Experiment set3S344 for Phocaeicola dorei CL03T12C01

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Rhamnogalacturonan from soy bean pectic fibre; autoclaved 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Rhamnogalacturonan from soy bean pectic fibre; autoclaved (2.5 mg/ml)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 7 genes in this experiment

For carbon source Rhamnogalacturonan from soy bean pectic fibre; autoclaved in Phocaeicola dorei CL03T12C01

For carbon source Rhamnogalacturonan from soy bean pectic fibre; autoclaved across organisms

SEED Subsystems

Subsystem #Specific
IbrA and IbrB: co-activators of prophage gene expression 1
Iron acquisition in Vibrio 1
L-rhamnose utilization 1
Ton and Tol transport systems 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.