Experiment set3IT046 for Agrobacterium fabrum C58

L-Cysteine hydrochloride monohydrate nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Succinate_noNitrogen + L-Cysteine hydrochloride monohydrate (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 11/20/20
Media components: 10 mM Sodium succinate dibasic hexahydrate, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 10 genes in this experiment

For nitrogen source L-Cysteine hydrochloride monohydrate in Agrobacterium fabrum C58

For nitrogen source L-Cysteine hydrochloride monohydrate across organisms

SEED Subsystems

Subsystem	#Specific
Terminal cytochrome d ubiquinol oxidases	4
Terminal cytochrome oxidases	4
Bacterial RNA-metabolizing Zn-dependent hydrolases	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Alanine and aspartate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NADH to cytochrome bd oxidase electron transfer I	2	2	1
succinate to cytochrome bd oxidase electron transfer	2	2	1
NADH to cytochrome bd oxidase electron transfer II	2	1	1
pyruvate to cytochrome bd oxidase electron transfer	2	1	1