Experiment set40IT061 for Escherichia coli BW25113

Compare to:

carbon source D-Glucose 5mM

200 most detrimental genes:

  gene name fitness t score description  
b3194 yrbE +3.8 7.5 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2904 gcvH +3.8 10.4 glycine cleavage system protein H (NCBI) compare
b4042 dgkA +3.7 7.4 diacylglycerol kinase (NCBI) compare
b3405 ompR +3.7 11.3 osmolarity response regulator (NCBI) compare
b2903 gcvP +3.6 25.3 glycine dehydrogenase (NCBI) compare
b3021 ygiT +3.5 4.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2905 gcvT +3.4 20.9 glycine cleavage system aminomethyltransferase T (NCBI) compare
b1830 prc +3.1 17.7 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2144 sanA +3.0 20.0 hypothetical protein (NCBI) compare
b2846 yqeH +2.9 1.5 orf, hypothetical protein (VIMSS) compare
b3082 ygjM +2.8 3.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2808 gcvA +2.8 10.0 DNA-binding transcriptional dual regulator (NCBI) compare
b4647 mokA +2.6 1.3 no description compare
b0232 yafN +2.5 2.2 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3404 envZ +2.5 13.3 osmolarity sensor protein (NCBI) compare
b3163 nlpI +2.5 8.1 hypothetical protein (NCBI) compare
b2188 yejM +2.4 11.2 predicted hydrolase, inner membrane (NCBI) compare
b3164 pnp +2.3 5.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0438 clpX +2.3 8.3 ATP-dependent protease ATP-binding subunit (NCBI) compare
b2064 asmA +2.3 10.7 predicted assembly protein (NCBI) compare
b0632 dacA +2.3 9.3 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b0437 clpP +2.3 7.6 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b2894 xerD +2.2 2.3 site-specific tyrosine recombinase XerD (NCBI) compare
b2017 yefM +2.2 1.5 orf, hypothetical protein (VIMSS) compare
b0738 tolR +2.1 5.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3620 rfaF +2.1 7.6 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3630 rfaP +2.0 13.2 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2951 yggS +2.0 5.5 predicted enzyme (NCBI) compare
b3261 fis +2.0 3.3 DNA-binding protein Fis (NCBI) compare
b2742 nlpD +2.0 7.8 predicted outer membrane lipoprotein (NCBI) compare
b0912 ihfB +2.0 2.9 integration host factor subunit beta (NCBI) compare
b4169 amiB +2.0 11.8 N-acetylmuramoyl-l-alanine amidase II (NCBI) compare
b1824 yobF +1.9 4.2 hypothetical protein (NCBI) compare
b1863 ruvC +1.9 4.6 Holliday junction resolvase (NCBI) compare
b3341 rpsG +1.9 1.7 30S ribosomal protein S7 (NCBI) compare
b0802 ybiJ +1.9 2.0 hypothetical protein (NCBI) compare
b4585 sroB +1.8 1.9 Novel sRNA, function unknown (RefSeq) compare
b3842 rfaH +1.8 5.5 transcriptional activator RfaH (NCBI) compare
b3199 yrbK +1.8 3.2 hypothetical protein (NCBI) compare
b4570 lomR +1.7 1.9 no description compare
b4520 ymgF +1.7 1.8 hypothetical protein (NCBI) compare
b4546 ypeB +1.6 1.5 hypothetical protein (NCBI) compare
b1874 cutC +1.6 3.1 copper homeostasis protein (NCBI) compare
b0729 sucD +1.6 2.7 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2939 yqgB +1.6 1.4 predicted protein (RefSeq) compare
b3387 dam +1.6 9.9 DNA adenine methylase (NCBI) compare
b2688 gshA +1.6 7.6 glutamate--cysteine ligase (NCBI) compare
b1108 ycfP +1.6 1.7 orf, hypothetical protein (VIMSS) compare
b1273 yciN +1.5 2.2 hypothetical protein (NCBI) compare
