Experiment set40IT059 for Escherichia coli BW25113

Compare to:

carbon source D-Mannose 20mM

200 most detrimental genes:

  gene name fitness t score description  
b2904 gcvH +4.6 18.3 glycine cleavage system protein H (NCBI) compare
b2903 gcvP +4.1 35.9 glycine dehydrogenase (NCBI) compare
b2905 gcvT +3.6 21.1 glycine cleavage system aminomethyltransferase T (NCBI) compare
b2951 yggS +3.5 21.7 predicted enzyme (NCBI) compare
b1830 prc +3.4 19.0 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3163 nlpI +3.4 13.8 hypothetical protein (NCBI) compare
b2572 rseA +3.2 6.1 anti-sigma factor (NCBI) compare
b3261 fis +2.8 9.4 DNA-binding protein Fis (NCBI) compare
b3082 ygjM +2.8 4.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3911 cpxA +2.8 19.6 two-component sensor protein (NCBI) compare
b2829 ptsP +2.8 20.2 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3405 ompR +2.8 10.8 osmolarity response regulator (NCBI) compare
b0178 hlpA +2.7 13.9 periplasmic chaperone (NCBI) compare
b4042 dgkA +2.7 8.1 diacylglycerol kinase (NCBI) compare
b3784 rfe +2.7 22.2 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3206 npr +2.5 13.5 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b3472 b3472 +2.5 19.9 orf, hypothetical protein (VIMSS) compare
b0053 surA +2.4 16.0 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2808 gcvA +2.4 11.6 DNA-binding transcriptional dual regulator (NCBI) compare
b0222 lpcA +2.3 10.5 phosphoheptose isomerase (NCBI) compare
b3787 rffD +2.3 18.5 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3052 rfaE +2.3 9.6 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3786 rffE +2.3 19.0 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3620 rfaF +2.3 12.6 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3621 rfaC +2.2 14.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3529 yhjK +2.2 16.9 predicted diguanylate cyclase (RefSeq) compare
b3655 yicH +2.2 19.1 hypothetical protein (NCBI) compare
b3794 rffM +2.2 13.6 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1677 lpp +2.1 7.4 murein lipoprotein (NCBI) compare
b3753 rbsR +2.1 15.7 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2830 nudH +2.0 3.6 dinucleoside polyphosphate hydrolase (NCBI) compare
b3164 pnp +2.0 5.2 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4458 oxyS +1.9 3.1 global regulatory RNA (NCBI) compare
b0059 hepA +1.8 15.6 ATP-dependent helicase HepA (NCBI) compare
b2017 yefM +1.7 2.9 orf, hypothetical protein (VIMSS) compare
b3785 wzzE +1.7 11.7 putative transport protein (VIMSS) compare
b0632 dacA +1.7 10.1 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3181 greA +1.7 8.5 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b4203 rplI +1.7 7.5 50S ribosomal protein L9 (NCBI) compare
b3643 rph +1.6 5.0 ribonuclease PH (NCBI) compare
b0437 clpP +1.6 7.8 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b4481 rffT +1.6 10.2 4-alpha-L-fucosyltransferase (NCBI) compare
b3790 rffC +1.5 10.5 TDP-fucosamine acetyltransferase (RefSeq) compare
b3619 rfaD +1.5 7.0 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2144 sanA +1.5 11.7 hypothetical protein (NCBI) compare
b3630 rfaP +1.5 11.5 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3021 ygiT +1.5 3.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1360 ydaV +1.5 2.0 Rac prophage; predicted DNA replication protein (NCBI) compare
