Experiment set3IT044 for Echinicola vietnamensis KMM 6221, DSM 17526

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L-tyrosine disodium salt nitrogen source

Group: nitrogen source
Media: DinoMM_noNitrogen_HighNutrient_GlucoseC + L-tyrosine disodium salt (20 mM), pH=7
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.8 generations
By: Adam on 8-May-17
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 20 mM D-Glucose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1717 D1

Specific Phenotypes

For 82 genes in this experiment

For nitrogen source L-tyrosine disodium salt in Echinicola vietnamensis KMM 6221, DSM 17526

For nitrogen source L-tyrosine disodium salt across organisms

SEED Subsystems

Subsystem #Specific
Thiamin biosynthesis 3
Cobalt-zinc-cadmium resistance 2
D-Galacturonate and D-Glucuronate Utilization 2
Multidrug Resistance Efflux Pumps 2
Ammonia assimilation 1
Arginine Deiminase Pathway 1
Arginine and Ornithine Degradation 1
Bacterial Cell Division 1
Chitin and N-acetylglucosamine utilization 1
Copper homeostasis 1
DNA repair, bacterial MutL-MutS system 1
Experimental tye 1
Glutathione-regulated potassium-efflux system and associated functions 1
KDO2-Lipid A biosynthesis 1
Lipid A-Ara4N pathway ( Polymyxin resistance ) 1
Menaquinone and Phylloquinone Biosynthesis 1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 1
N-linked Glycosylation in Bacteria 1
NAD and NADP cofactor biosynthesis global 1
Potassium homeostasis 1
Purine conversions 1
Rhamnose containing glycans 1
Teichoic and lipoteichoic acids biosynthesis 1
Teichuronic acid biosynthesis 1
Transport of Manganese 1
Two cell division clusters relating to chromosome partitioning 1
Universal GTPases 1
Xylose utilization 1
dTDP-rhamnose synthesis 1
linker unit-arabinogalactan synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-glucuronate biosynthesis (from UDP-glucose) 1 1 1
UDP-α-D-xylose biosynthesis 2 2 1
xanthine and xanthosine salvage 2 2 1
thiamine diphosphate biosynthesis I (E. coli) 2 2 1
(1,4)-β-D-xylan degradation 2 2 1
guanine and guanosine salvage I 2 2 1
thiamine diphosphate biosynthesis II (Bacillus) 2 2 1
trehalose degradation I (low osmolarity) 2 1 1
trehalose degradation II (cytosolic) 2 1 1
guanine and guanosine salvage II 2 1 1
thiamine diphosphate salvage V 3 3 1
thiazole component of thiamine diphosphate biosynthesis I 6 5 2
trehalose degradation IV 3 2 1
trehalose degradation V 3 2 1
thiamine diphosphate biosynthesis III (Staphylococcus) 3 2 1
superpathway of guanine and guanosine salvage 3 2 1
adenine salvage 3 2 1
GDP-α-D-glucose biosynthesis 3 2 1
thiamine diphosphate biosynthesis IV (eukaryotes) 3 2 1
cellulose and hemicellulose degradation (cellulolosome) 3 1 1
superpathway of thiamine diphosphate biosynthesis I 10 9 3
D-fructuronate degradation 4 4 1
L-arginine degradation V (arginine deiminase pathway) 4 4 1
sucrose degradation III (sucrose invertase) 4 3 1
adenine and adenosine salvage III 4 3 1
dTDP-6-deoxy-α-D-allose biosynthesis 4 2 1
dTDP-N-acetylviosamine biosynthesis 4 2 1
dTDP-β-D-fucofuranose biosynthesis 4 2 1
dTDP-N-acetylthomosamine biosynthesis 4 2 1
phenol degradation II (anaerobic) 4 1 1
CMP-3-deoxy-D-manno-octulosonate biosynthesis 5 5 1
L-arginine degradation XIII (reductive Stickland reaction) 5 5 1
dTDP-β-L-rhamnose biosynthesis 5 5 1
thiamine diphosphate salvage II 5 5 1
L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde 5 4 1
glucose and glucose-1-phosphate degradation 5 3 1
dTDP-4-O-demethyl-β-L-noviose biosynthesis 5 3 1
3-hydroxy-4-methyl-anthranilate biosynthesis II 5 3 1
dTDP-α-D-mycaminose biosynthesis 5 2 1
Salmonella enterica serotype O:54 O antigen biosynthesis 5 2 1
dTDP-3-acetamido-α-D-fucose biosynthesis 5 2 1
dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis 5 2 1
superpathway of thiamine diphosphate biosynthesis II 11 9 2
UDP-N-acetyl-D-glucosamine biosynthesis II 6 3 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 3 1
dTDP-L-daunosamine biosynthesis 6 3 1
glycogen degradation II 6 3 1
dTDP-sibirosamine biosynthesis 6 3 1
dTDP-D-desosamine biosynthesis 6 2 1
3-hydroxy-4-methyl-anthranilate biosynthesis I 6 2 1
dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis 6 2 1
L-arginine degradation XIV (oxidative Stickland reaction) 6 2 1
superpathway of β-D-glucuronosides degradation 7 6 1
2-carboxy-1,4-naphthoquinol biosynthesis 7 6 1
thiamine diphosphate salvage IV (yeast) 7 6 1
thiazole component of thiamine diphosphate biosynthesis II 7 5 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 4 1
CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis 7 4 1
superpathway of thiamine diphosphate biosynthesis III (eukaryotes) 7 4 1
dTDP-β-L-digitoxose biosynthesis 7 3 1
dTDP-β-L-olivose biosynthesis 7 3 1
dTDP-β-L-mycarose biosynthesis 7 2 1
thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) 8 5 1
dTDP-β-L-megosamine biosynthesis 8 3 1
glycogen degradation I 8 3 1
sucrose biosynthesis II 8 3 1
dTDP-β-L-4-epi-vancosamine biosynthesis 8 3 1
NAD de novo biosynthesis II (from tryptophan) 9 8 1
superpathway of demethylmenaquinol-8 biosynthesis I 9 8 1
superpathway of demethylmenaquinol-9 biosynthesis 9 7 1
superpathway of demethylmenaquinol-6 biosynthesis I 9 7 1
1,3-propanediol biosynthesis (engineered) 9 6 1
chitin biosynthesis 9 4 1
dTDP-α-D-forosamine biosynthesis 9 3 1
teichuronic acid biosynthesis (B. subtilis 168) 9 3 1
dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis 9 3 1
UDP-sugars interconversion 9 3 1
superpathway of menaquinol-8 biosynthesis I 10 9 1
superpathway of hexuronide and hexuronate degradation 10 9 1
superpathway of menaquinol-10 biosynthesis 10 8 1
superpathway of menaquinol-12 biosynthesis 10 8 1
superpathway of menaquinol-13 biosynthesis 10 8 1
superpathway of menaquinol-6 biosynthesis 10 8 1
superpathway of menaquinol-7 biosynthesis 10 8 1
superpathway of menaquinol-9 biosynthesis 10 8 1
superpathway of menaquinol-11 biosynthesis 10 8 1
superpathway of enterobacterial common antigen biosynthesis 10 3 1
poly(3-O-β-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis 10 2 1
glycolysis III (from glucose) 11 10 1
colanic acid building blocks biosynthesis 11 9 1
O-antigen building blocks biosynthesis (E. coli) 11 8 1
poly(glycerol phosphate) wall teichoic acid biosynthesis 11 3 1
homolactic fermentation 12 10 1
L-tryptophan degradation IX 12 4 1
poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis) 12 2 1
superpathway of purine nucleotide salvage 14 12 1
superpathway of NAD biosynthesis in eukaryotes 14 11 1
poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus) 14 2 1
Bifidobacterium shunt 15 11 1
L-tryptophan degradation III (eukaryotic) 15 7 1
superpathway of phylloquinol biosynthesis 15 7 1
superpathway of (Kdo)2-lipid A biosynthesis 17 13 1
heterolactic fermentation 18 12 1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis 19 5 1
superpathway of novobiocin biosynthesis 19 4 1
superpathway of erythromycin biosynthesis 19 2 1
superpathway of megalomicin A biosynthesis 22 3 1
L-tryptophan degradation XI (mammalian, via kynurenine) 23 8 1
superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis 23 3 1
superpathway of Kdo2-lipid A biosynthesis 25 13 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 2 1
superpathway of microbial D-galacturonate and D-glucuronate degradation 31 14 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 10 1
superpathway of chorismate metabolism 59 42 1