Experiment set40IT067 for Escherichia coli BW25113

Compare to:

carbon source D-Mannose 20mM

200 most detrimental genes:

  gene name fitness t score description  
b2904 gcvH +4.9 16.5 glycine cleavage system protein H (NCBI) compare
b2903 gcvP +4.0 36.1 glycine dehydrogenase (NCBI) compare
b2951 yggS +3.7 20.4 predicted enzyme (NCBI) compare
b1830 prc +3.6 19.1 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2905 gcvT +3.5 20.3 glycine cleavage system aminomethyltransferase T (NCBI) compare
b2572 rseA +3.4 6.1 anti-sigma factor (NCBI) compare
b3163 nlpI +3.4 15.1 hypothetical protein (NCBI) compare
b3784 rfe +2.8 24.5 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3405 ompR +2.8 13.2 osmolarity response regulator (NCBI) compare
b2829 ptsP +2.8 20.6 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3620 rfaF +2.7 18.6 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3911 cpxA +2.7 17.4 two-component sensor protein (NCBI) compare
b3621 rfaC +2.7 15.4 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0222 lpcA +2.6 7.0 phosphoheptose isomerase (NCBI) compare
b3052 rfaE +2.6 11.8 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3787 rffD +2.6 21.5 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1677 lpp +2.5 9.0 murein lipoprotein (NCBI) compare
b3786 rffE +2.5 21.0 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3472 b3472 +2.4 21.0 orf, hypothetical protein (VIMSS) compare
b3794 rffM +2.4 17.7 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b2017 yefM +2.3 2.8 orf, hypothetical protein (VIMSS) compare
b3206 npr +2.3 14.2 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b0053 surA +2.3 13.5 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0178 hlpA +2.3 12.7 periplasmic chaperone (NCBI) compare
b2808 gcvA +2.3 7.9 DNA-binding transcriptional dual regulator (NCBI) compare
b3082 ygjM +2.3 4.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b4042 dgkA +2.2 6.0 diacylglycerol kinase (NCBI) compare
b3021 ygiT +2.1 6.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b3619 rfaD +2.1 10.3 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3341 rpsG +2.1 4.7 30S ribosomal protein S7 (NCBI) compare
b3529 yhjK +2.0 15.3 predicted diguanylate cyclase (RefSeq) compare
b3655 yicH +2.0 17.0 hypothetical protein (NCBI) compare
b3164 pnp +2.0 5.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1575 dicB +1.9 4.7 Qin prophage; cell division inhibition protein (NCBI) compare
b4203 rplI +1.8 8.5 50S ribosomal protein L9 (NCBI) compare
b3791 rffA +1.7 10.7 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0232 yafN +1.7 2.2 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3785 wzzE +1.7 11.2 putative transport protein (VIMSS) compare
b0059 hepA +1.7 14.5 ATP-dependent helicase HepA (NCBI) compare
b3842 rfaH +1.7 6.5 transcriptional activator RfaH (NCBI) compare
b4481 rffT +1.6 9.1 4-alpha-L-fucosyltransferase (NCBI) compare
b2144 sanA +1.6 11.7 hypothetical protein (NCBI) compare
b3630 rfaP +1.6 12.6 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0092 ddlB +1.6 11.1 D-alanylalanine synthetase (NCBI) compare
b3199 yrbK +1.6 3.9 hypothetical protein (NCBI) compare
b3753 rbsR +1.6 12.0 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b4702 mgtL +1.6 1.7 regulatory leader peptide for mgtA (NCBI) compare
b0957 ompA +1.5 6.6 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b0437 clpP +1.5 6.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3643 rph +1.5 4.8 ribonuclease PH (NCBI) compare
b3790 rffC +1.5 6.4 TDP-fucosamine acetyltransferase (RefSeq) compare
b1667 ydhR +1.5 3.5 hypothetical protein (NCBI) compare
b1709 btuD +1.5 1.6 vitamin B12-transporter ATPase (NCBI) compare
b3631 rfaG +1.5 12.2 glucosyltransferase I (NCBI) compare
b3261 fis +1.4 4.6 DNA-binding protein Fis (NCBI) compare
b0632 dacA +1.4 7.7 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b2894 xerD +1.4 1.8 site-specific tyrosine recombinase XerD (NCBI) compare
b4202 rpsR +1.4 2.1 30S ribosomal protein S18 (NCBI) compare
b2830 nudH +1.4 2.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b4410 ecnA +1.4 2.1 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b2912 ygfA +1.3 3.1 putative ligase (VIMSS) compare
b3977 tyrU +1.3 1.6 tRNA-Tyr (NCBI) compare
b1095 fabF +1.3 7.1 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b2742 nlpD +1.3 7.4 predicted outer membrane lipoprotein (NCBI) compare
b3229 sspA +1.3 3.3 stringent starvation protein A (NCBI) compare
b0858 ybjO +1.3 2.7 predicted inner membrane protein (NCBI) compare
