Experiment set3S333 for Phocaeicola dorei CL03T12C01

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Glycogen; filtered 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Glycogen; filtered (2.5 mg/ml)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source Glycogen; filtered in Phocaeicola dorei CL03T12C01

For carbon source Glycogen; filtered across organisms

SEED Subsystems

Subsystem #Specific
Adenosyl nucleosidases 1
Deoxyribose and Deoxynucleoside Catabolism 1
Purine conversions 1
Thiamin biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
purine deoxyribonucleosides degradation I 4 3 3
pyrimidine ribonucleosides salvage I 3 3 2
purine ribonucleosides degradation 6 4 4
purine deoxyribonucleosides degradation II 3 2 2
purine nucleotides degradation II (aerobic) 11 8 6
guanine and guanosine salvage I 2 2 1
CMP phosphorylation 2 2 1
pyrimidine ribonucleosides degradation 2 2 1
adenine and adenosine salvage I 2 2 1
xanthine and xanthosine salvage 2 2 1
guanosine nucleotides degradation III 4 3 2
inosine 5'-phosphate degradation 4 3 2
adenine and adenosine salvage III 4 3 2
superpathway of purine deoxyribonucleosides degradation 7 4 3
nucleoside and nucleotide degradation (archaea) 10 4 4
adenosine nucleotides degradation II 5 2 2
superpathway of guanine and guanosine salvage 3 2 1
NAD salvage pathway III (to nicotinamide riboside) 3 2 1
pyrimidine deoxyribonucleosides degradation 3 2 1
adenine and adenosine salvage V 3 1 1
superpathway of pyrimidine ribonucleosides salvage 10 9 3
UTP and CTP dephosphorylation I 7 5 2
ureide biosynthesis 7 3 2
pyrimidine deoxyribonucleotide phosphorylation 4 3 1
guanosine nucleotides degradation II 4 2 1
purine nucleotides degradation I (plants) 12 5 3
adenosine nucleotides degradation I 8 3 2
guanosine nucleotides degradation I 4 1 1
superpathway of pyrimidine ribonucleosides degradation 5 2 1
superpathway of pyrimidine deoxyribonucleosides degradation 6 3 1
nucleoside and nucleotide degradation (halobacteria) 6 2 1
superpathway of guanosine nucleotides degradation (plants) 6 2 1
superpathway of purines degradation in plants 18 5 3
fluoroacetate and fluorothreonine biosynthesis 6 1 1
superpathway of purine nucleotide salvage 14 13 2
superpathway of pyrimidine deoxyribonucleoside salvage 9 7 1
tunicamycin biosynthesis 9 1 1
NAD salvage (plants) 11 4 1
salinosporamide A biosynthesis 15 4 1
arsenic detoxification (mammals) 17 7 1