Experiment set3IT034 for Escherichia coli BW25113

Compare to:

deuterated glucose in D2O

200 most important genes:

  gene name fitness t score description  
b0907 serC -7.0 -9.6 phosphoserine aminotransferase (NCBI) compare
b2020 hisD -6.0 -9.9 histidinol dehydrogenase (NCBI) compare
b1192 ldcA -5.8 -5.6 L,D-carboxypeptidase A (NCBI) compare
b2913 serA -5.7 -7.9 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b0423 thiI -5.5 -8.4 thiamine biosynthesis protein ThiI (NCBI) compare
b1260 trpA -5.4 -7.9 tryptophan synthase subunit alpha (NCBI) compare
b0523 purE -5.3 -7.3 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b3771 ilvD -5.3 -16.3 dihydroxy-acid dehydratase (NCBI) compare
b3941 metF -5.3 -14.5 5,10-methylenetetrahydrofolate reductase (NCBI) compare
b2025 hisF -5.3 -6.3 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b4388 serB -5.3 -9.5 3-phosphoserine phosphatase (NCBI) compare
b0003 thrB -5.1 -12.2 homoserine kinase (NCBI) compare
b0004 thrC -5.0 -9.7 threonine synthase (NCBI) compare
b0145 dksA -4.9 -3.4 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3829 metE -4.9 -20.2 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI) compare
b2022 hisB -4.9 -10.0 imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS) compare
b3774 ilvC -4.8 -20.0 ketol-acid reductoisomerase (NCBI) compare
b1062 pyrC -4.8 -11.6 dihydroorotase (NCBI) compare
b2329 aroC -4.8 -8.0 chorismate synthase (NCBI) compare
b4006 purH -4.7 -16.4 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b2557 purL -4.6 -12.7 phosphoribosylformylglycinamidine synthase (NCBI) compare
b4005 purD -4.6 -12.6 phosphoribosylamine--glycine ligase (NCBI) compare
b0908 aroA -4.6 -12.2 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3389 aroB -4.5 -17.7 3-dehydroquinate synthase (NCBI) compare
b2024 hisA -4.5 -2.8 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b2026 hisI -4.5 -11.9 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI) compare
b2019 hisG -4.5 -6.3 ATP phosphoribosyltransferase (NCBI) compare
b2312 purF -4.4 -11.3 amidophosphoribosyltransferase (NCBI) compare
b3414 gntY -4.4 -9.3 predicted gluconate transport associated protein (NCBI) compare
b2499 purM -4.3 -7.8 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2762 cysH -4.3 -11.0 phosphoadenosine phosphosulfate reductase (NCBI) compare
b0592 fepB -4.3 -13.0 iron-enterobactin transporter subunit (NCBI) compare
b1281 pyrF -4.3 -3.0 orotidine 5'-phosphate decarboxylase (NCBI) compare
b3828 metR -4.3 -13.9 DNA-binding transcriptional activator, homocysteine-binding (NCBI) compare
b0585 fes -4.2 -9.4 enterobactin/ferric enterobactin esterase (NCBI) compare
b3770 ilvE -4.2 -13.3 branched-chain amino acid aminotransferase (NCBI) compare
b3281 aroE -4.2 -21.0 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b0049 apaH -4.2 -8.4 diadenosinetetraphosphatase (NCBI) compare
b0242 proB -4.2 -9.2 gamma-glutamyl kinase (NCBI) compare
b0386 proC -4.1 -9.5 pyrroline-5-carboxylate reductase (NCBI) compare
b2023 hisH -4.1 -7.5 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b0243 proA -4.1 -13.0 gamma-glutamyl phosphate reductase (NCBI) compare
b2476 purC -4.1 -4.3 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1693 aroD -4.1 -9.3 3-dehydroquinate dehydratase (NCBI) compare
b2021 hisC -3.9 -6.0 histidinol-phosphate aminotransferase (NCBI) compare
b3960 argH -3.8 -15.5 argininosuccinate lyase (NCBI) compare
b3172 argG -3.8 -15.0 argininosuccinate synthase (NCBI) compare
b0588 fepC -3.8 -10.9 iron-enterobactin transporter subunit (NCBI) compare
