Experiment set40IT065 for Escherichia coli BW25113

Compare to:

carbon source D-Glucose 20mM

200 most detrimental genes:

  gene name fitness t score description  
b2904 gcvH +4.3 13.0 glycine cleavage system protein H (NCBI) compare
b3035 tolC +4.2 28.2 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b2829 ptsP +3.8 25.1 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3206 npr +3.7 18.5 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2903 gcvP +3.6 31.4 glycine dehydrogenase (NCBI) compare
b2144 sanA +3.5 27.1 hypothetical protein (NCBI) compare
b2905 gcvT +3.3 21.0 glycine cleavage system aminomethyltransferase T (NCBI) compare
b0632 dacA +3.3 18.7 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3405 ompR +3.2 12.6 osmolarity response regulator (NCBI) compare
b1830 prc +3.1 16.6 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3082 ygjM +3.1 4.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3911 cpxA +3.0 21.5 two-component sensor protein (NCBI) compare
b0437 clpP +2.9 12.6 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1677 lpp +2.9 14.8 murein lipoprotein (NCBI) compare
b3784 rfe +2.8 22.9 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3787 rffD +2.7 21.0 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b0438 clpX +2.7 14.6 ATP-dependent protease ATP-binding subunit (NCBI) compare
b2572 rseA +2.7 4.9 anti-sigma factor (NCBI) compare
b3199 yrbK +2.6 10.1 hypothetical protein (NCBI) compare
b3404 envZ +2.6 15.8 osmolarity sensor protein (NCBI) compare
b2808 gcvA +2.6 9.6 DNA-binding transcriptional dual regulator (NCBI) compare
b2188 yejM +2.5 14.5 predicted hydrolase, inner membrane (NCBI) compare
b3786 rffE +2.5 20.5 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2951 yggS +2.4 11.9 predicted enzyme (NCBI) compare
b3794 rffM +2.4 18.2 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b0178 hlpA +2.4 10.5 periplasmic chaperone (NCBI) compare
b4042 dgkA +2.3 5.2 diacylglycerol kinase (NCBI) compare
b2064 asmA +2.3 8.3 predicted assembly protein (NCBI) compare
b1332 ynaJ +2.2 1.7 predicted inner membrane protein (NCBI) compare
b2830 nudH +2.1 4.3 dinucleoside polyphosphate hydrolase (NCBI) compare
b2017 yefM +2.1 2.8 orf, hypothetical protein (VIMSS) compare
b3021 ygiT +2.1 3.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b3261 fis +2.1 8.0 DNA-binding protein Fis (NCBI) compare
b3164 pnp +2.0 6.0 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b2742 nlpD +2.0 11.1 predicted outer membrane lipoprotein (NCBI) compare
b4402 yjjY +1.9 1.8 hypothetical protein (NCBI) compare
b4481 rffT +1.9 10.3 4-alpha-L-fucosyltransferase (NCBI) compare
b4169 amiB +1.8 12.7 N-acetylmuramoyl-l-alanine amidase II (NCBI) compare
b1607 ydgC +1.8 2.2 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b4505 insX +1.8 2.2 predicted IS protein (RefSeq) compare
b3621 rfaC +1.8 8.4 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0092 ddlB +1.8 6.0 D-alanylalanine synthetase (NCBI) compare
b3791 rffA +1.7 10.6 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3790 rffC +1.7 7.6 TDP-fucosamine acetyltransferase (RefSeq) compare
b4592 yccB +1.7 1.6 hypothetical protein (RefSeq) compare
b4000 hupA +1.7 7.9 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3341 rpsG +1.7 2.4 30S ribosomal protein S7 (NCBI) compare
