Experiment set3IT081 for Agrobacterium fabrum C58

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5-Aminolevulinic acid nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Succinate_noNitrogen + 5-Aminolevulinic acid (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 11/20/20
Media components: 10 mM Sodium succinate dibasic hexahydrate, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 4 genes in this experiment

For nitrogen source 5-Aminolevulinic acid in Agrobacterium fabrum C58

For nitrogen source 5-Aminolevulinic acid across organisms

SEED Subsystems

Subsystem #Specific
Lipid A modifications 1
Nitrate and nitrite ammonification 1
Orphan regulatory proteins 1
Pyruvate Alanine Serine Interconversions 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
β-alanine degradation II 2 2 1
β-alanine biosynthesis II 6 3 1
superpathway of coenzyme A biosynthesis II (plants) 10 7 1