b4202 rpsR +1.5 1.4 30S ribosomal protein S18 (NCBI) compare
b4203 rplI +1.5 4.7 50S ribosomal protein L9 (NCBI) compare
b2829 ptsP +1.5 9.1 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1418 cybB +1.5 2.4 cytochrome b(561) (VIMSS) compare
b0706 ybfD +1.5 2.5 hypothetical protein (NCBI) compare
b3621 rfaC +1.5 5.2 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3631 rfaG +1.4 10.7 glucosyltransferase I (NCBI) compare
b2684 mprA +1.4 2.6 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2291 yfbR +1.4 4.1 hypothetical protein (NCBI) compare
b2659 b2659 +1.4 2.3 orf, hypothetical protein (VIMSS) compare
b0059 hepA +1.4 9.7 ATP-dependent helicase HepA (NCBI) compare
b2830 nudH +1.4 1.5 dinucleoside polyphosphate hydrolase (NCBI) compare
b2516 yfgA +1.4 5.3 hypothetical protein (NCBI) compare
b0991 ymcE +1.4 3.9 cold shock gene (NCBI) compare
b2237 inaA +1.3 1.6 hypothetical protein (NCBI) compare
b3652 recG +1.3 6.2 ATP-dependent DNA helicase (NCBI) compare
b1778 yeaA +1.3 1.5 methionine sulfoxide reductase B (NCBI) compare
b0927 ycbL +1.3 2.2 predicted metal-binding enzyme (NCBI) compare
b4215 ytfI +1.3 2.7 hypothetical protein (NCBI) compare
b2104 thiM +1.3 1.2 hydroxyethylthiazole kinase (NCBI) compare
b1143 ymfI +1.3 1.6 orf, hypothetical protein (VIMSS) compare
b0339 cynT +1.3 2.5 carbonic anhydrase (NCBI) compare
b3234 degQ +1.3 5.6 serine endoprotease, periplasmic (NCBI) compare
b3052 rfaE +1.3 3.6 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2642 yfjW +1.3 0.8 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b4593 ymgI +1.2 0.8 hypothetical protein (RefSeq) compare
b3935 priA +1.2 1.0 primosome assembly protein PriA (NCBI) compare
b1754 b1754 +1.2 1.2 orf, hypothetical protein (VIMSS) compare
b1589 ynfG +1.2 1.5 oxidoreductase, Fe-S subunit (NCBI) compare
b3206 npr +1.2 3.4 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2352 yfdI +1.2 1.5 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b1594 dgsA +1.2 2.6 DNA-binding transcriptional repressor (NCBI) compare
b1446 ydcY +1.2 1.4 hypothetical protein (NCBI) compare
b4253 yjgL +1.2 4.2 orf, hypothetical protein (VIMSS) compare
b1642 slyA +1.2 1.8 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1278 pgpB +1.2 1.8 phosphatidylglycerophosphatase B (NCBI) compare
b2646 ypjF +1.2 2.3 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b1153 ymfQ +1.2 2.6 e14 prophage; conserved protein (NCBI) compare
b1222 narX +1.2 3.5 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b1689 b1689 +1.2 2.9 orf, hypothetical protein (VIMSS) compare
b1105 ycfM +1.1 1.4 predicted outer membrane lipoprotein (NCBI) compare
b2698 recX +1.1 2.0 RecA regulator RecX (NCBI) compare
b0092 ddlB +1.1 2.9 D-alanylalanine synthetase (NCBI) compare
b1236 galU +1.1 3.5 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1686 ydiI +1.1 1.5 hypothetical protein (NCBI) compare
b1104 ycfL +1.1 1.7 hypothetical protein (NCBI) compare
b2572 rseA +1.1 1.8 anti-sigma factor (NCBI) compare
b1408 b1408 +1.1 2.7 probable enzyme (VIMSS) compare
b4097 phnK +1.1 2.6 carbon-phosphorus lyase complex subunit (NCBI) compare
b0101 yacG +1.1 2.2 zinc-binding protein (NCBI) compare
b1602 pntB +1.1 2.5 pyridine nucleotide transhydrogenase (NCBI) compare
b2897 ygfY +1.1 1.9 hypothetical protein (NCBI) compare
