Experiment set3IT070 for Kangiella aquimarina DSM 16071

Compare to:

marine broth with Sodium acetate 300 mM

Group: stress
Media: marine_broth_2216 + Sodium acetate (300 mM)
Culturing: Kang_ML4, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 1/20/2015
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1097 C1,C2

Specific Phenotypes

For 4 genes in this experiment

For stress Sodium acetate in Kangiella aquimarina DSM 16071

For stress Sodium acetate across organisms

SEED Subsystems

Subsystem #Specific
Entner-Doudoroff Pathway 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
anaerobic energy metabolism (invertebrates, cytosol) 7 4 1
Entner-Doudoroff pathway III (semi-phosphorylative) 9 4 1
Entner-Doudoroff pathway I 9 4 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 3 1
glycolysis IV 10 8 1
Rubisco shunt 10 7 1
glycolysis V (Pyrococcus) 10 6 1
glycolysis II (from fructose 6-phosphate) 11 9 1
glycolysis III (from glucose) 11 8 1
glycolysis VI (from fructose) 11 7 1
homolactic fermentation 12 8 1
glycolysis I (from glucose 6-phosphate) 13 9 1
Bifidobacterium shunt 15 11 1
glycerol degradation to butanol 16 9 1
mixed acid fermentation 16 9 1
superpathway of glucose and xylose degradation 17 9 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 9 1
superpathway of anaerobic energy metabolism (invertebrates) 17 9 1
superpathway of hexitol degradation (bacteria) 18 11 1
heterolactic fermentation 18 7 1
superpathway of anaerobic sucrose degradation 19 11 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 11 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 19 1
superpathway of N-acetylneuraminate degradation 22 11 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 19 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 14 1
1-butanol autotrophic biosynthesis (engineered) 27 16 1