Experiment set3S294 for Phocaeicola dorei CL03T12C01

Red Arabinan from sugar-beet; filtered 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Red Arabinan from sugar-beet; filtered (2.5 mg/ml)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 7 genes in this experiment

For carbon source Red Arabinan from sugar-beet; filtered in Phocaeicola dorei CL03T12C01

For carbon source Red Arabinan from sugar-beet; filtered across organisms

SEED Subsystems

Subsystem	#Specific
Alkylphosphonate utilization	1
L-Arabinose utilization	1
Xylose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Starch and sucrose metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
(1,4)-β-D-xylan degradation	2	2	1