Experiment set3IT069 for Pseudomonas fluorescens GW456-L13
L-Lysine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + L-Lysine (20 mM), pH=7
Culturing: pseudo13_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Mark on 1/21/2016
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 22 genes in this experiment
For nitrogen source L-Lysine in Pseudomonas fluorescens GW456-L13
For nitrogen source L-Lysine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Arginine and Ornithine Degradation | 2 |
| Coenzyme PQQ synthesis | 2 |
| Lysine degradation | 2 |
| Pyrroloquinoline Quinone biosynthesis | 2 |
| Orphan regulatory proteins | 1 |
| Sulfur oxidation | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Lysine degradation
- Tryptophan metabolism
- Fatty acid metabolism
- Valine, leucine and isoleucine degradation
- Geraniol degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Biosynthesis of plant hormones
- Fatty acid elongation in mitochondria
- Synthesis and degradation of ketone bodies
- Urea cycle and metabolism of amino groups
- Glutamate metabolism
- Lysine biosynthesis
- Arginine and proline metabolism
- Tyrosine metabolism
- Benzoate degradation via hydroxylation
- alpha-Linolenic acid metabolism
- Pyruvate metabolism
- 1- and 2-Methylnaphthalene degradation
- Naphthalene and anthracene degradation
- Propanoate metabolism
- Ethylbenzene degradation
- Terpenoid biosynthesis
- Limonene and pinene degradation
- Biosynthesis of unsaturated fatty acids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: