Experiment set3S373 for Phocaeicola dorei CL03T12C01

D-Raffinose pentahydrate 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Raffinose pentahydrate (2.5 mg/ml)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 20-Mar-24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 2 genes in this experiment

For carbon source D-Raffinose pentahydrate in Phocaeicola dorei CL03T12C01

For carbon source D-Raffinose pentahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Fructooligosaccharides(FOS) and Raffinose Utilization	1
Galactosylceramide and Sulfatide metabolism	1
Lactose and Galactose Uptake and Utilization	1
Melibiose Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Glycerolipid metabolism
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - globo series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
melibiose degradation	1	1	1
stachyose degradation	7	4	2