Experiment set3IT038 for Echinicola vietnamensis KMM 6221, DSM 17526

L-Glutamine nitrogen source

Group: nitrogen source
Media: DinoMM_noNitrogen_HighNutrient_GlucoseC + L-Glutamine (20 mM), pH=7
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.2 generations
By: Adam on 8-May-17
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 20 mM D-Glucose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1717 B3

Specific Phenotypes

For 7 genes in this experiment

For nitrogen source L-Glutamine in Echinicola vietnamensis KMM 6221, DSM 17526

For nitrogen source L-Glutamine across organisms

SEED Subsystems

Subsystem	#Specific
NAD and NADP cofactor biosynthesis global	4
NAD regulation	1
Pentose phosphate pathway	1
Quinolinic acid and its derivatives	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tryptophan metabolism
• Pentose phosphate pathway
• Glutathione metabolism
• Nicotinate and nicotinamide metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde	5	4	3
NAD de novo biosynthesis II (from tryptophan)	9	8	5
NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde	4	4	2
3-hydroxy-4-methyl-anthranilate biosynthesis II	5	3	2
superpathway of NAD biosynthesis in eukaryotes	14	11	5
pentose phosphate pathway (oxidative branch) I	3	3	1
L-tryptophan degradation I (via anthranilate)	3	2	1
L-tryptophan degradation IX	12	4	3
L-tryptophan degradation III (eukaryotic)	15	7	3
L-tryptophan degradation XI (mammalian, via kynurenine)	23	8	4
NAD de novo biosynthesis I	6	4	1
NAD de novo biosynthesis IV (anaerobic)	6	4	1
NAD de novo biosynthesis III	6	4	1
3-hydroxy-4-methyl-anthranilate biosynthesis I	6	2	1
L-tryptophan degradation XII (Geobacillus)	12	3	2
2-nitrobenzoate degradation I	7	1	1
pentose phosphate pathway	8	8	1
nicotine biosynthesis	9	1	1
superpathway of nicotine biosynthesis	12	2	1
superpathway of glucose and xylose degradation	17	16	1
aspartate superpathway	25	20	1
superpathway of aromatic compound degradation via 3-oxoadipate	35	5	1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate	42	4	1