Experiment set3S310 for Escherichia coli ECOR27
Bas31
Group: phageMedia: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 5/14/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 16 genes in this experiment
For phage Phage in Escherichia coli ECOR27
For phage Phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Flagellum | 1 |
| Orphan regulatory proteins | 1 |
| Terminal cytochrome O ubiquinol oxidase | 1 |
| Terminal cytochrome oxidases | 1 |
| Type III secretion system orphans | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| siroheme biosynthesis | 4 | 4 | 2 |
| factor 430 biosynthesis | 7 | 3 | 2 |
| cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) | 13 | 2 | 2 |
| cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) | 15 | 3 | 2 |
| adenosylcobalamin biosynthesis II (aerobic) | 33 | 17 | 2 |
| adenosylcobalamin biosynthesis I (anaerobic) | 36 | 16 | 2 |