Experiment set3IT065 for Kangiella aquimarina DSM 16071

marine broth with Choline chloride 15 mM

Group: stress
Media: marine_broth_2216 + Choline chloride (15 mM)
Culturing: Kang_ML4, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 1/20/2015
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1097 A3,A4

Specific Phenotypes

For 28 genes in this experiment

For stress Choline chloride in Kangiella aquimarina DSM 16071

For stress Choline chloride across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance Efflux Pumps	2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	2
Bacterial Cell Division	1
Bacterial Chemotaxis	1
Chitin and N-acetylglucosamine utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Methylcitrate cycle	1
Propionate-CoA to Succinate Module	1
Queuosine-Archaeosine Biosynthesis	1
Type IV pilus	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Nucleotide sugars metabolism
• Biosynthesis of unsaturated fatty acids
• Tyrosine metabolism
• Aminosugars metabolism
• Glycerophospholipid metabolism
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Limonene and pinene degradation
• Biosynthesis of type II polyketide backbone
• Glycolysis / Gluconeogenesis
• Fructose and mannose metabolism
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Purine metabolism
• Valine, leucine and isoleucine degradation
• Lysine degradation
• Histidine metabolism
• Phenylalanine metabolism
• Benzoate degradation via hydroxylation
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Glutathione metabolism
• Starch and sucrose metabolism
• Lipopolysaccharide biosynthesis
• Glycerolipid metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Propanoate metabolism
• Ethylbenzene degradation
• Porphyrin and chlorophyll metabolism
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glutathione degradation (DUG pathway)	2	2	1
palmitoleate biosynthesis IV (fungi and animals)	2	2	1
(5Z)-dodecenoate biosynthesis I	6	5	3
oleate biosynthesis II (animals and fungi)	2	1	1
(5Z)-dodecenoate biosynthesis II	6	4	2
oleate biosynthesis III (cyanobacteria)	3	2	1
superpathway of unsaturated fatty acids biosynthesis (E. coli)	20	16	6
oleate biosynthesis IV (anaerobic)	14	10	4
CDP-diacylglycerol biosynthesis I	4	4	1
CDP-diacylglycerol biosynthesis II	4	4	1
superpathway of fatty acid biosynthesis I (E. coli)	16	12	4
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast)	12	9	3
gondoate biosynthesis (anaerobic)	4	3	1
palmitate biosynthesis III	29	21	7
tetradecanoate biosynthesis (mitochondria)	25	17	6
superpathway of fatty acids biosynthesis (E. coli)	53	40	12
palmitate biosynthesis II (type II fatty acid synthase)	31	22	7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	9	7	2
2-methylcitrate cycle I	5	5	1
cis-vaccenate biosynthesis	5	4	1
phosphatidate biosynthesis (yeast)	5	3	1
8-amino-7-oxononanoate biosynthesis IV	5	3	1
fatty acid elongation -- saturated	5	3	1
superpathway of fatty acid biosynthesis II (plant)	43	30	8
8-amino-7-oxononanoate biosynthesis I	11	8	2
odd iso-branched-chain fatty acid biosynthesis	34	17	6
even iso-branched-chain fatty acid biosynthesis	34	17	6
anteiso-branched-chain fatty acid biosynthesis	34	17	6
phosphatidylglycerol biosynthesis I	6	6	1
phosphatidylglycerol biosynthesis II	6	6	1
superpathway of phospholipid biosynthesis III (E. coli)	12	10	2
γ-glutamyl cycle	6	5	1
2-methylcitrate cycle II	6	5	1
L-leucine degradation I	6	4	1
stearate biosynthesis II (bacteria and plants)	6	4	1
stearate biosynthesis IV	6	4	1
chitin degradation II (Vibrio)	6	3	1
superpathway of stearidonate biosynthesis (cyanobacteria)	6	2	1
palmitoyl ethanolamide biosynthesis	6	2	1
streptorubin B biosynthesis	34	15	5
chitin degradation III (Serratia)	7	3	1
diacylglycerol and triacylglycerol biosynthesis	7	3	1
stigma estolide biosynthesis	7	2	1
chitin degradation I (archaea)	7	1	1
biotin biosynthesis I	15	12	2
anandamide biosynthesis II	8	2	1
2-allylmalonyl-CoA biosynthesis	8	2	1
sorgoleone biosynthesis	8	2	1
anandamide biosynthesis I	12	4	1
superpathway of cardiolipin biosynthesis (bacteria)	13	10	1
superpathway of phospholipid biosynthesis II (plants)	28	11	2
mycolate biosynthesis	205	16	4
superpathway of mycolate biosynthesis	239	17	4