Experiment set3S309 for Escherichia coli ECOR27

Bas31

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 5/14/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 11 genes in this experiment

For phage Phage in Escherichia coli ECOR27

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Flagellum	2
DNA-replication	1
DNA repair, UvrABC system	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Peptidyl-prolyl cis-trans isomerase	1
Potassium homeostasis	1
Terminal cytochrome O ubiquinol oxidase	1
Terminal cytochrome oxidases	1
Type III secretion system orphans	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Folate biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NiFe(CO)(CN)2 cofactor biosynthesis	10	9	1