Experiment set40IT057 for Escherichia coli BW25113

Compare to:

carbon source D-Glucose 20mM

200 most detrimental genes:

  gene name fitness t score description  
b3035 tolC +4.1 28.1 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b2904 gcvH +4.0 11.4 glycine cleavage system protein H (NCBI) compare
b2903 gcvP +3.8 34.4 glycine dehydrogenase (NCBI) compare
b2144 sanA +3.7 28.7 hypothetical protein (NCBI) compare
b2829 ptsP +3.7 25.7 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2572 rseA +3.7 6.2 anti-sigma factor (NCBI) compare
b3405 ompR +3.5 13.4 osmolarity response regulator (NCBI) compare
b3206 npr +3.4 17.8 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2905 gcvT +3.4 21.3 glycine cleavage system aminomethyltransferase T (NCBI) compare
b1830 prc +3.2 16.2 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0178 hlpA +3.2 12.1 periplasmic chaperone (NCBI) compare
b0632 dacA +3.2 19.5 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3911 cpxA +3.1 22.7 two-component sensor protein (NCBI) compare
b0437 clpP +3.1 16.6 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1677 lpp +3.1 7.4 murein lipoprotein (NCBI) compare
b3630 rfaP +3.0 23.3 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3620 rfaF +2.9 18.0 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0053 surA +2.8 18.1 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0438 clpX +2.8 14.9 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3784 rfe +2.7 24.3 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3052 rfaE +2.6 12.1 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2017 yefM +2.6 5.3 orf, hypothetical protein (VIMSS) compare
b3404 envZ +2.6 15.5 osmolarity sensor protein (NCBI) compare
b0222 lpcA +2.6 9.7 phosphoheptose isomerase (NCBI) compare
b3621 rfaC +2.6 14.6 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3631 rfaG +2.6 22.1 glucosyltransferase I (NCBI) compare
b3619 rfaD +2.6 12.7 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2064 asmA +2.5 10.9 predicted assembly protein (NCBI) compare
b3842 rfaH +2.5 15.2 transcriptional activator RfaH (NCBI) compare
b3199 yrbK +2.5 8.1 hypothetical protein (NCBI) compare
b3787 rffD +2.5 19.4 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b2951 yggS +2.5 12.2 predicted enzyme (NCBI) compare
b3786 rffE +2.4 19.4 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2808 gcvA +2.3 7.7 DNA-binding transcriptional dual regulator (NCBI) compare
b4042 dgkA +2.3 7.2 diacylglycerol kinase (NCBI) compare
b3261 fis +2.3 7.8 DNA-binding protein Fis (NCBI) compare
b3794 rffM +2.3 14.3 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b2188 yejM +2.2 10.8 predicted hydrolase, inner membrane (NCBI) compare
b3163 nlpI +2.0 7.5 hypothetical protein (NCBI) compare
b1236 galU +2.0 10.0 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1863 ruvC +2.0 5.1 Holliday junction resolvase (NCBI) compare
b1860 ruvB +2.0 2.2 Holliday junction DNA helicase B (NCBI) compare
b2742 nlpD +1.9 9.2 predicted outer membrane lipoprotein (NCBI) compare
b1861 ruvA +1.9 3.4 Holliday junction DNA helicase motor protein (NCBI) compare
b3082 ygjM +1.9 2.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b3979 thrT +1.9 1.0 tRNA-Thr (NCBI) compare
b3150 yraP +1.8 10.1 hypothetical protein (NCBI) compare
b3920 yiiQ +1.8 10.0 hypothetical protein (NCBI) compare
b4552 yrhC +1.7 2.1 no description compare
b0059 hepA +1.7 14.0 ATP-dependent helicase HepA (NCBI) compare
b2830 nudH +1.7 3.8 dinucleoside polyphosphate hydrolase (NCBI) compare
b3790 rffC +1.7 7.8 TDP-fucosamine acetyltransferase (RefSeq) compare
b4169 amiB +1.7 11.8 N-acetylmuramoyl-l-alanine amidase II (NCBI) compare
b4000 hupA +1.7 8.5 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0677 nagA +1.7 7.7 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b0232 yafN +1.6 2.2 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2175 spr +1.6 5.6 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3791 rffA +1.6 9.5 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3021 ygiT +1.6 3.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3387 dam +1.6 11.5 DNA adenine methylase (NCBI) compare
b3341 rpsG +1.6 2.0 30S ribosomal protein S7 (NCBI) compare
b2897 ygfY +1.6 4.9 hypothetical protein (NCBI) compare
b0695 kdpD +1.6 5.6 fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein (NCBI) compare
b1855 lpxM +1.5 4.6 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4458 oxyS +1.5 2.4 global regulatory RNA (NCBI) compare
b4203 rplI +1.5 7.1 50S ribosomal protein L9 (NCBI) compare
b3627 rfaI +1.5 10.1 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1765 ydjA +1.5 1.6 predicted oxidoreductase (NCBI) compare
b3164 pnp +1.5 2.8 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4481 rffT +1.4 7.2 4-alpha-L-fucosyltransferase (NCBI) compare
b3778 rep +1.4 8.4 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3529 yhjK +1.4 10.7 predicted diguanylate cyclase (RefSeq) compare
b3655 yicH +1.4 12.0 hypothetical protein (NCBI) compare
b1291 sapD +1.4 2.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3806 cyaA +1.4 6.3 adenylate cyclase (NCBI) compare
b4590 ybfK +1.4 2.8 hypothetical protein (RefSeq) compare
b3935 priA +1.3 2.1 primosome assembly protein PriA (NCBI) compare
b0092 ddlB +1.3 5.7 D-alanylalanine synthetase (NCBI) compare
b0759 galE +1.3 6.6 UDP-galactose-4-epimerase (VIMSS) compare
b4451 ryhB +1.3 2.9 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b1147 ymfL +1.2 1.6 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b3632 rfaQ +1.2 10.5 lipopolysaccharide core biosynthesis protein (NCBI) compare
b3609 secB +1.2 3.5 export protein SecB (NCBI) compare
b1575 dicB +1.2 1.4 Qin prophage; cell division inhibition protein (NCBI) compare
b3977 tyrU +1.1 1.4 tRNA-Tyr (NCBI) compare
b0440 hupB +1.1 4.0 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b4702 mgtL +1.1 1.4 regulatory leader peptide for mgtA (NCBI) compare
b2259 pmrD +1.1 1.3 polymyxin resistance protein B (VIMSS) compare
b2516 yfgA +1.1 4.9 hypothetical protein (NCBI) compare
b4100 phnH +1.1 3.8 carbon-phosphorus lyase complex subunit (NCBI) compare
b3785 wzzE +1.1 7.2 putative transport protein (VIMSS) compare
b3229 sspA +1.0 2.9 stringent starvation protein A (NCBI) compare
b4545 ypdJ +1.0 2.4 no description compare
b2688 gshA +1.0 4.5 glutamate--cysteine ligase (NCBI) compare
b1085 b1085 +1.0 2.8 orf, hypothetical protein (VIMSS) compare
b1874 cutC +1.0 1.9 copper homeostasis protein (NCBI) compare
b1273 yciN +1.0 1.9 hypothetical protein (NCBI) compare
b2494 yfgC +1.0 6.5 predicted peptidase (NCBI) compare
b2817 b2817 +1.0 3.3 putative amidase (VIMSS) compare
b2617 smpA +1.0 1.9 small membrane protein A (VIMSS) compare
b0882 clpA +0.9 5.7 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b3260 dusB +0.9 3.7 tRNA-dihydrouridine synthase B (NCBI) compare
