Experiment set3S110 for Escherichia coli ECRC100

EV240

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECRC100_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 5/7/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 2 genes in this experiment

For phage Phage in Escherichia coli ECRC100

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Ribosome biogenesis bacterial	1
Type III secretion system orphans	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pseudouridine degradation	2	2	1