Experiment set3IT087 for Agrobacterium fabrum C58

Nicotinamide nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Succinate_noNitrogen + Nicotinamide (5 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 11/20/20
Media components: 10 mM Sodium succinate dibasic hexahydrate, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 1 genes in this experiment

For nitrogen source Nicotinamide in Agrobacterium fabrum C58

For nitrogen source Nicotinamide across organisms

SEED Subsystems

Subsystem	#Specific
NAD and NADP cofactor biosynthesis global	1
NAD regulation	1
Redox-dependent regulation of nucleus processes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nicotinate and nicotinamide metabolism
• Lysine biosynthesis
• Glutathione metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Sphingolipid metabolism
• 1,4-Dichlorobenzene degradation
• Caprolactam degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
aldoxime degradation	3	1	1
NAD salvage pathway V (PNC V cycle)	5	4	1
NAD salvage pathway I (PNC VI cycle)	7	6	1
NAD salvage (plants)	11	6	1
superpathway of NAD biosynthesis in eukaryotes	14	6	1