marine broth with D-Cycloserine 0.032 mg/ml
Group:
stress
Media:
marine_broth_2216 +
D-Cycloserine (0.032 mg/ml)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.0
generations
By: Adam on
8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L
Yeast Extract, 0.1 g/L
Ferric citrate, 19.45 g/L
Sodium Chloride, 5.9 g/L
Magnesium chloride hexahydrate, 3.24 g/L
Magnesium sulfate, 1.8 g/L
Calcium chloride, 0.55 g/L
Potassium Chloride, 0.16 g/L
Sodium bicarbonate, 0.08 g/L
Potassium bromide, 34 mg/L
Strontium chloride, 22 mg/L
Boric Acid, 4 mg/L
Sodium metasilicate, 2.4 mg/L
sodium fluoride, 8 mg/L
Disodium phosphate
Growth plate: 1740 C3
Specific Phenotypes
For 13 genes in this experiment
For stress D-Cycloserine in Pontibacter actiniarum KMM 6156, DSM 19842
For stress D-Cycloserine across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|
| UDP-α-D-glucuronate biosynthesis (from UDP-glucose) | 1 | 1 | 1 |
| L-threonine degradation III (to methylglyoxal) | 3 | 2 | 2 |
| L-threonine degradation II | 2 | 2 | 1 |
| UDP-α-D-xylose biosynthesis | 2 | 2 | 1 |
| aminopropanol phosphate biosynthesis II | 4 | 2 | 2 |
| UDP-N-acetyl-α-D-galactosaminuronate biosynthesis | 2 | 1 | 1 |
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| acrylate degradation II | 3 | 1 | 1 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 8 | 3 |
| adipate biosynthesis | 5 | 4 | 1 |
| adipate degradation | 5 | 4 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 1 |
| glutaryl-CoA degradation | 5 | 3 | 1 |
| acrylate degradation I | 5 | 1 | 1 |
| UDP-2,3-diacetamido-2,3-dideoxy-α-D-mannuronate biosynthesis | 5 | 1 | 1 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 1 | 1 |
| propanoyl-CoA degradation II | 5 | 1 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 1 | 1 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 2 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 5 | 2 |
| oleate β-oxidation | 35 | 23 | 6 |
| pyruvate fermentation to butanol II (engineered) | 6 | 5 | 1 |
| L-isoleucine degradation I | 6 | 4 | 1 |
| superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis | 6 | 4 | 1 |
| fatty acid salvage | 6 | 4 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 3 | 1 |
| propanoate fermentation to 2-methylbutanoate | 6 | 3 | 1 |
| β-alanine biosynthesis II | 6 | 1 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| pyruvate fermentation to butanoate | 7 | 3 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 3 | 1 |
| pyruvate fermentation to butanol I | 8 | 5 | 1 |
| L-valine degradation I | 8 | 3 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 6 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 11 | 2 |
| superpathway of L-threonine metabolism | 18 | 10 | 2 |
| valproate β-oxidation | 9 | 5 | 1 |
| UDP-sugars interconversion | 9 | 4 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 3 | 1 |
| teichuronic acid biosynthesis (B. subtilis 168) | 9 | 2 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 5 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 4 | 1 |
| 3-phenylpropanoate degradation | 10 | 3 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 1 |
| superpathway of phenylethylamine degradation | 11 | 6 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 3 | 2 |
| gallate degradation III (anaerobic) | 11 | 1 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 3 | 1 |
| 3-hydroxypropanoate cycle | 13 | 8 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 6 | 1 |
| glyoxylate assimilation | 13 | 5 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 3 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 3 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 10 | 1 |
| glycerol degradation to butanol | 16 | 11 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 3 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 6 | 1 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 3 | 1 |
| superpathway of the 3-hydroxypropanoate cycle | 18 | 8 | 1 |
| toluene degradation VI (anaerobic) | 18 | 3 | 1 |
| superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis | 24 | 6 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |