Experiment set40IT063 for Escherichia coli BW25113

Compare to:

carbon source D-Mannose 5mM

200 most detrimental genes:

  gene name fitness t score description  
b2904 gcvH +5.0 15.0 glycine cleavage system protein H (NCBI) compare
b2903 gcvP +4.8 43.5 glycine dehydrogenase (NCBI) compare
b3163 nlpI +4.6 22.0 hypothetical protein (NCBI) compare
b2905 gcvT +4.3 29.3 glycine cleavage system aminomethyltransferase T (NCBI) compare
b3405 ompR +4.3 19.4 osmolarity response regulator (NCBI) compare
b1830 prc +4.1 21.6 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2951 yggS +3.5 24.9 predicted enzyme (NCBI) compare
b0222 lpcA +3.0 10.5 phosphoheptose isomerase (NCBI) compare
b3620 rfaF +2.9 15.6 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3621 rfaC +2.8 16.6 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0053 surA +2.6 16.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b4042 dgkA +2.6 5.6 diacylglycerol kinase (NCBI) compare
b3753 rbsR +2.6 18.4 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2572 rseA +2.6 5.1 anti-sigma factor (NCBI) compare
b3052 rfaE +2.6 12.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0438 clpX +2.5 15.6 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3619 rfaD +2.5 9.8 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3630 rfaP +2.5 19.8 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3194 yrbE +2.5 6.8 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1863 ruvC +2.4 7.5 Holliday junction resolvase (NCBI) compare
b0437 clpP +2.4 10.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3199 yrbK +2.4 4.9 hypothetical protein (NCBI) compare
b3261 fis +2.4 8.9 DNA-binding protein Fis (NCBI) compare
b0178 hlpA +2.3 6.2 periplasmic chaperone (NCBI) compare
b3842 rfaH +2.3 14.0 transcriptional activator RfaH (NCBI) compare
b3787 rffD +2.3 18.5 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3404 envZ +2.3 12.5 osmolarity sensor protein (NCBI) compare
b3784 rfe +2.2 17.4 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3631 rfaG +2.2 18.8 glucosyltransferase I (NCBI) compare
b2808 gcvA +2.2 6.3 DNA-binding transcriptional dual regulator (NCBI) compare
b2742 nlpD +2.2 11.8 predicted outer membrane lipoprotein (NCBI) compare
b1332 ynaJ +2.1 1.5 predicted inner membrane protein (NCBI) compare
b3786 rffE +2.1 16.0 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b4402 yjjY +2.0 1.1 hypothetical protein (NCBI) compare
b0632 dacA +2.0 11.7 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b4701 sokX +2.0 1.7 sok-related sRNA, function unknown (RefSeq) compare
b2829 ptsP +2.0 12.5 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b4450 arcZ +2.0 7.2 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b3794 rffM +1.9 13.2 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b0957 ompA +1.9 5.2 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b3785 wzzE +1.9 14.0 putative transport protein (VIMSS) compare
b2188 yejM +1.9 11.1 predicted hydrolase, inner membrane (NCBI) compare
b3150 yraP +1.8 10.7 hypothetical protein (NCBI) compare
b2144 sanA +1.8 12.8 hypothetical protein (NCBI) compare
b3791 rffA +1.8 10.3 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b4203 rplI +1.8 9.0 50S ribosomal protein L9 (NCBI) compare
b4481 rffT +1.7 9.3 4-alpha-L-fucosyltransferase (NCBI) compare
b4169 amiB +1.7 11.8 N-acetylmuramoyl-l-alanine amidase II (NCBI) compare
b0092 ddlB +1.7 6.7 D-alanylalanine synthetase (NCBI) compare
b3181 greA +1.7 7.3 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3229 sspA +1.6 4.9 stringent starvation protein A (NCBI) compare
b0912 ihfB +1.6 7.0 integration host factor subunit beta (NCBI) compare
b3164 pnp +1.6 4.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3790 rffC +1.6 7.6 TDP-fucosamine acetyltransferase (RefSeq) compare
b3234 degQ +1.5 9.6 serine endoprotease, periplasmic (NCBI) compare
b3655 yicH +1.5 12.8 hypothetical protein (NCBI) compare
b0051 ksgA +1.5 6.4 dimethyladenosine transferase (NCBI) compare
b0688 pgm +1.5 4.9 phosphoglucomutase (NCBI) compare
b3472 b3472 +1.5 9.1 orf, hypothetical protein (VIMSS) compare
b2494 yfgC +1.5 9.1 predicted peptidase (NCBI) compare
b2017 yefM +1.5 1.0 orf, hypothetical protein (VIMSS) compare
b1524 yneH +1.5 1.5 predicted glutaminase (NCBI) compare
b2512 yfgL +1.4 6.1 protein assembly complex, lipoprotein component (NCBI) compare
b1095 fabF +1.4 7.8 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b0502 ylbG +1.4 1.9 no description compare
b3388 damX +1.4 11.7 hypothetical protein (NCBI) compare
b3206 npr +1.4 6.2 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b0929 ompF +1.3 5.2 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b0889 lrp +1.3 2.7 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1278 pgpB +1.3 2.2 phosphatidylglycerophosphatase B (NCBI) compare
b2783 chpR +1.3 1.6 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b1628 rsxB +1.3 2.3 electron transport complex protein RnfB (NCBI) compare
b3529 yhjK +1.3 10.1 predicted diguanylate cyclase (RefSeq) compare
b4505 insX +1.3 1.8 predicted IS protein (RefSeq) compare
b4552 yrhC +1.3 1.4 no description compare
b0059 hepA +1.3 10.7 ATP-dependent helicase HepA (NCBI) compare
b4699 fnrS +1.3 1.3 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b4172 hfq +1.3 2.0 RNA-binding protein Hfq (NCBI) compare
b2688 gshA +1.3 6.5 glutamate--cysteine ligase (NCBI) compare
b3443 yrhA +1.3 1.3 orf, hypothetical protein (VIMSS) compare
b1108 ycfP +1.2 2.1 orf, hypothetical protein (VIMSS) compare
b1150 ymfR +1.2 1.3 e14 prophage; predicted protein (NCBI) compare
b1677 lpp +1.2 4.6 murein lipoprotein (NCBI) compare
b2110 yehC +1.2 2.0 predicted periplasmic pilin chaperone (NCBI) compare
b1293 sapB +1.2 1.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0630 lipB +1.2 1.9 protein of lipoate biosynthesis (VIMSS) compare
b4141 yjeH +1.2 8.1 predicted transporter (NCBI) compare
b2830 nudH +1.2 1.8 dinucleoside polyphosphate hydrolase (NCBI) compare
b2743 pcm +1.1 4.4 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b3027 ygiZ +1.1 1.0 conserved inner membrane protein (NCBI) compare
b3341 rpsG +1.1 1.0 30S ribosomal protein S7 (NCBI) compare
b1358 ydaT +1.1 1.8 Rac prophage; predicted protein (NCBI) compare
b4375 prfC +1.1 3.8 peptide chain release factor 3 (NCBI) compare
b2362 yfdS +1.1 4.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1861 ruvA +1.1 1.7 Holliday junction DNA helicase motor protein (NCBI) compare
b2285 nuoE +1.1 1.6 NADH dehydrogenase subunit E (NCBI) compare
b3838 tatB +1.1 4.9 sec-independent translocase (NCBI) compare
b3210 arcB +1.1 4.9 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b1556 b1556 +1.1 2.0 orf, hypothetical protein (VIMSS) compare
b1824 yobF +1.1 2.6 hypothetical protein (NCBI) compare
b1984 asnW +1.1 1.4 tRNA-Asn (NCBI) compare
b2183 rsuA +1.0 3.0 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b2946 yggJ +1.0 3.5 orf, hypothetical protein (VIMSS) compare
b2352 yfdI +1.0 1.3 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b4596 yciZ +1.0 0.9 hypothetical protein (RefSeq) compare
b2279 nuoK +1.0 0.8 NADH dehydrogenase subunit K (NCBI) compare
b4171 miaA +1.0 2.6 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b3643 rph +1.0 3.8 ribonuclease PH (NCBI) compare
b3260 dusB +1.0 3.9 tRNA-dihydrouridine synthase B (NCBI) compare
b3198 kdsC +1.0 2.5 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2620 smpB +1.0 0.9 SsrA-binding protein (NCBI) compare
b1183 umuD +1.0 1.0 DNA polymerase V, subunit D (NCBI) compare
b1271 yciK +1.0 2.1 short chain dehydrogenase (NCBI) compare
b0791 ybhQ +0.9 4.0 predicted inner membrane protein (NCBI) compare
b4202 rpsR +0.9 1.1 30S ribosomal protein S18 (NCBI) compare
b3752 rbsK +0.9 4.4 ribokinase (NCBI) compare
b1630 rsxD +0.9 2.8 electron transport complex protein RnfD (NCBI) compare
b2297 pta +0.9 0.5 phosphate acetyltransferase (NCBI) compare
b4436 ryeC +0.9 1.7 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b0843 ybjH +0.9 1.4 hypothetical protein (NCBI) compare
b4642 yoeD +0.9 2.0 no description compare
b1217 chaB +0.9 1.4 cation transport regulator (NCBI) compare
b2432 yfeY +0.9 2.1 hypothetical protein (NCBI) compare
b1814 sdaA +0.9 2.1 L-serine deaminase I (NCBI) compare
b1038 csgF +0.9 2.2 predicted transport protein (NCBI) compare
b4653 yicT +0.9 0.9 no description compare
b4546 ypeB +0.9 1.0 hypothetical protein (NCBI) compare
b3911 cpxA +0.9 5.7 two-component sensor protein (NCBI) compare
b2516 yfgA +0.9 3.6 hypothetical protein (NCBI) compare
b1572 ydfB +0.9 1.4 orf, hypothetical protein (VIMSS) compare
b2947 gshB +0.9 3.0 glutathione synthetase (NCBI) compare
b2846 yqeH +0.9 0.4 orf, hypothetical protein (VIMSS) compare
b1884 cheR +0.9 2.3 chemotaxis regulator, protein-glutamate methyltransferase (NCBI) compare
b4163 glyV +0.9 0.7 tRNA-Gly (NCBI) compare
b1575 dicB +0.8 0.8 Qin prophage; cell division inhibition protein (NCBI) compare
b1636 pdxY +0.8 2.7 pyridoxine kinase (NCBI) compare
b2064 asmA +0.8 4.3 predicted assembly protein (NCBI) compare
b0121 speE +0.8 3.7 spermidine synthase (NCBI) compare
b1493 gadB +0.8 0.7 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1953 b1953 +0.8 2.3 orf, hypothetical protein (VIMSS) compare
b2141 yohJ +0.8 1.4 hypothetical protein (NCBI) compare
b4594 ymgJ +0.8 1.7 hypothetical protein (RefSeq) compare
b1604 ydgH +0.8 3.3 hypothetical protein (NCBI) compare
b4100 phnH +0.8 2.9 carbon-phosphorus lyase complex subunit (NCBI) compare
b3977 tyrU +0.8 0.8 tRNA-Tyr (NCBI) compare
b1874 cutC +0.8 1.8 copper homeostasis protein (NCBI) compare
b2278 nuoL +0.8 3.8 NADH dehydrogenase subunit L (NCBI) compare
b3351 kefG +0.8 3.6 glutathione-regulated potassium-efflux system ancillary protein (NCBI) compare
b3192 yrbC +0.8 4.5 predicted ABC-type organic solvent transporter (NCBI) compare
b4181 yjfI +0.8 2.0 hypothetical protein (NCBI) compare
b0249 ykfF +0.8 1.6 CP4-6 prophage; predicted protein (NCBI) compare
b1503 ydeR +0.8 2.4 predicted fimbrial-like adhesin protein (NCBI) compare
b2066 udk +0.8 2.9 uridine/cytidine kinase (VIMSS) compare
b0633 rlpA +0.8 4.2 minor lipoprotein (NCBI) compare
b3197 kdsD +0.8 4.0 D-arabinose 5-phosphate isomerase (NCBI) compare
b4592 yccB +0.8 1.6 hypothetical protein (RefSeq) compare
b2741 rpoS +0.8 2.8 RNA polymerase sigma factor (NCBI) compare
b0430 cyoC +0.8 2.6 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b2287 nuoB +0.8 2.1 NADH dehydrogenase subunit B (NCBI) compare
b3193 yrbD +0.8 4.4 predicted ABC-type organic solvent transporter (NCBI) compare
b2258 b2258 +0.8 0.8 putative transport/receptor protein (VIMSS) compare
b0882 clpA +0.8 3.5 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b2346 vacJ +0.8 4.4 predicted lipoprotein (NCBI) compare
b2286 nuoC +0.8 2.7 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b4472 yhdP +0.8 6.1 conserved membrane protein, predicted transporter (NCBI) compare
b1606 folM +0.8 3.7 short chain dehydrogenase (NCBI) compare
b4373 rimI +0.8 1.5 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1634 tppB +0.7 2.9 putative tripeptide transporter permease (NCBI) compare
b0247 ykfG +0.7 0.9 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b2277 nuoM +0.7 2.5 NADH dehydrogenase subunit M (NCBI) compare
b4155 yjeA +0.7 0.8 putative lysyl-tRNA synthetase (VIMSS) compare
b3484 yhhI +0.7 1.8 predicted transposase (NCBI) compare
b1222 narX +0.7 2.9 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b3836 b3836 +0.7 2.6 orf, hypothetical protein (VIMSS) compare
b1190 dadX +0.7 2.6 alanine racemase (NCBI) compare
b3632 rfaQ +0.7 6.1 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0197 metQ +0.7 4.3 DL-methionine transporter subunit (NCBI) compare
b1482 osmC +0.7 1.3 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b4391 yjjK +0.7 4.2 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2377 yfdY +0.7 1.7 predicted inner membrane protein (NCBI) compare
b3652 recG +0.7 3.7 ATP-dependent DNA helicase (NCBI) compare
b0542 renD +0.7 1.5 orf, hypothetical protein (VIMSS) compare
b0295 ykgL +0.7 3.2 hypothetical protein (NCBI) compare
b1422 ydcI +0.7 4.1 putative transcriptional regulator LYSR-type (VIMSS) compare
b0432 cyoA +0.7 4.5 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b0852 rimK +0.7 2.7 ribosomal protein S6 modification protein (NCBI) compare
b3191 yrbB +0.7 4.2 orf, hypothetical protein (VIMSS) compare
b4558 yifL +0.7 1.6 predicted lipoprotein (NCBI) compare
b2635 ypjK +0.7 1.5 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1793 yoaF +0.7 1.1 conserved outer membrane protein (NCBI) compare
b1712 ihfA +0.7 1.3 integration host factor subunit alpha (NCBI) compare
b1013 ycdC +0.7 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3021 ygiT +0.7 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b0429 cyoD +0.7 1.8 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b0738 tolR +0.7 1.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2817 b2817 +0.7 2.4 putative amidase (VIMSS) compare
b3195 yrbF +0.7 4.1 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2058 wcaB +0.7 0.8 predicted acyl transferase (NCBI) compare
b0381 ddlA +0.7 3.5 D-alanylalanine synthetase (NCBI) compare
b0741 pal +0.7 2.6 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare


Specific Phenotypes

For 11 genes in this experiment

For carbon source D-Mannose in Escherichia coli BW25113

For carbon source D-Mannose across organisms