Experiment set3IT076 for Pseudomonas sp. RS175
caffeic acid carbon source 2.5 mM
Group: carbon sourceMedia: MME_noCarbon + caffeic acid (2.5 mM), pH=7
Culturing: Pseudomonas_RS175_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Joshua Elmore on 1-July-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 11 genes in this experiment
For carbon source caffeic acid in Pseudomonas sp. RS175
For carbon source caffeic acid across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| arsenite to oxygen electron transfer | 2 | 1 | 1 |
| arsenite to oxygen electron transfer (via azurin) | 3 | 1 | 1 |
| L-histidine degradation I | 4 | 4 | 1 |
| aerobic respiration II (cytochrome c) (yeast) | 4 | 3 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 3 | 1 |
| L-histidine degradation II | 5 | 5 | 1 |
| L-histidine degradation III | 6 | 4 | 1 |
| Fe(II) oxidation | 6 | 3 | 1 |
| L-histidine degradation VI | 8 | 7 | 1 |