Experiment set3S310 for Escherichia coli ECOR27

Compare to:

Bas31

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 5/14/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 24 genes in this experiment

For phage Phage in Escherichia coli ECOR27

For phage Phage across organisms

SEED Subsystems

Subsystem #Specific
Flagellum 2
Biogenesis of c-type cytochromes 1
D-Galacturonate and D-Glucuronate Utilization 1
DNA-replication 1
Orphan regulatory proteins 1
Periplasmic disulfide interchange 1
Terminal cytochrome O ubiquinol oxidase 1
Terminal cytochrome oxidases 1
Ton and Tol transport systems 1
Type III secretion system orphans 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-serine degradation 3 3 3
L-tryptophan degradation II (via pyruvate) 3 3 2
D-serine degradation 3 3 2
L-cysteine degradation II 3 2 2
siroheme biosynthesis 4 4 2
periplasmic disulfide bond reduction 2 2 1
glycine betaine degradation III 7 4 3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
glycine betaine degradation I 8 4 3
glycine degradation 3 3 1
L-methionine biosynthesis II 6 5 2
pectin degradation I 3 1 1
factor 430 biosynthesis 7 3 2
L-mimosine degradation 8 4 2
glutathione-mediated detoxification I 8 3 2
pectin degradation II 5 3 1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 2 2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 3 2
purine nucleobases degradation II (anaerobic) 24 17 3
cytochrome c biogenesis (system I type) 11 11 1
adenosylcobalamin biosynthesis II (aerobic) 33 17 2
adenosylcobalamin biosynthesis I (anaerobic) 36 16 2