b3619 rfaD -3.8 -7.2 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b4214 cysQ -3.7 -15.9 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b0078 ilvH -3.7 -11.2 acetolactate synthase small subunit (NCBI) compare
b0439 lon -3.6 -7.7 DNA-binding ATP-dependent protease La (NCBI) compare
b3991 thiG -3.6 -10.0 thiamin biosynthesis, thiazole moiety (VIMSS) compare
b0928 aspC -3.6 -9.7 aspartate aminotransferase, PLP-dependent (NCBI) compare
b4407 thiS -3.6 -2.8 sulfur carrier protein ThiS (NCBI) compare
b0945 pyrD -3.6 -13.7 dihydroorotate dehydrogenase (NCBI) compare
b0077 ilvI -3.5 -15.6 acetolactate synthase III large subunit (NCBI) compare
b0002 thrA -3.5 -16.6 bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI) compare
b0033 carB -3.5 -17.6 carbamoyl-phosphate synthase large subunit (NCBI) compare
b0522 purK -3.5 -8.7 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b2320 pdxB -3.4 -11.8 erythronate-4-phosphate dehydrogenase (NCBI) compare
b0683 fur -3.3 -2.8 ferric uptake regulator (NCBI) compare
b3992 thiF -3.3 -12.6 thiamin biosynthesis, thiazole moiety (VIMSS) compare
b3993 thiE -3.2 -10.7 thiamine-phosphate pyrophosphorylase (NCBI) compare
b0437 clpP -3.2 -10.2 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0052 pdxA -3.2 -13.4 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI) compare
b3741 gidA -3.2 -11.1 glucose-inhibited division protein A (NCBI) compare
b3706 trmE -3.2 -10.2 tRNA modification GTPase (NCBI) compare
b3994 thiC -3.2 -16.5 thiamine biosynthesis protein ThiC (NCBI) compare
b1261 trpB -3.1 -3.1 tryptophan synthase subunit beta (NCBI) compare
b3169 nusA -3.1 -5.9 transcription elongation factor NusA (NCBI) compare
b0589 fepG -3.1 -10.4 iron-enterobactin transporter subunit (NCBI) compare
b4245 pyrB -3.1 -13.4 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b0590 fepD -3.0 -9.2 iron-enterobactin transporter subunit (NCBI) compare
b1712 ihfA -3.0 -3.9 integration host factor subunit alpha (NCBI) compare
b2188 yejM -3.0 -13.4 predicted hydrolase, inner membrane (NCBI) compare
b2414 cysK -3.0 -12.0 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b3204 ptsN -3.0 -7.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2750 cysC -3.0 -10.9 adenylylsulfate kinase (NCBI) compare
b3870 glnA -3.0 -2.9 glutamine synthetase (NCBI) compare
b3390 aroK -2.9 -3.8 shikimate kinase I (NCBI) compare
b3916 pfkA -2.9 -4.8 6-phosphofructokinase (NCBI) compare
b3990 thiH -2.9 -12.3 thiamine biosynthesis protein ThiH (NCBI) compare
b4177 purA -2.9 -2.8 adenylosuccinate synthetase (NCBI) compare
b3772 ilvA -2.7 -15.2 threonine dehydratase (NCBI) compare
b2564 pdxJ -2.7 -6.1 pyridoxal phosphate biosynthetic protein (NCBI) compare
b3956 ppc -2.7 -18.2 phosphoenolpyruvate carboxylase (NCBI) compare
b3290 trkA -2.7 -5.4 potassium transporter peripheral membrane component (NCBI) compare
b3650 spoT -2.7 -8.9 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b2618 b2618 -2.6 -5.1 orf, hypothetical protein (VIMSS) compare
b2103 thiD -2.6 -8.7 phosphomethylpyrimidine kinase (NCBI) compare
b2838 lysA -2.6 -12.4 diaminopimelate decarboxylase, PLP-binding (NCBI) compare
b3008 metC -2.6 -13.1 cystathionine beta-lyase (NCBI) compare
b3483 yhhH -2.6 -3.4 orf, hypothetical protein (VIMSS) compare
b0074 leuA -2.6 -11.4 2-isopropylmalate synthase (NCBI) compare
b2820 recB -2.6 -3.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3642 pyrE -2.5 -5.0 orotate phosphoribosyltransferase (NCBI) compare
b1262 trpC -2.5 -4.4 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b1865 nudB -2.5 -3.2 dATP pyrophosphohydrolase (NCBI) compare
