB +0.4 2.7 predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b3404 envZ +0.4 2.6 osmolarity sensor protein (NCBI) compare
b1308 pspE +0.4 2.2 thiosulfate:cyanide sulfurtransferase (rhodanese) (NCBI) compare
b1064 grxB +0.4 2.6 glutaredoxin 2 (Grx2) (NCBI) compare
b1468 narZ +0.4 2.6 nitrate reductase 2 (NRZ), alpha subunit (NCBI) compare
b0480 ushA +0.4 2.1 UDP-sugar hydrolase (NCBI) compare
b4603 rseX +0.4 0.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b3188 sfsB +0.4 1.8 DNA-binding transcriptional activator of maltose metabolism (NCBI) compare
b2550 yphH +0.4 1.8 putative NAGC-like transcriptional regulator (VIMSS) compare
b0966 hspQ +0.4 1.8 DNA-binding protein, hemimethylated (RefSeq) compare
b1989 asnV +0.4 1.1 tRNA-Asn (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in carbon source experiments

For carbon source D-Glucose across organisms