b3052 rfaE +1.7 7.8 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3163 nlpI +1.7 5.8 hypothetical protein (NCBI) compare
b3529 yhjK +1.6 11.8 predicted diguanylate cyclase (RefSeq) compare
b0677 nagA +1.6 7.1 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b4426 isrA +1.6 3.1 unknown RNA (NCBI) compare
b0059 hepA +1.6 12.4 ATP-dependent helicase HepA (NCBI) compare
b3387 dam +1.6 11.5 DNA adenine methylase (NCBI) compare
b0695 kdpD +1.6 5.6 fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein (NCBI) compare
b0222 lpcA +1.6 3.9 phosphoheptose isomerase (NCBI) compare
b4203 rplI +1.5 4.9 50S ribosomal protein L9 (NCBI) compare
b1273 yciN +1.5 2.7 hypothetical protein (NCBI) compare
b3620 rfaF +1.5 8.2 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0053 surA +1.5 8.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1293 sapB +1.4 2.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3107 yhaL +1.4 2.3 orf, hypothetical protein (VIMSS) compare
b1709 btuD +1.4 1.3 vitamin B12-transporter ATPase (NCBI) compare
b3150 yraP +1.4 6.7 hypothetical protein (NCBI) compare
b3655 yicH +1.4 11.5 hypothetical protein (NCBI) compare
b2516 yfgA +1.4 7.0 hypothetical protein (NCBI) compare
b3778 rep +1.4 6.3 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1754 b1754 +1.3 3.0 orf, hypothetical protein (VIMSS) compare
b1291 sapD +1.3 1.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3619 rfaD +1.3 6.1 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b4593 ymgI +1.3 1.4 hypothetical protein (RefSeq) compare
b3443 yrhA +1.3 1.8 orf, hypothetical protein (VIMSS) compare
b3472 b3472 +1.3 9.1 orf, hypothetical protein (VIMSS) compare
b3920 yiiQ +1.3 7.5 hypothetical protein (NCBI) compare
b4545 ypdJ +1.3 1.9 no description compare
b2688 gshA +1.3 6.7 glutamate--cysteine ligase (NCBI) compare
b3785 wzzE +1.2 8.5 putative transport protein (VIMSS) compare
b2175 spr +1.2 3.2 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3630 rfaP +1.2 8.4 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0866 ybjQ +1.2 1.5 hypothetical protein (NCBI) compare
b3631 rfaG +1.2 9.2 glucosyltransferase I (NCBI) compare
b0990 cspG +1.1 1.1 DNA-binding transcriptional regulator (NCBI) compare
b2362 yfdS +1.1 3.4 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b3935 priA +1.1 0.9 primosome assembly protein PriA (NCBI) compare
b1818 manY +1.1 3.1 mannose-specific enzyme IIC component of PTS (NCBI) compare
b0502 ylbG +1.1 1.5 no description compare
b2426 ucpA +1.1 1.1 putative oxidoreductase (VIMSS) compare
b3842 rfaH +1.1 4.7 transcriptional activator RfaH (NCBI) compare
b1630 rsxD +1.1 3.7 electron transport complex protein RnfD (NCBI) compare
b3260 dusB +1.1 4.4 tRNA-dihydrouridine synthase B (NCBI) compare
b1380 ldhA +1.0 2.5 D-lactate dehydrogenase (NCBI) compare
b2817 b2817 +1.0 4.1 putative amidase (VIMSS) compare
b1861 ruvA +1.0 1.2 Holliday junction DNA helicase motor protein (NCBI) compare
b1824 yobF +1.0 4.4 hypothetical protein (NCBI) compare
b1741 ydjQ +1.0 2.4 endonuclease of nucleotide excision repair (NCBI) compare
b1575 dicB +1.0 1.4 Qin prophage; cell division inhibition protein (NCBI) compare
b3652 recG +1.0 6.0 ATP-dependent DNA helicase (NCBI) compare
b1855 lpxM +1.0 3.1 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3102 yqjG +1.0 2.3 predicted S-transferase (NCBI) compare
b3806 cyaA +1.0 3.9 adenylate cyclase (NCBI) compare
b2540 hcaC +1.0 2.4 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI) compare
b2313 cvpA +1.0 1.3 membrane protein required for colicin V production (NCBI) compare
b3229 sspA +0.9 2.8 stringent starvation protein A (NCBI) compare
b1085 b1085 +0.9 2.1 orf, hypothetical protein (VIMSS) compare
b2937 speB +0.9 4.3 agmatinase (NCBI) compare
b3616 tdh +0.9 4.3 L-threonine 3-dehydrogenase (NCBI) compare
b4155 yjeA +0.9 1.3 putative lysyl-tRNA synthetase (VIMSS) compare
b3609 secB +0.9 1.9 export protein SecB (NCBI) compare
b0082 mraW +0.9 2.1 S-adenosyl-methyltransferase (NCBI) compare
b0882 clpA +0.9 5.4 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b1095 fabF +0.9 4.7 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1863 ruvC +0.9 2.4 Holliday junction resolvase (NCBI) compare
b2498 upp +0.9 1.8 uracil phosphoribosyltransferase (VIMSS) compare
b2110 yehC +0.9 1.6 predicted periplasmic pilin chaperone (NCBI) compare
b1186 nhaB +0.9 4.6 NhaB sodium/proton transporter (Katherine Huang) compare
b0605 ahpC +0.9 2.7 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b2445 yffN +0.9 2.8 CPZ-55 prophage; predicted protein (RefSeq) compare
b2768 ygcP +0.9 1.8 predicted anti-terminator regulatory protein (NCBI) compare
b2444 yffM +0.9 2.1 CPZ-55 prophage; predicted protein (NCBI) compare
b3977 tyrU +0.9 0.9 tRNA-Tyr (NCBI) compare
b2947 gshB +0.9 3.3 glutathione synthetase (NCBI) compare
b1159 mcrA +0.9 1.3 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b2221 atoD +0.9 1.5 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b4410 ecnA +0.8 1.0 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1438 ydcQ +0.8 2.1 predicted DNA-binding transcriptional regulator (RefSeq) compare
b2212 alkB +0.8 1.8 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b2297 pta +0.8 0.5 phosphate acetyltransferase (NCBI) compare
b2953 yggU +0.8 2.0 orf, hypothetical protein (VIMSS) compare
b3625 rfaY +0.8 3.0 lipopolysaccharide core biosynthesis protein (NCBI) compare
b3181 greA +0.8 4.2 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1478 adhP +0.8 1.6 alcohol dehydrogenase (VIMSS) compare
b1542 ydfI +0.8 2.1 predicted mannonate dehydrogenase (NCBI) compare
b4535 yniD +0.8 0.6 predicted protein (RefSeq) compare
b1524 yneH +0.8 0.7 predicted glutaminase (NCBI) compare
b1685 ydiH +0.8 1.2 predicted protein (RefSeq) compare
b1236 galU +0.8 2.9 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1290 sapF +0.8 1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4543 ypaA +0.8 2.2 predicted protein (NCBI) compare
b4163 glyV +0.8 1.0 tRNA-Gly (NCBI) compare
b0929 ompF +0.8 3.0 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b4441 glmY +0.8 1.3 sRNA activator of glmS mRNA (RefSeq) compare
b2007 yeeX +0.8 2.9 conserved protein (RefSeq) compare
b2635 ypjK +0.8 1.3 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1271 yciK +0.8 1.7 short chain dehydrogenase (NCBI) compare
b0759 galE +0.8 3.5 UDP-galactose-4-epimerase (VIMSS) compare
b2446 yffO +0.8 1.2 CPZ-55 prophage; predicted protein (NCBI) compare
b3149 yraO +0.8 3.0 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1111 ycfQ +0.8 2.2 orf, hypothetical protein (VIMSS) compare
b0296 ykgM +0.8 1.4 50S ribosomal protein L31 (NCBI) compare
b1984 asnW +0.8 0.9 tRNA-Asn (NCBI) compare
b4676 yoaK +0.7 0.4 expressed protein (RefSeq) compare