b3863 polA -2.4 -1.6 DNA polymerase I (NCBI) compare
b1685 ydiH -2.4 -0.9 predicted protein (RefSeq) compare
b2600 tyrA -2.4 -8.5 fused chorismate mutase T/prephenate dehydrogenase (NCBI) compare
b1084 rne -2.4 -7.5 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b0118 acnB -2.3 -5.5 aconitate hydratase (NCBI) compare
b0750 nadA -2.3 -8.0 quinolinate synthetase (NCBI) compare
b1627 rsxA -2.3 -6.2 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b3621 rfaC -2.3 -8.3 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2424 cysU -2.2 -4.6 sulfate/thiosulfate transporter subunit (NCBI) compare
b3052 rfaE -2.2 -8.4 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1263 trpD -2.2 -3.0 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b0032 carA -2.2 -7.7 carbamoyl-phosphate synthase small subunit (NCBI) compare
b4054 tyrB -2.2 -10.9 tyrosine aminotransferase, tyrosine-repressible, PLP-dependent (NCBI) compare
b2764 cysJ -2.1 -10.0 sulfite reductase, alpha subunit, flavoprotein (NCBI) compare
b0912 ihfB -2.1 -4.8 integration host factor subunit beta (NCBI) compare
b0222 lpcA -2.1 -3.7 phosphoheptose isomerase (NCBI) compare
b0890 ftsK -2.1 -9.8 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b3026 qseC -2.1 -8.2 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2818 argA -2.1 -7.8 N-acetylglutamate synthase (NCBI) compare
b3387 dam -2.1 -13.5 DNA adenine methylase (NCBI) compare
b2763 cysI -2.1 -9.7 sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI) compare
b3386 rpe -2.1 -2.0 ribulose-phosphate 3-epimerase (NCBI) compare
b1252 tonB -2.0 -5.2 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2501 ppk -2.0 -10.7 polyphosphate kinase (NCBI) compare
b4171 miaA -2.0 -8.4 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b3620 rfaF -2.0 -6.4 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2894 xerD -2.0 -2.6 site-specific tyrosine recombinase XerD (NCBI) compare
b2830 nudH -2.0 -3.2 dinucleoside polyphosphate hydrolase (NCBI) compare
b3958 argC -1.9 -11.1 N-acetyl-gamma-glutamyl-phosphate reductase (NCBI) compare
b3957 argE -1.9 -12.7 acetylornithine deacetylase (NCBI) compare
b0113 pdhR -1.9 -2.4 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b2528 iscA -1.9 -2.5 iron-sulfur cluster assembly protein (NCBI) compare
b2500 purN -1.9 -4.9 phosphoribosylglycinamide formyltransferase (NCBI) compare
b0142 folK -1.9 -1.0 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1628 rsxB -1.8 -5.5 electron transport complex protein RnfB (NCBI) compare
b3649 rpoZ -1.8 -3.5 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3368 cysG -1.8 -12.0 fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI) compare
b0889 lrp -1.8 -3.6 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1863 ruvC -1.7 -4.0 Holliday junction resolvase (NCBI) compare
b2570 rseC -1.7 -7.8 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b0200 gmhB -1.7 -2.9 hypothetical protein (NCBI) compare
b1273 yciN -1.7 -2.3 hypothetical protein (NCBI) compare
b3849 trkH -1.7 -11.3 potassium transporter (NCBI) compare
b3811 xerC -1.6 -4.1 site-specific tyrosine recombinase XerC (NCBI) compare
b1545 pinQ -1.6 -2.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b2574 nadB -1.6 -9.5 L-aspartate oxidase (NCBI) compare
b3006 exbB -1.6 -7.9 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1860 ruvB -1.6 -2.5 Holliday junction DNA helicase B (NCBI) compare
b1684 sufA -1.6 -2.8 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b3959 argB -1.6 -8.4 acetylglutamate kinase (RefSeq) compare
b1290 sapF -1.6 -2.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1632 rsxE -1.5 -7.5 NADH-ubiquinone oxidoreductase (NCBI) compare
b3773 ilvY -1.5 -9.2 DNA-binding transcriptional dual regulator (NCBI) compare
b0738 tolR -1.5 -4.9 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1871 yecP -1.5 -5.2 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b1630 rsxD -1.5 -6.4 electron transport complex protein RnfD (NCBI) compare
b0073 leuB -1.5 -9.6 3-isopropylmalate dehydrogenase (VIMSS) compare
b3764 yifE -1.5 -4.3 hypothetical protein (NCBI) compare
b2599 pheA -1.5 -5.3 fused chorismate mutase P/prephenate dehydratase (NCBI) compare
b1629 rsxC -1.5 -6.3 electron transport complex protein RnfC (NCBI) compare
b2839 lysR -1.5 -6.8 DNA-binding transcriptional dual regulator (NCBI) compare
b2822 recC -1.4 -6.5 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b4435 isrC -1.4 -2.9 unknown RNA (NCBI) compare
b1897 otsB -1.4 -2.9 trehalose-6-phosphate phosphatase, biosynthetic (NCBI) compare
b0739 tolA -1.4 -5.2 cell envelope integrity inner membrane protein TolA (NCBI) compare
b1778 yeaA -1.4 -3.4 methionine sulfoxide reductase B (NCBI) compare
b0957 ompA -1.4 -2.7 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b0109 nadC -1.4 -5.7 nicotinate-nucleotide pyrophosphorylase (NCBI) compare
b2029 gnd -1.4 -7.4 6-phosphogluconate dehydrogenase (NCBI) compare
b0603 ybdO -1.4 -3.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b0053 surA -1.4 -6.4 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0075 leuL -1.4 -4.3 leu operon leader peptide (NCBI) compare
b3005 exbD -1.3 -4.3 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b3121 yhaC -1.3 -2.2 hypothetical protein (NCBI) compare
b2055 wcaE -1.3 -2.1 predicted glycosyl transferase (NCBI) compare
b2214 yojL -1.3 -6.7 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b3769 ilvM -1.3 -3.3 acetolactate synthase II, small subunit (NCBI) compare
b1235 rssB -1.3 -7.0 response regulator of RpoS (NCBI) compare
b3778 rep -1.3 -8.5 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b4704 arrS -1.3 -1.8 Antisense sRNA ArrS, function unknown (NCBI) compare
b1291 sapD -1.3 -2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4243 yjgF -1.3 -5.0 orf, hypothetical protein (VIMSS) compare
b1603 pntA -1.2 -4.2 NAD(P) transhydrogenase subunit alpha (NCBI) compare
b4025 pgi -1.2 -7.8 glucose-6-phosphate isomerase (NCBI) compare
b2853 ygeI -1.2 -2.3 hypothetical protein (NCBI) compare
b0688 pgm -1.2 -4.5 phosphoglucomutase (NCBI) compare
b1264 trpE -1.2 -4.0 anthranilate synthase component I (NCBI) compare
b2683 ygaH -1.2 -6.9 predicted inner membrane protein (NCBI) compare
b0028 fkpB -1.2 -4.1 FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI) compare
b3844 fre -1.2 -5.6 NAD(P)H-flavin reductase (NCBI) compare
b3652 recG -1.2 -8.1 ATP-dependent DNA helicase (NCBI) compare
b4697 yrdF -1.2 -3.5 no description compare
b4480 hdfR -1.2 -5.3 transcriptional regulator HdfR (NCBI) compare
b3780 rhlB -1.2 -7.4 ATP-dependent RNA helicase (NCBI) compare
b1730 ydjO -1.1 -2.1 predicted protein (RefSeq) compare
b3725 pstB -1.1 -3.3 phosphate transporter subunit (NCBI) compare
b1144 ymfJ -1.1 -2.3 orf, hypothetical protein (VIMSS) compare
b2752 cysD -1.1 -4.6 sulfate adenylyltransferase subunit 2 (NCBI) compare
b0740 tolB -1.1 -5.2 translocation protein TolB precursor (NCBI) compare
b0122 yacC -1.1 -2.6 orf, hypothetical protein (VIMSS) compare
b3630 rfaP -1.1 -8.3 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare


Specific Phenotypes

For 15 genes in this experiment