b4596 yciZ +1.1 0.7 hypothetical protein (RefSeq) compare
b1960 vsr +1.1 1.7 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b3786 rffE +1.1 6.4 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1163 ycgF +1.1 3.6 predicted FAD-binding phosphodiesterase (NCBI) compare
b2123 yehR +1.1 2.0 orf, hypothetical protein (VIMSS) compare
b0088 murD +1.1 1.8 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b1613 manA +1.1 1.3 mannose-6-phosphate isomerase (VIMSS) compare
b3778 rep +1.1 4.1 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2080 b2080 +1.0 1.3 orf, hypothetical protein (VIMSS) compare
b1346 ydaQ +1.0 1.4 orf, hypothetical protein (VIMSS) compare
b3129 sohA +1.0 1.8 predicted regulator (NCBI) compare
b1751 ydjY +1.0 2.0 orf, hypothetical protein (VIMSS) compare
b4431 rprA +1.0 1.3 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b3787 rffD +1.0 5.2 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b2531 iscR +1.0 0.7 DNA-binding transcriptional repressor (NCBI) compare
b2743 pcm +1.0 2.1 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b2066 udk +1.0 2.1 uridine/cytidine kinase (VIMSS) compare
b0563 tfaX +1.0 2.9 DLP12 prophage; predicted protein (RefSeq) compare
b2356 yfdM +1.0 1.3 orf, hypothetical protein (VIMSS) compare
b4655 ythA +1.0 0.7 expressed protein (RefSeq) compare
b1013 ycdC +1.0 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3071 yqjI +1.0 2.1 predicted transcriptional regulator (NCBI) compare
b0683 fur +1.0 0.9 ferric uptake regulator (NCBI) compare
b1959 yedA +1.0 2.6 predicted inner membrane protein (NCBI) compare
b3229 sspA +1.0 2.2 stringent starvation protein A (NCBI) compare
b3388 damX +1.0 6.7 hypothetical protein (NCBI) compare
b2299 yfcD +1.0 1.6 predicted NUDIX hydrolase (NCBI) compare
b2637 yfjT +1.0 1.7 CP4-57 prophage; predicted protein (NCBI) compare
b0219 yafV +1.0 2.9 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b0728 sucC +0.9 2.9 succinyl-CoA synthetase subunit beta (NCBI) compare
b0520 ylbF +0.9 2.8 hypothetical protein (NCBI) compare
b2455 eutE +0.9 1.7 predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI) compare
b1339 abgR +0.9 2.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1949 fliQ +0.9 1.5 flagellar biosynthesis protein Q (NCBI) compare
b1756 ynjD +0.9 2.2 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2570 rseC +0.9 2.4 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b2325 yfcL +0.9 1.0 hypothetical protein (NCBI) compare
b2136 yohD +0.9 0.9 orf, hypothetical protein (VIMSS) compare
b1271 yciK +0.9 1.3 short chain dehydrogenase (NCBI) compare
b1383 ydbL +0.9 1.2 orf, hypothetical protein (VIMSS) compare
b1107 nagZ +0.9 2.2 beta-hexosaminidase (NCBI) compare
b0521 ybcF +0.9 1.7 predicted carbamate kinase (NCBI) compare
b2947 gshB +0.9 2.4 glutathione synthetase (NCBI) compare
b1806 yeaY +0.9 1.6 predicted lipoprotein (NCBI) compare
b1677 lpp +0.9 1.7 murein lipoprotein (NCBI) compare
b3150 yraP +0.9 2.8 hypothetical protein (NCBI) compare
b0596 entA +0.9 2.7 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI) compare
b2285 nuoE +0.9 1.6 NADH dehydrogenase subunit E (NCBI) compare
b2259 pmrD +0.9 0.7 polymyxin resistance protein B (VIMSS) compare
b1305 pspB +0.9 1.4 phage shock protein B (NCBI) compare
b0710 ybgI +0.9 2.5 conserved metal-binding protein (NCBI) compare
b4268 idnK +0.9 2.6 D-gluconate kinase, thermosensitive (NCBI) compare
b2625 yfjI +0.9 1.6 CP4-57 prophage; predicted protein (NCBI) compare
b0310 ykgH +0.9 1.4 predicted inner membrane protein (NCBI) compare
b1609 rstB +0.9 2.1 sensory histidine kinase in two-component regulatory system with RstA (NCBI) compare
b3785 wzzE +0.9 5.3 putative transport protein (VIMSS) compare
b2490 b2490 +0.9 0.8 putative protein processing element (VIMSS) compare
b2193 narP +0.9 1.9 DNA-binding response regulator in two-component regulatory system with NarQ or NarX (NCBI) compare
b1634 tppB +0.9 2.5 putative tripeptide transporter permease (NCBI) compare
b1454 yncG +0.9 1.6 predicted enzyme (NCBI) compare
b3614 yibQ +0.9 4.7 orf, hypothetical protein (VIMSS) compare
b4505 insX +0.9 0.7 predicted IS protein (RefSeq) compare
b2012 yeeD +0.9 0.8 hypothetical protein (NCBI) compare
b4649 ysaD +0.9 1.8 no description compare
b0955 ycbZ +0.9 3.4 predicted peptidase (NCBI) compare
b1336 ydaH +0.9 1.7 putative pump protein (transport) (VIMSS) compare
b1855 lpxM +0.9 3.2 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4030 yjbA +0.9 2.3 phosphate-starvation-inducible protein PsiE (NCBI) compare
b3545 proK +0.9 1.5 tRNA-Pro (NCBI) compare
b1811 yoaH +0.8 1.5 hypothetical protein (NCBI) compare
b0220 ivy +0.8 2.2 inhibitor of vertebrate C-lysozyme (NCBI) compare
b0784 moaD +0.8 1.3 molybdopterin synthase, small subunit (NCBI) compare
b1761 gdhA +0.8 2.1 glutamate dehydrogenase (NCBI) compare
b0926 ycbK +0.8 3.6 hypothetical protein (NCBI) compare
b1989 asnV +0.8 1.3 tRNA-Asn (NCBI) compare
b0471 ybaB +0.8 1.6 hypothetical protein (NCBI) compare
b0980 appA +0.8 3.1 phosphoanhydride phosphorylase (NCBI) compare
b2768 ygcP +0.8 1.8 predicted anti-terminator regulatory protein (NCBI) compare
b3791 rffA +0.8 3.2 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2175 spr +0.8 1.6 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b1650 nemA +0.8 1.7 N-ethylmaleimide reductase, FMN-linked (NCBI) compare
b4535 yniD +0.8 0.8 predicted protein (RefSeq) compare
b4656 yjhY +0.8 1.3 no description compare
b2636 b2636 +0.8 2.2 orf, hypothetical protein (VIMSS) compare
b0712 ybgK +0.8 2.6 predicted enzyme subunit (NCBI) compare
b3977 tyrU +0.8 0.6 tRNA-Tyr (NCBI) compare
b2425 cysP +0.8 3.0 thiosulfate transporter subunit (NCBI) compare
b3035 tolC +0.8 3.5 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0641 rlpB +0.8 1.5 minor lipoprotein (NCBI) compare
b3203 yhbH +0.8 1.6 predicted ribosome-associated, sigma 54 modulation protein (NCBI) compare
b3643 rph +0.8 2.9 ribonuclease PH (NCBI) compare
b1248 b1248 +0.8 1.4 orf, hypothetical protein (VIMSS) compare
b1583 ynfB +0.8 1.3 hypothetical protein (NCBI) compare
b2047 wcaJ +0.8 2.1 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b1390 paaC +0.8 1.8 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b2426 ucpA +0.8 1.3 putative oxidoreductase (VIMSS) compare
b2457 cchA +0.8 1.2 detox protein (VIMSS) compare
b0014 dnaK +0.8 1.1 molecular chaperone DnaK (NCBI) compare
b2832 ygdQ +0.8 2.6 predicted inner membrane protein (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For carbon source D-Glucose in Escherichia coli BW25113

For carbon source D-Glucose across organisms