Experiment set3IT058 for Pseudomonas fluorescens GW456-L13
LB with Vancomycin Hydrochloride Hydrate 0.5 mg/ml
Group: stressMedia: LB + Vancomycin Hydrochloride Hydrate (0.5 mg/ml)
Culturing: pseudo13_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/11/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1192 A5,A6
Specific Phenotypes
For 12 genes in this experiment
For stress Vancomycin Hydrochloride Hydrate in Pseudomonas fluorescens GW456-L13
For stress Vancomycin Hydrochloride Hydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| ABC transporter alkylphosphonate (TC 3.A.1.9.1) | 3 |
| DNA repair, bacterial UvrD and related helicases | 1 |
| Peptidoglycan Biosynthesis | 1 |
| Type IV pilus | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Peptidoglycan biosynthesis
- Ascorbate and aldarate metabolism
- Ubiquinone and menaquinone biosynthesis
- Lysine degradation
- Nucleotide sugars metabolism
- Glycosaminoglycan degradation
- Biotin metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 2 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 2 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 11 | 2 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 12 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 4 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 11 | 1 |