b1143 ymfI +0.9 1.4 orf, hypothetical protein (VIMSS) compare
b4646 yrdE +0.9 2.8 no description compare
b0957 ompA +0.9 2.9 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b0434 yajG +0.9 4.7 putative polymerase/proteinase (VIMSS) compare
b3181 greA +0.9 3.1 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1164 ycgZ +0.9 1.5 hypothetical protein (NCBI) compare
b1642 slyA +0.9 1.8 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b3628 rfaB +0.9 5.6 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1111 ycfQ +0.9 4.1 orf, hypothetical protein (VIMSS) compare
b3484 yhhI +0.9 2.7 predicted transposase (NCBI) compare
b1609 rstB +0.9 3.4 sensory histidine kinase in two-component regulatory system with RstA (NCBI) compare
b1222 narX +0.9 3.1 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b1708 nlpC +0.9 3.3 predicted lipoprotein (NCBI) compare
b1620 malI +0.9 1.4 DNA-binding transcriptional repressor (NCBI) compare
b0729 sucD +0.9 3.9 succinyl-CoA synthetase subunit alpha (NCBI) compare
b0627 tatE +0.9 1.4 twin arginine translocase protein E (NCBI) compare
b3426 glpD +0.9 2.5 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b1293 sapB +0.9 1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0542 renD +0.9 2.3 orf, hypothetical protein (VIMSS) compare
b0803 ybiI +0.9 3.1 hypothetical protein (NCBI) compare
b3643 rph +0.8 3.3 ribonuclease PH (NCBI) compare
b2362 yfdS +0.8 2.7 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1936 intG +0.8 1.3 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1728 ydjM +0.8 1.6 predicted inner membrane protein regulated by LexA (RefSeq) compare
b2183 rsuA +0.8 2.5 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b1278 pgpB +0.8 2.6 phosphatidylglycerophosphatase B (NCBI) compare
b3250 mreC +0.8 1.2 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b2313 cvpA +0.8 1.3 membrane protein required for colicin V production (NCBI) compare
b3472 b3472 +0.8 4.7 orf, hypothetical protein (VIMSS) compare
b1359 ydaU +0.8 1.5 Rac prophage; conserved protein (NCBI) compare
b1518 lsrG +0.8 1.6 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b3527 yhjJ +0.8 6.8 predicted zinc-dependent peptidase (NCBI) compare
b0929 ompF +0.8 2.6 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b2963 mltC +0.8 5.9 membrane-bound lytic murein transglycosylase C (VIMSS) compare
b1606 folM +0.8 4.3 short chain dehydrogenase (NCBI) compare
b4441 glmY +0.8 1.5 sRNA activator of glmS mRNA (RefSeq) compare
b3652 recG +0.8 4.3 ATP-dependent DNA helicase (NCBI) compare
b2947 gshB +0.8 3.0 glutathione synthetase (NCBI) compare
b2288 nuoA +0.8 1.4 NADH dehydrogenase I chain A (VIMSS) compare
b0018 mokC +0.8 1.1 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b1824 yobF +0.8 2.2 hypothetical protein (NCBI) compare
b3625 rfaY +0.8 3.1 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2894 xerD +0.8 0.9 site-specific tyrosine recombinase XerD (NCBI) compare
b0082 mraW +0.8 3.6 S-adenosyl-methyltransferase (NCBI) compare
b0686 ybfF +0.8 3.4 hypothetical protein (NCBI) compare
b1075 flgD +0.8 1.8 flagellar basal body rod modification protein D (NCBI) compare
b2809 b2809 +0.8 1.8 orf, hypothetical protein (VIMSS) compare
b2597 yfiA +0.7 1.6 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b3351 kefG +0.7 3.8 glutathione-regulated potassium-efflux system ancillary protein (NCBI) compare
