Experiment set3IT039 for Pseudomonas simiae WCS417

LB with Nalidixic acid sodium salt 0.025 mg/ml

Group: stress
Media: LB + Nalidixic acid sodium salt (0.025 mg/ml)
Culturing: fluoroDangl_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1165 E1,E2

Specific Phenotypes

For 11 genes in this experiment

For stress Nalidixic acid sodium salt in Pseudomonas simiae WCS417

For stress Nalidixic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	4
DNA-replication	1
DNA repair, bacterial DinG and relatives	1
Multidrug Resistance Efflux Pumps	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
N-linked Glycosylation in Bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Tyrosine metabolism
• Benzoate degradation via hydroxylation
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Nucleotide sugars metabolism
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-N-acetyl-β-L-fucosamine biosynthesis	4	2	1
UDP-N-acetyl-β-L-quinovosamine biosynthesis	4	1	1
CMP-pseudaminate biosynthesis	6	1	1
CMP-diacetamido-8-epilegionaminic acid biosynthesis	7	1	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	9	1