Experiment set3IT080 for Pseudomonas fluorescens GW456-L13

Compare to:

L-Isoleucine nitrogen source

200 most important genes:

  gene name fitness t score description  
PfGW456L13_358 -6.4 -4.4 Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-) compare
PfGW456L13_3948 -6.1 -4.2 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
PfGW456L13_149 -6.0 -5.9 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
PfGW456L13_303 -6.0 -4.1 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
PfGW456L13_347 -5.8 -8.0 Nitrogen regulation protein NR(I) compare
PfGW456L13_2046 -5.8 -4.0 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94) compare
PfGW456L13_1368 -5.7 -3.9 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
PfGW456L13_3934 -5.7 -10.8 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
PfGW456L13_3945 -5.5 -7.6 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
PfGW456L13_172 -5.5 -9.2 Phosphoserine phosphatase (EC 3.1.3.3) compare
PfGW456L13_1540 -5.3 -10.9 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
PfGW456L13_3947 -5.2 -5.1 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
PfGW456L13_5000 -5.1 -15.1 Histidinol dehydrogenase (EC 1.1.1.23) compare
PfGW456L13_913 -5.1 -6.1 N-acetylglutamate synthase (EC 2.3.1.1) compare
PfGW456L13_1222 -5.1 -7.0 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
PfGW456L13_1217 -5.1 -22.3 Anthranilate synthase, aminase component (EC 4.1.3.27) compare
PfGW456L13_361 -5.0 -14.1 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
PfGW456L13_1233 -5.0 -3.4 N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) compare
PfGW456L13_4854 -4.9 -17.9 2-isopropylmalate synthase (EC 2.3.3.13) compare
PfGW456L13_362 -4.8 -9.2 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
PfGW456L13_3940 -4.8 -10.2 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
PfGW456L13_5001 -4.8 -11.9 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
PfGW456L13_2032 -4.7 -4.6 Cysteine synthase B (EC 2.5.1.47) compare
PfGW456L13_854 -4.7 -3.2 Argininosuccinate lyase (EC 4.3.2.1) compare
PfGW456L13_845 -4.7 -7.1 Diaminopimelate decarboxylase (EC 4.1.1.20) compare
PfGW456L13_951 -4.6 -14.6 Phosphoserine phosphatase (EC 3.1.3.3) compare
PfGW456L13_1007 -4.6 -12.2 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
PfGW456L13_1739 -4.5 -11.7 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
PfGW456L13_1749 -4.5 -4.4 Argininosuccinate synthase (EC 6.3.4.5) compare
PfGW456L13_727 -4.5 -11.6 LysR family transcriptional regulator PA5437 compare
PfGW456L13_4009 -4.5 -13.5 Transaldolase (EC 2.2.1.2) compare
PfGW456L13_4711 -4.5 -22.9 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
PfGW456L13_359 -4.4 -10.2 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
PfGW456L13_1221 -4.4 -13.4 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
PfGW456L13_1010 -4.4 -9.7 Pyrroline-5-carboxylate reductase (EC 1.5.1.2) compare
PfGW456L13_565 -4.3 -7.7 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
PfGW456L13_302 -4.2 -7.5 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
PfGW456L13_1050 -4.1 -10.6 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
PfGW456L13_1539 -4.1 -12.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
PfGW456L13_2842 -4.1 -6.8 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
PfGW456L13_2176 -4.1 -7.1 Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51) compare
PfGW456L13_728 -4.0 -15.3 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
PfGW456L13_1538 -4.0 -13.9 Acetolactate synthase large subunit (EC 2.2.1.6) compare
PfGW456L13_1006 -3.9 -5.5 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
PfGW456L13_4125 -3.9 -2.7 Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10) compare
PfGW456L13_4115 -3.9 -7.0 2-methylaconitate cis-trans isomerase compare
PfGW456L13_566 -3.8 -9.2 Tryptophan synthase beta chain (EC 4.2.1.20) compare
PfGW456L13_793 -3.7 -3.6 Acetylglutamate kinase (EC 2.7.2.8) compare
