Experiment set3IT091 for Pseudomonas fluorescens GW456-L13

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N-Acetyl-D-Glucosamine nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + N-Acetyl-D-Glucosamine (20 mM), pH=7
Culturing: pseudo13_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Mark on 1/21/2016
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 8 genes in this experiment

For nitrogen source N-Acetyl-D-Glucosamine in Pseudomonas fluorescens GW456-L13

For nitrogen source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 3
Sialic Acid Metabolism 2
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 2
Fructose and Mannose Inducible PTS 1
Fructose utilization 1
Mannitol Utilization 1
Murein hydrolase regulation and cell death 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 2
N-acetylglucosamine degradation II 3 2 2
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 2 2
D-galactosamine and N-acetyl-D-galactosamine degradation 4 2 1
chitin derivatives degradation 8 2 2
N-acetyl-D-galactosamine degradation 5 2 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
chitin degradation I (archaea) 7 1 1
glycolysis IV 10 8 1
superpathway of N-acetylneuraminate degradation 22 15 2
peptidoglycan recycling I 14 12 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 17 1