Experiment set3IT056 for Echinicola vietnamensis KMM 6221, DSM 17526
L-Asparagine nitrogen source
Group: nitrogen sourceMedia: DinoMM_noNitrogen_HighNutrient_GlucoseC + L-Asparagine (20 mM), pH=7
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.9 generations
By: Adam on 8-May-17
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 20 mM D-Glucose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1718 B2
Specific Phenotypes
For 2 genes in this experiment
For nitrogen source L-Asparagine in Echinicola vietnamensis KMM 6221, DSM 17526
For nitrogen source L-Asparagine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Chorismate Synthesis | 1 |
| Phenylalanine and Tyrosine Branches from Chorismate | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-tyrosine biosynthesis I | 3 | 3 | 1 |
| L-tyrosine biosynthesis III | 4 | 2 | 1 |
| 4-hydroxy-3-prenylbenzoate biosynthesis | 4 | 1 | 1 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 1 |
| superpathway of novobiocin biosynthesis | 19 | 4 | 1 |
| superpathway of chorismate metabolism | 59 | 42 | 1 |