Experiment set3IT034 for Agrobacterium fabrum C58

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N-Acetyl-D-Glucosamine nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Succinate_noNitrogen + N-Acetyl-D-Glucosamine (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 11/20/20
Media components: 10 mM Sodium succinate dibasic hexahydrate, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 12 genes in this experiment

For nitrogen source N-Acetyl-D-Glucosamine in Agrobacterium fabrum C58

For nitrogen source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 3
Sialic Acid Metabolism 2
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 2
Inositol catabolism 1
Maltose and Maltodextrin Utilization 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 2
N-acetylglucosamine degradation II 3 2 2
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 3 3
D-galactosamine and N-acetyl-D-galactosamine degradation 4 3 1
chitin derivatives degradation 8 2 2
N-acetylneuraminate and N-acetylmannosamine degradation I 4 1 1
UDP-N-acetyl-D-glucosamine biosynthesis I 5 5 1
N-acetyl-D-galactosamine degradation 5 3 1
CMP-N-acetylneuraminate biosynthesis I (eukaryotes) 5 1 1
UDP-N-acetyl-D-glucosamine biosynthesis II 6 4 1
UDP-N-acetyl-D-galactosamine biosynthesis III 6 2 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
chitin degradation I (archaea) 7 1 1
superpathway of N-acetylneuraminate degradation 22 12 3
photorespiration III 9 6 1
photorespiration I 9 6 1
chitin biosynthesis 9 5 1
photorespiration II 10 6 1
CMP-legionaminate biosynthesis I 10 2 1
O-antigen building blocks biosynthesis (E. coli) 11 11 1
peptidoglycan recycling I 14 8 1
superpathway of CMP-sialic acids biosynthesis 15 1 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 6 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 13 1