Experiment set3IT054 for Kangiella aquimarina DSM 16071

marine broth with Lomefloxacin hydrochloride 0.0032 mM

Group: stress
Media: marine_broth_2216 + Lomefloxacin hydrochloride (0.0032 mM)
Culturing: Kang_ML4, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 1/20/2015
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1095 D3,D4

Specific Phenotypes

For 10 genes in this experiment

For stress Lomefloxacin hydrochloride in Kangiella aquimarina DSM 16071

For stress Lomefloxacin hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	3
Multidrug Resistance Efflux Pumps	2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	2
Branched-Chain Amino Acid Biosynthesis	1
DNA-replication	1
Leucine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Valine, leucine and isoleucine biosynthesis
• Pyruvate metabolism
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-leucine biosynthesis	6	5	1
3-methylbutanol biosynthesis (engineered)	7	6	1
superpathway of branched chain amino acid biosynthesis	17	7	1