Experiment set3S334 for Phocaeicola dorei CL03T12C01

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Glycogen; filtered 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Glycogen; filtered (2.5 mg/ml)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 8 genes in this experiment

For carbon source Glycogen; filtered in Phocaeicola dorei CL03T12C01

For carbon source Glycogen; filtered across organisms

SEED Subsystems

Subsystem #Specific
Adenosyl nucleosidases 1
Deoxyribose and Deoxynucleoside Catabolism 1
Murein hydrolase regulation and cell death 1
Purine conversions 1
Thiamin biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyrimidine ribonucleosides salvage I 3 3 3
pyrimidine ribonucleosides degradation 2 2 2
purine deoxyribonucleosides degradation I 4 3 3
purine ribonucleosides degradation 6 4 4
purine deoxyribonucleosides degradation II 3 2 2
pyrimidine deoxyribonucleosides degradation 3 2 2
purine nucleotides degradation II (aerobic) 11 8 6
CMP phosphorylation 2 2 1
adenine and adenosine salvage I 2 2 1
xanthine and xanthosine salvage 2 2 1
guanine and guanosine salvage I 2 2 1
inosine 5'-phosphate degradation 4 3 2
guanosine nucleotides degradation III 4 3 2
adenine and adenosine salvage III 4 3 2
pyrimidine ribonucleosides salvage II 2 1 1
superpathway of purine deoxyribonucleosides degradation 7 4 3
superpathway of pyrimidine ribonucleosides salvage 10 9 4
nucleoside and nucleotide degradation (archaea) 10 4 4
superpathway of pyrimidine ribonucleosides degradation 5 2 2
adenosine nucleotides degradation II 5 2 2
superpathway of guanine and guanosine salvage 3 2 1
NAD salvage pathway III (to nicotinamide riboside) 3 2 1
superpathway of pyrimidine deoxyribonucleosides degradation 6 3 2
adenine and adenosine salvage V 3 1 1
UTP and CTP dephosphorylation I 7 5 2
ureide biosynthesis 7 3 2
pyrimidine deoxyribonucleotide phosphorylation 4 3 1
guanosine nucleotides degradation II 4 2 1
purine nucleotides degradation I (plants) 12 5 3
adenosine nucleotides degradation I 8 3 2
guanosine nucleotides degradation I 4 1 1
superpathway of pyrimidine deoxyribonucleoside salvage 9 7 2
pyrimidine deoxyribonucleosides salvage 5 4 1
nucleoside and nucleotide degradation (halobacteria) 6 2 1
superpathway of guanosine nucleotides degradation (plants) 6 2 1
superpathway of purines degradation in plants 18 5 3
fluoroacetate and fluorothreonine biosynthesis 6 1 1
superpathway of purine nucleotide salvage 14 13 2
tunicamycin biosynthesis 9 1 1
NAD salvage (plants) 11 4 1
salinosporamide A biosynthesis 15 4 1
arsenic detoxification (mammals) 17 7 1