Experiment set3IT063 for Pseudomonas fluorescens GW456-L13

LB with Lomefloxacin hydrochloride 0.0004 mM

Group: stress
Media: LB + Lomefloxacin hydrochloride (4e-04 mM)
Culturing: pseudo13_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/11/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1192 C3,C4

Specific Phenotypes

For 28 genes in this experiment

For stress Lomefloxacin hydrochloride in Pseudomonas fluorescens GW456-L13

For stress Lomefloxacin hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	2
DNA repair, bacterial	2
Multidrug Resistance Efflux Pumps	2
Queuosine-Archaeosine Biosynthesis	2
Ribosome biogenesis bacterial	2
Alginate metabolism	1
Arginine and Ornithine Degradation	1
Bacterial RNA-metabolizing Zn-dependent hydrolases	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
DNA-replication	1
DNA repair, UvrABC system	1
DNA repair, bacterial DinG and relatives	1
DNA repair, bacterial UvrD and related helicases	1
Heat shock dnaK gene cluster extended	1
Heme and Siroheme Biosynthesis	1
Lipid A modifications	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
Peptidoglycan Biosynthesis	1
Purine conversions	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Diterpenoid biosynthesis
• Ascorbate and aldarate metabolism
• Fatty acid biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Glycine, serine and threonine metabolism
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Benzoxazinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Carotenoid biosynthesis - General
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Drug metabolism - other enzymes
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
guanine and guanosine salvage I	2	2	1
guanine and guanosine salvage II	2	2	1
xanthine and xanthosine salvage	2	2	1
pseudouridine degradation	2	1	1
adenine salvage	3	2	1
superpathway of guanine and guanosine salvage	3	2	1
adenine and adenosine salvage III	4	4	1
superpathway of purine nucleotide salvage	14	13	1