Experiment set3IT033 for Agrobacterium fabrum C58
Hydantoin nitrogen source
Group: nitrogen sourceMedia: MOPS minimal media_Succinate_noNitrogen + Hydantoin (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 11/20/20
Media components: 10 mM Sodium succinate dibasic hexahydrate, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 6 genes in this experiment
For nitrogen source Hydantoin in Agrobacterium fabrum C58
For nitrogen source Hydantoin across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fructose and mannose metabolism
- Pyrimidine metabolism
- beta-Alanine metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Pantothenate and CoA biosynthesis
- Drug metabolism - other enzymes
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-sorbitol degradation I | 3 | 3 | 1 |
| uracil degradation I (reductive) | 3 | 2 | 1 |
| thymine degradation | 3 | 2 | 1 |
| sucrose degradation I (sucrose phosphotransferase) | 3 | 1 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 4 | 1 |
| sucrose degradation IV (sucrose phosphorylase) | 4 | 4 | 1 |
| sucrose degradation VII (sucrose 3-dehydrogenase) | 4 | 2 | 1 |
| mannitol cycle | 5 | 4 | 1 |
| sucrose degradation II (sucrose synthase) | 5 | 4 | 1 |
| superpathway of pyrimidine ribonucleosides degradation | 5 | 4 | 1 |
| heterolactic fermentation | 18 | 12 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 13 | 1 |