b3469 zntA +0.7 3.7 zinc, cobalt and lead efflux system (NCBI) compare
b3426 glpD +0.7 2.4 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b2356 yfdM +0.7 1.5 orf, hypothetical protein (VIMSS) compare
b0729 sucD +0.7 1.7 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1948 fliP +0.7 2.0 flagellar biosynthesis protein P (NCBI) compare
b4294 insA-7 +0.7 0.6 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b4595 yciY +0.7 1.7 hypothetical protein (RefSeq) compare
b2480 bcp +0.7 2.1 thioredoxin-dependent thiol peroxidase (NCBI) compare
b2684 mprA +0.7 2.1 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1097 yceG +0.7 3.9 predicted aminodeoxychorismate lyase (NCBI) compare
b3643 rph +0.7 2.6 ribonuclease PH (NCBI) compare
b4646 yrdE +0.7 1.7 no description compare
b1563 relE +0.7 1.1 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b4141 yjeH +0.7 4.9 predicted transporter (NCBI) compare
b2433 b2433 +0.7 1.4 orf, hypothetical protein (VIMSS) compare
b1324 tpx +0.7 1.5 thiol peroxidase (NCBI) compare
b2327 yfcA +0.7 2.3 conserved inner membrane protein (NCBI) compare
b0183 rnhB +0.7 2.0 ribonuclease HII (NCBI) compare
b1000 cbpA +0.7 4.4 curved DNA-binding protein, DnaJ homologue that functions as a co-chaperone of DnaK (NCBI) compare
b1606 folM +0.7 3.2 short chain dehydrogenase (NCBI) compare
b3351 kefG +0.7 3.7 glutathione-regulated potassium-efflux system ancillary protein (NCBI) compare
b2513 yfgM +0.7 2.2 hypothetical protein (NCBI) compare
b1368 ynaA +0.7 1.9 Rac prophage; predicted tail protein (RefSeq) compare
b1426 ydcH +0.7 1.0 orf, hypothetical protein (VIMSS) compare
b3153 yhbO +0.7 1.7 orf, hypothetical protein (VIMSS) compare
b3527 yhjJ +0.7 5.5 predicted zinc-dependent peptidase (NCBI) compare
b1954 dsrA +0.7 1.8 regulatory antisense RNA (NCBI) compare
b0436 tig +0.7 3.3 trigger factor (NCBI) compare
b4090 rpiB +0.7 1.2 ribose-5-phosphate isomerase B (NCBI) compare
b2247 yfaW +0.7 3.1 predicted enolase (RefSeq) compare
b1952 dsrB +0.7 1.4 hypothetical protein (NCBI) compare
b3769 ilvM +0.7 2.5 acetolactate synthase II, small subunit (NCBI) compare
b1566 flxA +0.7 1.4 Qin prophage; predicted protein (NCBI) compare
b1961 dcm +0.7 1.2 DNA cytosine methylase (NCBI) compare
b1233 ychJ +0.7 0.9 hypothetical protein (NCBI) compare
b4649 ysaD +0.7 1.9 no description compare
b2910 zapA +0.7 2.2 protein that localizes to the cytokinetic ring (NCBI) compare
b2263 yfbB +0.7 1.8 predicted peptidase (NCBI) compare
b1726 yniB +0.6 0.7 predicted inner membrane protein (NCBI) compare
b3628 rfaB +0.6 3.8 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b0660 ybeZ +0.6 1.5 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b2963 mltC +0.6 4.3 membrane-bound lytic murein transglycosylase C (VIMSS) compare
b1220 ychO +0.6 1.9 predicted invasin (RefSeq) compare
b0686 ybfF +0.6 2.8 hypothetical protein (NCBI) compare
b2614 grpE +0.6 1.4 heat shock protein (NCBI) compare
b0209 yafD +0.6 2.2 hypothetical protein (NCBI) compare
b2576 srmB +0.6 2.0 ATP-dependent RNA helicase (NCBI) compare
b1066 rimJ +0.6 1.9 ribosomal-protein-S5-alanine N-acetyltransferase (NCBI) compare
b3250 mreC +0.6 0.7 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare


Specific Phenotypes

For 6 genes in this experiment

For carbon source D-Glucose in Escherichia coli BW25113

For carbon source D-Glucose across organisms