PfGW456L13_1524 -3.7 -7.7 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
PfGW456L13_3544 -3.6 -8.9 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
PfGW456L13_1198 -3.6 -5.6 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
PfGW456L13_4116 -3.5 -14.0 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79) compare
PfGW456L13_1615 -3.5 -2.9 Probable transmembrane protein compare
PfGW456L13_943 -3.5 -7.5 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
PfGW456L13_1220 -3.4 -6.1 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
PfGW456L13_2656 -3.4 -8.3 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
PfGW456L13_1735 -3.4 -3.3 Glycerol uptake facilitator protein compare
PfGW456L13_2843 -3.4 -7.3 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
PfGW456L13_2981 -3.3 -2.5 Probable acyl-CoA dehydrogenase (EC 1.3.99.3) compare
PfGW456L13_2230 -3.3 -9.3 Cys regulon transcriptional activator CysB compare
PfGW456L13_1391 -3.3 -3.5 Transcriptional regulator, AsnC family compare
PfGW456L13_729 -3.3 -11.0 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
PfGW456L13_3253 -3.3 -14.6 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) compare
PfGW456L13_4976 -3.2 -4.8 putative membrane protein compare
PfGW456L13_4118 -3.1 -9.5 Methylisocitrate lyase (EC 4.1.3.30) compare
PfGW456L13_4671 -3.1 -10.7 Transcriptional regulator, Cro/CI family compare
PfGW456L13_1147 -3.1 -12.2 Biotin synthase (EC 2.8.1.6) compare
PfGW456L13_1521 -3.0 -4.0 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
PfGW456L13_4165 -2.9 -4.4 Chorismate synthase (EC 4.2.3.5) compare
PfGW456L13_4253 -2.9 -5.1 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
PfGW456L13_1889 -2.9 -4.3 Phosphogluconate dehydratase (EC 4.2.1.12) compare
PfGW456L13_1472 -2.9 -6.0 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
PfGW456L13_150 -2.8 -3.1 hypothetical protein compare
PfGW456L13_1813 -2.8 -8.3 Pyruvate kinase (EC 2.7.1.40) compare
PfGW456L13_4088 -2.8 -4.1 Transcriptional regulator, GntR family compare
PfGW456L13_726 -2.8 -7.0 Phosphogluconate repressor HexR, RpiR family compare
PfGW456L13_2103 -2.7 -4.4 Phosphate:acyl-ACP acyltransferase PlsX compare
PfGW456L13_2984 -2.7 -4.8 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
PfGW456L13_1150 -2.7 -13.2 Biotin synthesis protein BioC compare
PfGW456L13_3960 -2.6 -9.7 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
PfGW456L13_644 -2.5 -8.0 Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) compare
PfGW456L13_276 -2.5 -2.2 Shikimate kinase I (EC 2.7.1.71) compare
PfGW456L13_2982 -2.4 -5.0 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (from data) conserved
PfGW456L13_1044 -2.4 -10.7 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
PfGW456L13_2035 -2.4 -8.7 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
PfGW456L13_275 -2.4 -2.8 3-dehydroquinate synthase (EC 4.2.3.4) compare
PfGW456L13_2186 -2.4 -6.0 UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.-) compare
PfGW456L13_874 -2.4 -16.5 Polyphosphate kinase (EC 2.7.4.1) compare
PfGW456L13_3540 -2.3 -7.8 branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data) conserved
PfGW456L13_1151 -2.3 -7.0 Dethiobiotin synthetase (EC 6.3.3.3) compare
PfGW456L13_1148 -2.2 -13.2 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
PfGW456L13_2551 -2.1 -4.1 Cell division trigger factor (EC 5.2.1.8) compare
PfGW456L13_1838 -2.1 -5.2 Sensory box histidine kinase compare
PfGW456L13_4859 -2.1 -4.1 Outer membrane protein YfgL, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and NlpB) compare
PfGW456L13_973 -2.1 -10.5 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
PfGW456L13_360 -2.0 -3.3 FIG00956267: hypothetical protein compare
PfGW456L13_3238 -2.0 -7.8 Transcriptional regulator, AraC family conserved
PfGW456L13_2189 -2.0 -7.4 UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid transferase compare
PfGW456L13_2931 -2.0 -1.9 transcriptional regulator MvaT, P16 subunit, putative compare