b0627 tatE +1.3 3.4 twin arginine translocase protein E (NCBI) compare
b2688 gshA +1.3 6.2 glutamate--cysteine ligase (NCBI) compare
b2494 yfgC +1.2 6.4 predicted peptidase (NCBI) compare
b3627 rfaI +1.2 7.5 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b0438 clpX +1.2 5.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3181 greA +1.1 7.7 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b4535 yniD +1.1 1.2 predicted protein (RefSeq) compare
b4169 amiB +1.1 7.7 N-acetylmuramoyl-l-alanine amidase II (NCBI) compare
b0998 torD +1.1 1.3 chaperone protein TorD (NCBI) compare
b1630 rsxD +1.1 3.8 electron transport complex protein RnfD (NCBI) compare
b2947 gshB +1.1 3.7 glutathione synthetase (NCBI) compare
b2516 yfgA +1.1 5.4 hypothetical protein (NCBI) compare
b0688 pgm +1.1 3.3 phosphoglucomutase (NCBI) compare
b4172 hfq +1.1 1.8 RNA-binding protein Hfq (NCBI) compare
b4592 yccB +1.1 2.6 hypothetical protein (RefSeq) compare
b3150 yraP +1.1 5.2 hypothetical protein (NCBI) compare
b3404 envZ +1.1 6.4 osmolarity sensor protein (NCBI) compare
b1824 yobF +1.1 2.5 hypothetical protein (NCBI) compare
b4609 ryfD +1.1 2.8 Novel sRNA, function unknown (RefSeq) compare
b2258 b2258 +1.0 1.3 putative transport/receptor protein (VIMSS) compare
b2614 grpE +1.0 1.5 heat shock protein (NCBI) compare
b3778 rep +1.0 5.8 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1855 lpxM +1.0 3.4 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4000 hupA +1.0 3.3 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0791 ybhQ +1.0 3.0 predicted inner membrane protein (NCBI) compare
b2635 ypjK +1.0 3.2 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0683 fur +1.0 0.9 ferric uptake regulator (NCBI) compare
b2512 yfgL +1.0 5.1 protein assembly complex, lipoprotein component (NCBI) compare
b1609 rstB +1.0 3.5 sensory histidine kinase in two-component regulatory system with RstA (NCBI) compare
b4505 insX +1.0 1.0 predicted IS protein (RefSeq) compare
b1190 dadX +1.0 4.4 alanine racemase (NCBI) compare
b4438 cyaR +0.9 1.8 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1446 ydcY +0.9 2.1 hypothetical protein (NCBI) compare
b1222 narX +0.9 4.0 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b0852 rimK +0.9 5.6 ribosomal protein S6 modification protein (NCBI) compare
b2141 yohJ +0.9 1.5 hypothetical protein (NCBI) compare
b4174 hflK +0.9 5.5 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1434 ydcN +0.9 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1573 ydfC +0.9 1.4 Qin prophage; predicted protein (RefSeq) compare
b4012 yjaB +0.9 2.8 predicted acetyltransferase (NCBI) compare
b3979 thrT +0.9 0.4 tRNA-Thr (NCBI) compare
b1150 ymfR +0.9 0.9 e14 prophage; predicted protein (NCBI) compare
b1685 ydiH +0.9 1.5 predicted protein (RefSeq) compare
b0695 kdpD +0.9 3.5 fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein (NCBI) compare
b4135 yjdC +0.9 4.8 orf, hypothetical protein (VIMSS) compare
b4375 prfC +0.8 3.1 peptide chain release factor 3 (NCBI) compare
b1013 ycdC +0.8 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1271 yciK +0.8 1.8 short chain dehydrogenase (NCBI) compare
b3632 rfaQ +0.8 6.8 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2480 bcp +0.8 3.5 thioredoxin-dependent thiol peroxidase (NCBI) compare
b0226 dinJ +0.8 2.6 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b0953 rmf +0.8 1.4 ribosome modulation factor (NCBI) compare
b1891 flhC +0.8 2.4 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b2291 yfbR +0.8 3.2 hypothetical protein (NCBI) compare
b1400 paaY +0.8 1.1 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b4294 insA-7 +0.8 1.7 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b3789 rffH +0.8 4.8 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b2317 dedA +0.8 2.7 conserved inner membrane protein (NCBI) compare
b3806 cyaA +0.8 3.4 adenylate cyclase (NCBI) compare
b1412 azoR +0.8 1.8 acyl carrier protein phosphodiesterase (NCBI) compare
b4450 arcZ +0.8 2.3 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b2817 b2817 +0.8 3.7 putative amidase (VIMSS) compare
b4163 glyV +0.8 1.2 tRNA-Gly (NCBI) compare
b1448 yncA +0.8 1.8 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1294 sapA +0.8 4.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1651 gloA +0.8 1.8 glyoxalase I, Ni-dependent (NCBI) compare
b1299 puuR +0.8 1.8 DNA-binding transcriptional repressor (NCBI) compare
b0082 mraW +0.8 2.3 S-adenosyl-methyltransferase (NCBI) compare
b1524 yneH +0.8 0.7 predicted glutaminase (NCBI) compare
b2209 eco +0.8 1.0 ecotin precursor (NCBI) compare
b4373 rimI +0.8 2.1 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b0728 sucC +0.8 3.5 succinyl-CoA synthetase subunit beta (NCBI) compare
b0866 ybjQ +0.8 0.9 hypothetical protein (NCBI) compare
b0882 clpA +0.7 4.7 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b2259 pmrD +0.7 0.8 polymyxin resistance protein B (VIMSS) compare
b1348 lar +0.7 1.8 Rac prophage; restriction alleviation protein (NCBI) compare
b3102 yqjG +0.7 2.3 predicted S-transferase (NCBI) compare
b3198 kdsC +0.7 1.8 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2455 eutE +0.7 2.2 predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI) compare
b0423 thiI +0.7 3.1 thiamine biosynthesis protein ThiI (NCBI) compare
b1926 fliT +0.7 2.7 predicted chaperone (NCBI) compare
b2352 yfdI +0.7 1.0 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b2743 pcm +0.7 2.6 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b4413 sokC +0.7 1.1 regulatory antisense RNA (NCBI) compare
b1640 ydhH +0.7 3.4 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b3388 damX +0.7 5.6 hypothetical protein (NCBI) compare
b4221 ytfN +0.7 6.0 hypothetical protein (NCBI) compare
b0686 ybfF +0.7 3.7 hypothetical protein (NCBI) compare
b1441 ydcT +0.7 2.2 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1841 yobA +0.7 1.2 hypothetical protein (NCBI) compare
b2962 yggX +0.7 2.3 hypothetical protein (NCBI) compare
b1426 ydcH +0.7 1.1 orf, hypothetical protein (VIMSS) compare
b0405 queA +0.7 3.7 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI) compare
b2659 b2659 +0.7 1.6 orf, hypothetical protein (VIMSS) compare
b0502 ylbG +0.7 0.8 no description compare
b1507 hipA +0.7 1.9 regulator with hipB (NCBI) compare
b3351 kefG +0.7 3.9 glutathione-regulated potassium-efflux system ancillary protein (NCBI) compare
b0853 ybjN +0.7 1.5 predicted oxidoreductase (NCBI) compare
b0193 yaeF +0.7 3.3 orf, hypothetical protein (VIMSS) compare
b1415 aldA +0.7 2.3 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b0729 sucD +0.7 1.7 succinyl-CoA synthetase subunit alpha (NCBI) compare
b0949 uup +0.7 3.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3761 trpT +0.7 1.0 tRNA-Trp (NCBI) compare
b4522 yciX_1 +0.7 1.5 hypothetical protein (NCBI) compare
b1421 trg +0.7 3.0 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b3387 dam +0.7 4.6 DNA adenine methylase (NCBI) compare
b3929 rraA +0.7 3.3 ribonuclease activity regulator protein RraA (NCBI) compare
b1300 puuC +0.7 2.0 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) compare
b2052 fcl +0.7 2.6 bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI) compare
b2066 udk +0.7 1.8 uridine/cytidine kinase (VIMSS) compare
b2711 norW +0.7 3.1 nitric oxide reductase (NCBI) compare
b2992 hybE +0.7 2.8 hydrogenase 2-specific chaperone (NCBI) compare
b1514 lsrC +0.6 1.1 AI2 transporter (NCBI) compare
b1556 b1556 +0.6 1.6 orf, hypothetical protein (VIMSS) compare
b3035 tolC +0.6 3.3 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1301 puuB +0.6 2.4 gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI) compare
b2539 hcaF +0.6 2.7 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI) compare
b0296 ykgM +0.6 1.7 50S ribosomal protein L31 (NCBI) compare
b2297 pta +0.6 0.4 phosphate acetyltransferase (NCBI) compare
b3869 glnL +0.6 3.4 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) compare
b4646 yrdE +0.6 2.2 no description compare
b4220 ytfM +0.6 4.6 predicted outer membrane protein and surface antigen (NCBI) compare
b1074 flgC +0.6 1.5 flagellar basal-body rod protein C (NCBI) compare
b4293 fecI +0.6 2.2 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b1129 phoQ +0.6 1.3 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b1884 cheR +0.6 2.8 chemotaxis regulator, protein-glutamate methyltransferase (NCBI) compare
b2457 cchA +0.6 1.6 detox protein (VIMSS) compare
b1632 rsxE +0.6 3.0 NADH-ubiquinone oxidoreductase (NCBI) compare
b1629 rsxC +0.6 3.0 electron transport complex protein RnfC (NCBI) compare
b1757 b1757 +0.6 2.3 putative thiosulfate sulfur transferase (VIMSS) compare
b0249 ykfF +0.6 1.1 CP4-6 prophage; predicted protein (NCBI) compare
b3494 yhiO +0.6 4.2 universal stress protein UspB (NCBI) compare
b1650 nemA +0.6 1.6 N-ethylmaleimide reductase, FMN-linked (NCBI) compare
b4141 yjeH +0.6 3.9 predicted transporter (NCBI) compare


Specific Phenotypes

For 6 genes in this experiment

For carbon source D-Mannose in Escherichia coli BW25113

For carbon source D-Mannose across organisms