b3183 obgE +0.7 0.5 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1271 yciK +0.7 2.1 short chain dehydrogenase (NCBI) compare
b2052 fcl +0.7 2.4 bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI) compare
b3195 yrbF +0.7 4.4 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4438 cyaR +0.7 1.9 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b2722 hycD +0.7 3.3 hydrogenase 3, membrane subunit (NCBI) compare
b0560 nohB +0.7 2.2 DLP12 prophage; DNA packaging protein (NCBI) compare
b2420 yfeS +0.7 2.9 hypothetical protein (NCBI) compare
b1095 fabF +0.7 3.5 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1918 yecS +0.7 2.3 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2104 thiM +0.7 1.2 hydroxyethylthiazole kinase (NCBI) compare
b3149 yraO +0.7 3.4 DnaA initiator-associating factor for replication initiation (NCBI) compare
b0926 ycbK +0.7 3.5 hypothetical protein (NCBI) compare
b2209 eco +0.7 1.2 ecotin precursor (NCBI) compare
b2457 cchA +0.7 1.7 detox protein (VIMSS) compare
b4461 yfjD +0.7 3.6 predicted inner membrane protein (RefSeq) compare
b0226 dinJ +0.7 1.8 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b0633 rlpA +0.7 3.9 minor lipoprotein (NCBI) compare
b1755 ynjC +0.7 2.7 fused transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b3102 yqjG +0.7 1.5 predicted S-transferase (NCBI) compare
b3194 yrbE +0.7 4.8 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1519 tam +0.7 1.6 trans-aconitate 2-methyltransferase (NCBI) compare
b2419 yfeK +0.7 1.1 hypothetical protein (NCBI) compare
b1837 yebW +0.7 1.2 orf, hypothetical protein (VIMSS) compare
b0296 ykgM +0.7 1.5 50S ribosomal protein L31 (NCBI) compare
b0728 sucC +0.7 3.0 succinyl-CoA synthetase subunit beta (NCBI) compare
b2912 ygfA +0.7 1.6 putative ligase (VIMSS) compare
b1306 pspC +0.7 3.0 DNA-binding transcriptional activator (NCBI) compare
b4594 ymgJ +0.7 1.1 hypothetical protein (RefSeq) compare
b1097 yceG +0.6 2.4 predicted aminodeoxychorismate lyase (NCBI) compare
b1380 ldhA +0.6 1.5 D-lactate dehydrogenase (NCBI) compare
b1752 ydjZ +0.6 2.1 conserved inner membrane protein (NCBI) compare
b2134 pbpG +0.6 4.3 penicillin-binding protein 7 (VIMSS) compare
b3813 uvrD +0.6 4.9 DNA-dependent ATPase I and helicase II (NCBI) compare
b3350 kefB +0.6 5.2 glutathione-regulated potassium-efflux system protein (NCBI) compare
b1358 ydaT +0.6 0.5 Rac prophage; predicted protein (NCBI) compare
b3443 yrhA +0.6 0.8 orf, hypothetical protein (VIMSS) compare
b4543 ypaA +0.6 1.5 predicted protein (NCBI) compare
b2738 ygbL +0.6 3.0 hypothetical protein (NCBI) compare
b1659 ydhB +0.6 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3096 yqjB +0.6 2.4 hypothetical protein (NCBI) compare
b2910 zapA +0.6 2.1 protein that localizes to the cytokinetic ring (NCBI) compare
b3928 yiiU +0.6 2.5 hypothetical protein (NCBI) compare
b2236 yfaE +0.6 1.0 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b4202 rpsR +0.6 0.8 30S ribosomal protein S18 (NCBI) compare
b3836 b3836 +0.6 3.1 orf, hypothetical protein (VIMSS) compare
b4551 yheV +0.6 1.4 hypothetical protein (NCBI) compare
b0998 torD +0.6 0.7 chaperone protein TorD (NCBI) compare
b1066 rimJ +0.6 2.8 ribosomal-protein-S5-alanine N-acetyltransferase (NCBI) compare


Specific Phenotypes

For 7 genes in this experiment

For carbon source D-Glucose in Escherichia coli BW25113

For carbon source D-Glucose across organisms