Experiment set3IT033 for Echinicola vietnamensis KMM 6221, DSM 17526
L-Lysine nitrogen source
Group: nitrogen sourceMedia: DinoMM_noNitrogen_HighNutrient_GlucoseC + L-Lysine (20 mM), pH=7
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.4 generations
By: Adam on 8-May-17
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 20 mM D-Glucose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1717 A3
Specific Phenotypes
For 58 genes in this experiment
For nitrogen source L-Lysine in Echinicola vietnamensis KMM 6221, DSM 17526
For nitrogen source L-Lysine across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nucleotide sugars metabolism
- Starch and sucrose metabolism
- Polyketide sugar unit biosynthesis
- Glycolysis / Gluconeogenesis
- Ascorbate and aldarate metabolism
- Lysine biosynthesis
- Lysine degradation
- Streptomycin biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
- Pentose and glucuronate interconversions
- Fructose and mannose metabolism
- Galactose metabolism
- Ubiquinone and menaquinone biosynthesis
- C21-Steroid hormone metabolism
- Urea cycle and metabolism of amino groups
- Purine metabolism
- Puromycin biosynthesis
- Glycine, serine and threonine metabolism
- Arginine and proline metabolism
- Bisphenol A degradation
- Glutathione metabolism
- Aminosugars metabolism
- Lipopolysaccharide biosynthesis
- Linoleic acid metabolism
- Tetrachloroethene degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Retinol metabolism
- Porphyrin and chlorophyll metabolism
- Nitrogen metabolism
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of vancomycin group antibiotics
- Biosynthesis of type II polyketide products
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- T cell receptor signaling pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: