Experiment set3IT033 for Pontibacter actiniarum KMM 6156, DSM 19842

Compare to:

marine broth with benzoic acid 0.4 mg/ml

Group: stress
Media: marine_broth_2216 + benzoic acid (0.4 mg/ml)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.9 generations
By: Adam on 8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1738 B5

Specific Phenotypes

For 1 genes in this experiment

For stress benzoic acid in Pontibacter actiniarum KMM 6156, DSM 19842

For stress benzoic acid across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 1
Glycine and Serine Utilization 1
Methionine Biosynthesis 1
Methionine Degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-cysteine degradation II 3 3 3
D-serine degradation 3 3 2
L-serine degradation 3 3 2
L-methionine degradation II 3 3 2
L-tryptophan degradation II (via pyruvate) 3 2 2
L-cysteine biosynthesis III (from L-homocysteine) 2 2 1
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
L-methionine biosynthesis II 6 3 2
L-threonine degradation I 6 2 2
L-isoleucine biosynthesis I (from threonine) 7 6 2
glycine betaine degradation III 7 4 2
homocysteine and cysteine interconversion 4 3 1
glycine betaine degradation I 8 4 2
L-mimosine degradation 8 4 2
glutathione-mediated detoxification I 8 2 2
superpathway of L-cysteine biosynthesis (mammalian) 5 4 1
superpathway of L-cysteine biosynthesis (fungi) 6 5 1
hydrogen sulfide biosynthesis II (mammalian) 6 4 1
superpathway of L-isoleucine biosynthesis I 13 11 2
L-cysteine biosynthesis VI (reverse transsulfuration) 7 4 1
hypoglycin biosynthesis 14 3 2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 11 2
superpathway of branched chain amino acid biosynthesis 17 16 2
superpathway of L-threonine metabolism 18 10 2
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 9 1
purine nucleobases degradation II (anaerobic) 24 13 2
superpathway of L-methionine salvage and degradation 16 9 1