PfGW456L13_403 -2.0 -3.9 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
PfGW456L13_50 -1.9 -6.3 3-dehydroquinate dehydratase II (EC 4.2.1.10) compare
PfGW456L13_1205 -1.9 -8.1 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
PfGW456L13_3541 -1.9 -9.4 branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit (EC 1.2.4.4) (from data) conserved
PfGW456L13_3542 -1.9 -7.1 Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) conserved
PfGW456L13_5150 -1.9 -2.9 Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) compare
PfGW456L13_1498 -1.9 -1.9 FIG060329: MOSC domain protein compare
PfGW456L13_1985 -1.8 -4.3 Alginate biosynthesis transcriptional activator compare
PfGW456L13_5067 -1.8 -8.3 Ornithine decarboxylase (EC 4.1.1.17) / Arginine decarboxylase (EC 4.1.1.19) compare
PfGW456L13_4036 -1.8 -2.1 FIG00954079: hypothetical protein compare
PfGW456L13_346 -1.8 -3.1 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
PfGW456L13_1029 -1.7 -1.1 Triphosphoribosyl-dephospho-CoA synthetase (EC 2.7.8.25) compare
PfGW456L13_2237 -1.7 -2.6 Organic hydroperoxide resistance transcriptional regulator compare
PfGW456L13_1901 -1.7 -5.7 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
PfGW456L13_4170 -1.7 -4.3 Isochorismatase (EC 3.3.2.1) compare
PfGW456L13_3539 -1.7 -5.3 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
PfGW456L13_4038 -1.7 -2.5 DNA topoisomerase I (EC 5.99.1.2) compare
PfGW456L13_2552 -1.6 -6.2 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
PfGW456L13_1537 -1.6 -4.1 Hypothetical protein YqcC (clustered with tRNA pseudouridine synthase C) compare
PfGW456L13_656 -1.6 -3.4 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
PfGW456L13_5008 -1.6 -2.3 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
PfGW456L13_1899 -1.6 -2.6 Aldose 1-epimerase compare
PfGW456L13_4999 -1.6 -7.6 Histidinol-phosphate aminotransferase (EC 2.6.1.9) compare
PfGW456L13_1574 -1.5 -3.7 GTP-binding and nucleic acid-binding protein YchF compare
PfGW456L13_4033 -1.5 -1.6 Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family compare
PfGW456L13_705 -1.5 -1.4 FIG00953800: hypothetical protein compare
PfGW456L13_1628 -1.5 -5.8 ATPase, AFG1 family compare
PfGW456L13_1927 -1.5 -6.3 Gluconate permease compare
PfGW456L13_1520 -1.4 -3.8 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
PfGW456L13_852 -1.4 -1.6 FIG00954215: hypothetical protein compare
PfGW456L13_2436 -1.4 -2.7 Phenylacetic acid degradation operon negative regulatory protein PaaX compare
PfGW456L13_2983 -1.4 -7.6 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) conserved
PfGW456L13_3894 -1.4 -2.2 Integration host factor alpha subunit compare
PfGW456L13_3795 -1.4 -5.9 sensor histidine kinase compare
PfGW456L13_3543 -1.4 -5.5 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) conserved
PfGW456L13_4012 -1.3 -2.1 VacJ-like lipoprotein precursor compare
PfGW456L13_2651 -1.3 -3.6 Cell division protein FtsK compare
PfGW456L13_2170 -1.3 -2.2 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
PfGW456L13_2142 -1.3 -9.1 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) compare
PfGW456L13_3339 -1.3 -6.3 FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-) compare
PfGW456L13_4662 -1.3 -2.1 Transcriptional regulator, LuxR family compare
PfGW456L13_2581 -1.3 -2.9 Transmembrane regulator protein PrtR compare
PfGW456L13_2012 -1.3 -7.3 Sensor protein PhoQ (EC 2.7.13.3) compare
PfGW456L13_1972 -1.2 -1.5 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
PfGW456L13_1824 -1.2 -2.2 transcriptional regulator MvaT, P16 subunit compare
PfGW456L13_2717 -1.2 -3.1 hypothetical protein compare
PfGW456L13_3862 -1.2 -2.4 DoxX family protein compare
PfGW456L13_4207 -1.2 -4.5 Periplasmic protease compare
PfGW456L13_1525 -1.2 -5.8 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
PfGW456L13_1219 -1.2 -2.6 hypothetical protein compare
PfGW456L13_1655 -1.1 -1.7 D-alanine--D-alanine ligase (EC 6.3.2.4) compare
