Experiment set3IT066 for Pseudomonas sp. RS175

p-Coumaric acid carbon source 2.5 mM

Group: carbon source
Media: MME_noCarbon + p-Coumaric acid (2.5 mM), pH=7
Culturing: Pseudomonas_RS175_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Joshua Elmore on 1-July-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 18 genes in this experiment

For carbon source p-Coumaric acid in Pseudomonas sp. RS175

For carbon source p-Coumaric acid across organisms

SEED Subsystems

Subsystem	#Specific
Histidine Degradation	1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	1
Oxidative stress	1
dTDP-rhamnose synthesis	1
p-Hydroxybenzoate degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Histidine metabolism
• Benzoate degradation via hydroxylation
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• 2,4-Dichlorobenzoate degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	3	1
L-histidine degradation I	4	4	1
4-chlorobenzoate degradation	4	2	1
4-methylphenol degradation to protocatechuate	4	1	1
L-histidine degradation II	5	5	1
CDP-6-deoxy-D-gulose biosynthesis	5	3	1
4-coumarate degradation (aerobic)	5	2	1
bisphenol A degradation	5	1	1
L-histidine degradation III	6	4	1
4-hydroxymandelate degradation	6	2	1
spongiadioxin C biosynthesis	7	2	1
L-histidine degradation VI	8	7	1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	3	1
polybrominated dihydroxylated diphenyl ethers biosynthesis	8	2	1
toluene degradation III (aerobic) (via p-cresol)	11	7	1
streptomycin biosynthesis	18	3	1
superpathway of aerobic toluene degradation	30	11	1
superpathway of aromatic compound degradation via 3-oxoadipate	35	13	1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate	42	10	1