Experiment set3IT054 for Echinicola vietnamensis KMM 6221, DSM 17526

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L-Serine nitrogen source

Group: nitrogen source
Media: DinoMM_noNitrogen_HighNutrient_GlucoseC + L-Serine (20 mM), pH=7
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 3.7 generations
By: Adam on 8-May-17
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 20 mM D-Glucose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1718 A6

Specific Phenotypes

For 33 genes in this experiment

For nitrogen source L-Serine in Echinicola vietnamensis KMM 6221, DSM 17526

For nitrogen source L-Serine across organisms

SEED Subsystems

Subsystem #Specific
Ton and Tol transport systems 2
Aromatic amino acid degradation 1
Bacterial Cell Division 1
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. 1
Folate Biosynthesis 1
Glutathione-regulated potassium-efflux system and associated functions 1
NAD and NADP cofactor biosynthesis global 1
Potassium homeostasis 1
Queuosine-Archaeosine Biosynthesis 1
Soluble cytochromes and functionally related electron carriers 1
cAMP signaling in bacteria 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
4-aminobenzoate biosynthesis I 2 2 1
4-aminobenzoate biosynthesis II 2 1 1
L-tryptophan degradation I (via anthranilate) 3 2 1
L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde 5 4 1
3-hydroxy-4-methyl-anthranilate biosynthesis II 5 3 1
3-hydroxy-4-methyl-anthranilate biosynthesis I 6 2 1
NAD de novo biosynthesis II (from tryptophan) 9 8 1
superpathway of tetrahydrofolate biosynthesis 10 10 1
superpathway of candicidin biosynthesis 11 4 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 12 1
L-tryptophan degradation IX 12 4 1
L-tryptophan degradation XII (Geobacillus) 12 3 1
superpathway of NAD biosynthesis in eukaryotes 14 11 1
L-tryptophan degradation III (eukaryotic) 15 7 1
L-tryptophan degradation XI (mammalian, via kynurenine) 23 8 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 5 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 4 1
superpathway of chorismate metabolism 59 42 1