Experiment set3IT057 for Echinicola vietnamensis KMM 6221, DSM 17526

Compare to:

L-Glutamine nitrogen source

Group: nitrogen source
Media: DinoMM_noNitrogen_HighNutrient_GlucoseC + L-Glutamine (20 mM), pH=7
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.2 generations
By: Adam on 8-May-17
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 20 mM D-Glucose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1718 B3

Specific Phenotypes

For 6 genes in this experiment

For nitrogen source L-Glutamine in Echinicola vietnamensis KMM 6221, DSM 17526

For nitrogen source L-Glutamine across organisms

SEED Subsystems

Subsystem #Specific
NAD and NADP cofactor biosynthesis global 4
NAD regulation 1
Pentose phosphate pathway 1
Quinolinic acid and its derivatives 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde 5 4 3
NAD de novo biosynthesis II (from tryptophan) 9 8 5
NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde 4 4 2
3-hydroxy-4-methyl-anthranilate biosynthesis II 5 3 2
superpathway of NAD biosynthesis in eukaryotes 14 11 5
pentose phosphate pathway (oxidative branch) I 3 3 1
L-tryptophan degradation I (via anthranilate) 3 2 1
L-tryptophan degradation IX 12 4 3
L-tryptophan degradation III (eukaryotic) 15 7 3
L-tryptophan degradation XI (mammalian, via kynurenine) 23 8 4
NAD de novo biosynthesis III 6 4 1
NAD de novo biosynthesis IV (anaerobic) 6 4 1
NAD de novo biosynthesis I 6 4 1
3-hydroxy-4-methyl-anthranilate biosynthesis I 6 2 1
L-tryptophan degradation XII (Geobacillus) 12 3 2
2-nitrobenzoate degradation I 7 1 1
pentose phosphate pathway 8 8 1
nicotine biosynthesis 9 1 1
superpathway of nicotine biosynthesis 12 2 1
superpathway of glucose and xylose degradation 17 16 1
aspartate superpathway 25 20 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 5 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 4 1