b2494 yfgC +1.4 8.3 predicted peptidase (NCBI) compare
b3631 rfaG +1.4 12.2 glucosyltransferase I (NCBI) compare
b3791 rffA +1.4 9.5 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b4402 yjjY +1.4 1.6 hypothetical protein (NCBI) compare
b4592 yccB +1.4 1.9 hypothetical protein (RefSeq) compare
b2742 nlpD +1.3 7.4 predicted outer membrane lipoprotein (NCBI) compare
b1446 ydcY +1.3 2.9 hypothetical protein (NCBI) compare
b4171 miaA +1.3 3.6 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b3863 polA +1.3 2.1 DNA polymerase I (NCBI) compare
b0092 ddlB +1.3 7.0 D-alanylalanine synthetase (NCBI) compare
b2688 gshA +1.3 6.2 glutamate--cysteine ligase (NCBI) compare
b3842 rfaH +1.3 7.1 transcriptional activator RfaH (NCBI) compare
b1143 ymfI +1.3 3.0 orf, hypothetical protein (VIMSS) compare
b3229 sspA +1.2 4.5 stringent starvation protein A (NCBI) compare
b4048 yjbM +1.2 2.1 hypothetical protein (NCBI) compare
b1362 rzpR +1.2 0.9 putative Rac prophage endopeptidase (VIMSS) compare
b1095 fabF +1.2 6.2 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b4172 hfq +1.2 1.4 RNA-binding protein Hfq (NCBI) compare
b4702 mgtL +1.2 2.3 regulatory leader peptide for mgtA (NCBI) compare
b2259 pmrD +1.2 1.5 polymyxin resistance protein B (VIMSS) compare
b3426 glpD +1.1 3.3 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b1709 btuD +1.1 1.1 vitamin B12-transporter ATPase (NCBI) compare
b4410 ecnA +1.1 1.6 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b3260 dusB +1.1 3.9 tRNA-dihydrouridine synthase B (NCBI) compare
b2939 yqgB +1.1 2.2 predicted protein (RefSeq) compare
b2512 yfgL +1.1 4.8 protein assembly complex, lipoprotein component (NCBI) compare
b1524 yneH +1.0 1.1 predicted glutaminase (NCBI) compare
b1368 ynaA +1.0 2.1 Rac prophage; predicted tail protein (RefSeq) compare
b0438 clpX +1.0 4.9 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1542 ydfI +1.0 3.0 predicted mannonate dehydrogenase (NCBI) compare
b4416 rybA +1.0 1.8 unknown RNA (NCBI) compare
b2080 b2080 +1.0 2.1 orf, hypothetical protein (VIMSS) compare
b2141 yohJ +1.0 2.1 hypothetical protein (NCBI) compare
b1222 narX +1.0 5.5 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b4450 arcZ +1.0 2.9 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b0957 ompA +1.0 2.9 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b4169 amiB +1.0 6.3 N-acetylmuramoyl-l-alanine amidase II (NCBI) compare
b1661 cfa +1.0 4.8 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b1418 cybB +1.0 1.7 cytochrome b(561) (VIMSS) compare
b4144 yjeI +0.9 4.1 orf, hypothetical protein (VIMSS) compare
b3199 yrbK +0.9 1.6 hypothetical protein (NCBI) compare
b1618 uidR +0.9 2.1 DNA-binding transcriptional repressor (NCBI) compare
b1842 holE +0.9 1.0 DNA polymerase III, theta subunit (NCBI) compare
b1429 tehA +0.9 1.6 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b0082 mraW +0.9 2.6 S-adenosyl-methyltransferase (NCBI) compare
b1292 sapC +0.9 1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1293 sapB +0.9 1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0864 artP +0.9 1.8 arginine transporter subunit (NCBI) compare
b4701 sokX +0.9 0.7 sok-related sRNA, function unknown (RefSeq) compare
b0866 ybjQ +0.9 1.1 hypothetical protein (NCBI) compare
b1607 ydgC +0.9 1.6 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b4609 ryfD +0.9 2.5 Novel sRNA, function unknown (RefSeq) compare
b4375 prfC +0.9 3.7 peptide chain release factor 3 (NCBI) compare
b1949 fliQ +0.9 2.4 flagellar biosynthesis protein Q (NCBI) compare
b3627 rfaI +0.9 5.4 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2817 b2817 +0.9 3.5 putative amidase (VIMSS) compare
b3404 envZ +0.9 5.3 osmolarity sensor protein (NCBI) compare
b3198 kdsC +0.9 1.7 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b3210 arcB +0.8 4.6 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b4505 insX +0.8 0.8 predicted IS protein (RefSeq) compare
b4068 yjcH +0.8 2.7 required for glycolate transport, with actP (from data) compare
b1778 yeaA +0.8 1.9 methionine sulfoxide reductase B (NCBI) compare
b2317 dedA +0.8 2.0 conserved inner membrane protein (NCBI) compare
b1920 fliY +0.8 1.6 cystine transporter subunit (NCBI) compare
b3396 mrcA +0.8 7.4 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b1628 rsxB +0.8 2.9 electron transport complex protein RnfB (NCBI) compare
b1108 ycfP +0.8 2.4 orf, hypothetical protein (VIMSS) compare
b4546 ypeB +0.8 1.0 hypothetical protein (NCBI) compare
b0232 yafN +0.8 0.7 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0617 citD +0.8 1.5 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b0051 ksgA +0.8 2.8 dimethyladenosine transferase (NCBI) compare
b1150 ymfR +0.8 0.8 e14 prophage; predicted protein (NCBI) compare
b2007 yeeX +0.8 2.7 conserved protein (RefSeq) compare
b3628 rfaB +0.8 4.9 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b2620 smpB +0.8 0.8 SsrA-binding protein (NCBI) compare
b4294 insA-7 +0.8 1.6 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b2513 yfgM +0.8 2.6 hypothetical protein (NCBI) compare
b2637 yfjT +0.8 1.6 CP4-57 prophage; predicted protein (NCBI) compare
b2946 yggJ +0.8 2.2 orf, hypothetical protein (VIMSS) compare
b3150 yraP +0.8 5.0 hypothetical protein (NCBI) compare
b3632 rfaQ +0.8 6.6 lipopolysaccharide core biosynthesis protein (NCBI) compare
b3194 yrbE +0.8 4.1 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0596 entA +0.8 4.5 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI) compare
b4174 hflK +0.8 4.8 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1190 dadX +0.8 3.3 alanine racemase (NCBI) compare
b3121 yhaC +0.8 0.4 hypothetical protein (NCBI) compare
b2285 nuoE +0.8 1.4 NADH dehydrogenase subunit E (NCBI) compare
b0695 kdpD +0.8 3.1 fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein (NCBI) compare
b4598 yncL +0.8 1.8 hypothetical protein (RefSeq) compare
b2279 nuoK +0.7 0.8 NADH dehydrogenase subunit K (NCBI) compare
b2168 fruK +0.7 0.5 1-phosphofructokinase (NCBI) compare
b1630 rsxD +0.7 3.1 electron transport complex protein RnfD (NCBI) compare
b1085 b1085 +0.7 1.3 orf, hypothetical protein (VIMSS) compare
b4468 glcE +0.7 2.0 glycolate oxidase FAD binding subunit (NCBI) compare
b0563 tfaX +0.7 3.3 DLP12 prophage; predicted protein (RefSeq) compare
b1824 yobF +0.7 1.3 hypothetical protein (NCBI) compare
b4552 yrhC +0.7 0.8 no description compare
b4220 ytfM +0.7 5.6 predicted outer membrane protein and surface antigen (NCBI) compare
b2614 grpE +0.7 1.5 heat shock protein (NCBI) compare
b2363 yfdT +0.7 1.3 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1434 ydcN +0.7 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1620 malI +0.7 2.0 DNA-binding transcriptional repressor (NCBI) compare
b3034 nudF +0.7 3.1 ADP-ribose pyrophosphatase (NCBI) compare
b1007 b1007 +0.7 1.4 orf, hypothetical protein (VIMSS) compare
b1823 cspC +0.7 1.8 stress protein, member of the CspA-family (NCBI) compare
b4260 pepA +0.7 4.7 leucyl aminopeptidase (NCBI) compare
b4221 ytfN +0.7 6.0 hypothetical protein (NCBI) compare
b4155 yjeA +0.7 0.8 putative lysyl-tRNA synthetase (VIMSS) compare
b0796 ybiH +0.7 4.1 putative transcriptional regulator (VIMSS) compare
b1904 yecR +0.7 2.0 hypothetical protein (NCBI) compare
b2175 spr +0.7 2.4 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0979 appB +0.7 2.8 cytochrome bd-II oxidase, subunit II (NCBI) compare
b3147 yraM +0.7 5.2 hypothetical protein (NCBI) compare
b1467 narY +0.7 3.0 nitrate reductase 2 (NRZ), beta subunit (NCBI) compare
b3388 damX +0.7 5.4 hypothetical protein (NCBI) compare
b1382 ynbE +0.7 1.1 predicted lipoprotein (NCBI) compare
b1271 yciK +0.7 1.4 short chain dehydrogenase (NCBI) compare
b1648 b1648 +0.7 1.2 orf, hypothetical protein (VIMSS) compare
b1855 lpxM +0.7 3.5 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1412 azoR +0.7 1.9 acyl carrier protein phosphodiesterase (NCBI) compare
b0683 fur +0.7 0.9 ferric uptake regulator (NCBI) compare
b0729 sucD +0.7 1.8 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2947 gshB +0.7 2.6 glutathione synthetase (NCBI) compare
b1514 lsrC +0.7 1.1 AI2 transporter (NCBI) compare
b3035 tolC +0.6 3.4 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b4095 phnM +0.6 2.7 carbon-phosphorus lyase complex subunit (NCBI) compare
b1591 b1591 +0.6 1.5 putative oxidoreductase component (VIMSS) compare
b3107 yhaL +0.6 1.0 orf, hypothetical protein (VIMSS) compare
b2700 ygaD +0.6 3.2 competence damage-inducible protein A (NCBI) compare
b1548 nohA +0.6 0.8 Qin prophage; predicted packaging protein (RefSeq) compare
b0784 moaD +0.6 1.5 molybdopterin synthase, small subunit (NCBI) compare
b1186 nhaB +0.6 3.2 NhaB sodium/proton transporter (Katherine Huang) compare
b1660 ydhC +0.6 2.8 predicted transporter (NCBI) compare
b4594 ymgJ +0.6 1.4 hypothetical protein (RefSeq) compare
b0423 thiI +0.6 2.9 thiamine biosynthesis protein ThiI (NCBI) compare
b3789 rffH +0.6 3.9 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b2948 yqgE +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b4568 ytjA +0.6 1.2 predicted protein (RefSeq) compare
b1013 ycdC +0.6 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1515 lsrD +0.6 2.0 AI2 transporter (NCBI) compare
b2083 yegZ +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b2114 metG +0.6 0.9 methionyl-tRNA synthetase (NCBI) compare
b4325 yjiC +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b1324 tpx +0.6 2.4 thiol peroxidase (NCBI) compare
b1299 puuR +0.6 2.8 DNA-binding transcriptional repressor (NCBI) compare
b2528 iscA +0.6 1.0 iron-sulfur cluster assembly protein (NCBI) compare
b0381 ddlA +0.6 2.9 D-alanylalanine synthetase (NCBI) compare
b4431 rprA +0.6 1.3 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b0226 dinJ +0.6 2.0 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b4589 ylcI +0.6 1.1 hypothetical protein (RefSeq) compare
b1741 ydjQ +0.6 1.9 endonuclease of nucleotide excision repair (NCBI) compare
b2209 eco +0.6 1.0 ecotin precursor (NCBI) compare


Specific Phenotypes

For 6 genes in this experiment

For carbon source D-Mannose in Escherichia coli BW25113

For carbon source D-Mannose across organisms