PfGW456L13_5112 -1.1 -1.6 tmRNA-binding protein SmpB compare
PfGW456L13_3704 -1.1 -2.1 Transcriptional regulator RpiR in protein degradation cluster compare
PfGW456L13_3315 -1.1 -2.4 FIG00955242: hypothetical protein compare
PfGW456L13_5005 -1.1 -3.3 Uncharacterized ABC transporter, auxiliary component YrbC compare
PfGW456L13_59 -1.1 -4.7 Sensor histidine kinase/response regulator compare
PfGW456L13_2107 -1.1 -3.4 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179) compare
PfGW456L13_3990 -1.1 -3.9 Response regulator receiver domain protein (CheY) compare
PfGW456L13_5017 -1.1 -2.7 Hypothetical ATP-binding protein UPF0042, contains P-loop compare
PfGW456L13_2553 -1.0 -5.1 ATP-dependent Clp protease ATP-binding subunit ClpX compare
PfGW456L13_1284 -1.0 -1.6 Putative transport protein compare
PfGW456L13_527 -1.0 -2.7 Transcriptional regulator compare
PfGW456L13_5121 -1.0 -1.2 hypothetical protein compare
PfGW456L13_3999 -1.0 -3.5 N-acetylmannosaminyltransferase (EC 2.4.1.187) compare
PfGW456L13_795 -1.0 -1.2 hypothetical protein compare
PfGW456L13_4842 -1.0 -2.1 Translation elongation factor LepA compare
PfGW456L13_757 -1.0 -2.3 Phosphate transport system regulatory protein PhoU compare
PfGW456L13_3291 -1.0 -1.4 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein compare
PfGW456L13_3305 -1.0 -4.2 BatD compare
PfGW456L13_5029 -0.9 -5.3 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
PfGW456L13_3996 -0.9 -5.1 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
PfGW456L13_1850 -0.9 -3.8 FIG000859: hypothetical protein YebC compare
PfGW456L13_1023 -0.9 -2.8 twitching motility protein PilH compare
PfGW456L13_1011 -0.9 -2.0 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
PfGW456L13_124 -0.9 -6.3 Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1) compare
PfGW456L13_3875 -0.9 -3.1 PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein compare
PfGW456L13_892 -0.9 -6.0 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) compare
PfGW456L13_17 -0.9 -2.3 Transcriptional regulator, ArsR family compare
PfGW456L13_784 -0.9 -4.1 Ribonuclease PH (EC 2.7.7.56) compare
PfGW456L13_274 -0.9 -5.7 DamX, an inner membrane protein involved in bile resistance compare
PfGW456L13_3808 -0.9 -2.3 Predicted transcriptional regulator of NADH dehydrogenase, Rrf2 family compare
PfGW456L13_4883 -0.9 -2.6 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
PfGW456L13_4545 -0.9 -1.1 Integral membrane protein compare
PfGW456L13_1553 -0.9 -4.7 Carbonic anhydrase (EC 4.2.1.1) compare
PfGW456L13_120 -0.9 -3.7 Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1) compare
PfGW456L13_1847 -0.9 -1.1 Glycerate kinase (EC 2.7.1.31) compare
PfGW456L13_355 -0.9 -2.4 Carboxyl-terminal protease (EC 3.4.21.102) compare
PfGW456L13_1206 -0.9 -3.5 DnaJ-like protein DjlA compare
PfGW456L13_2250 -0.8 -4.8 Probable protease htpX homolog (EC 3.4.24.-) compare
PfGW456L13_3444 -0.8 -2.4 Transcriptional regulator, TetR family compare
PfGW456L13_1580 -0.8 -4.0 FIG140336: TPR domain protein compare
PfGW456L13_1888 -0.8 -5.8 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
PfGW456L13_838 -0.8 -5.8 Ammonium transporter compare
PfGW456L13_4148 -0.8 -2.3 hypothetical protein compare
PfGW456L13_4715 -0.8 -2.0 FIG138056: a glutathione-dependent thiol reductase compare
PfGW456L13_783 -0.8 -1.9 Protein YicC compare
PfGW456L13_2620 -0.8 -1.1 Glutamine amidotransferase class-I (EC 6.3.5.2) compare
PfGW456L13_3427 -0.8 -2.2 Enoyl-CoA hydratase (EC 4.2.1.17) compare
PfGW456L13_4836 -0.8 -3.9 Transglycosylase, Slt family compare
PfGW456L13_3995 -0.8 -3.7 UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14) compare
PfGW456L13_1140 -0.8 -1.3 Glycine cleavage system transcriptional antiactivator GcvR compare


Specific Phenotypes

For 8 genes in this experiment

For nitrogen source L-Isoleucine in Pseudomonas fluorescens GW456-L13

For nitrogen source L-Isoleucine across organisms