Experiment set3IT032 for Escherichia coli BW25113

Compare to:

deuterated glucose in H2O

200 most important genes:

  gene name fitness t score description  
b2020 hisD -6.1 -7.3 histidinol dehydrogenase (NCBI) compare
b1260 trpA -5.9 -4.8 tryptophan synthase subunit alpha (NCBI) compare
b0907 serC -5.9 -8.0 phosphoserine aminotransferase (NCBI) compare
b2022 hisB -5.8 -6.9 imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS) compare
b2023 hisH -5.8 -5.6 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b3389 aroB -5.8 -11.1 3-dehydroquinate synthase (NCBI) compare
b2838 lysA -5.8 -5.6 diaminopimelate decarboxylase, PLP-binding (NCBI) compare
b0071 leuD -5.7 -5.6 isopropylmalate isomerase small subunit (NCBI) compare
b0073 leuB -5.7 -10.2 3-isopropylmalate dehydrogenase (VIMSS) compare
b3281 aroE -5.7 -14.7 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3774 ilvC -5.7 -11.5 ketol-acid reductoisomerase (NCBI) compare
b0072 leuC -5.6 -10.1 isopropylmalate isomerase large subunit (NCBI) compare
b0523 purE -5.6 -5.4 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b0243 proA -5.6 -6.6 gamma-glutamyl phosphate reductase (NCBI) compare
b0074 leuA -5.5 -10.0 2-isopropylmalate synthase (NCBI) compare
b2312 purF -5.5 -6.5 amidophosphoribosyltransferase (NCBI) compare
b1261 trpB -5.4 -3.0 tryptophan synthase subunit beta (NCBI) compare
b3941 metF -5.4 -11.4 5,10-methylenetetrahydrofolate reductase (NCBI) compare
b2025 hisF -5.3 -3.7 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b2019 hisG -5.2 -6.2 ATP phosphoribosyltransferase (NCBI) compare
b0033 carB -5.2 -15.9 carbamoyl-phosphate synthase large subunit (NCBI) compare
b4006 purH -5.1 -12.0 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b4005 purD -5.1 -8.5 phosphoribosylamine--glycine ligase (NCBI) compare
b0945 pyrD -5.0 -9.0 dihydroorotate dehydrogenase (NCBI) compare
b2329 aroC -5.0 -3.4 chorismate synthase (NCBI) compare
b3828 metR -4.9 -9.5 DNA-binding transcriptional activator, homocysteine-binding (NCBI) compare
b1693 aroD -4.9 -8.9 3-dehydroquinate dehydratase (NCBI) compare
b2913 serA -4.9 -5.8 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b3771 ilvD -4.9 -12.7 dihydroxy-acid dehydratase (NCBI) compare
b2021 hisC -4.9 -3.4 histidinol-phosphate aminotransferase (NCBI) compare
b0004 thrC -4.8 -7.4 threonine synthase (NCBI) compare
b3956 ppc -4.8 -16.1 phosphoenolpyruvate carboxylase (NCBI) compare
b2762 cysH -4.7 -7.2 phosphoadenosine phosphosulfate reductase (NCBI) compare
b0242 proB -4.7 -7.1 gamma-glutamyl kinase (NCBI) compare
b2557 purL -4.7 -7.1 phosphoribosylformylglycinamidine synthase (NCBI) compare
b4388 serB -4.6 -7.7 3-phosphoserine phosphatase (NCBI) compare
b4245 pyrB -4.6 -12.8 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b0386 proC -4.6 -8.3 pyrroline-5-carboxylate reductase (NCBI) compare
b3770 ilvE -4.6 -7.7 branched-chain amino acid aminotransferase (NCBI) compare
b4214 cysQ -4.6 -13.5 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b2414 cysK -4.6 -9.3 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b3829 metE -4.6 -17.7 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI) compare
b3957 argE -4.5 -16.0 acetylornithine deacetylase (NCBI) compare
b0522 purK -4.5 -6.8 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b3172 argG -4.5 -11.9 argininosuccinate synthase (NCBI) compare
b1062 pyrC -4.5 -8.0 dihydroorotase (NCBI) compare
b3960 argH -4.5 -10.4 argininosuccinate lyase (NCBI) compare
b0032 carA -4.4 -7.4 carbamoyl-phosphate synthase small subunit (NCBI) compare
b0003 thrB -4.2 -8.4 homoserine kinase (NCBI) compare
b2024 hisA -4.2 -2.8 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b3008 metC -4.1 -12.5 cystathionine beta-lyase (NCBI) compare
b0908 aroA -4.0 -9.4 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b2600 tyrA -4.0 -7.3 fused chorismate mutase T/prephenate dehydrogenase (NCBI) compare
b0052 pdxA -4.0 -12.5 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI) compare
b2818 argA -4.0 -8.9 N-acetylglutamate synthase (NCBI) compare
b3772 ilvA -4.0 -13.0 threonine dehydratase (NCBI) compare
b2026 hisI -4.0 -8.4 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI) compare
b3642 pyrE -3.8 -2.6 orotate phosphoribosyltransferase (NCBI) compare
b4013 metA -3.8 -6.8 homoserine O-succinyltransferase (NCBI) compare
b2476 purC -3.8 -3.0 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b0145 dksA -3.8 -3.7 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0049 apaH -3.8 -6.3 diadenosinetetraphosphatase (NCBI) compare
b3959 argB -3.7 -11.2 acetylglutamate kinase (RefSeq) compare
b2320 pdxB -3.7 -11.8 erythronate-4-phosphate dehydrogenase (NCBI) compare
b3958 argC -3.7 -15.4 N-acetyl-gamma-glutamyl-phosphate reductase (NCBI) compare
b0002 thrA -3.7 -16.6 bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI) compare
b2564 pdxJ -3.7 -6.1 pyridoxal phosphate biosynthetic protein (NCBI) compare
b2501 ppk -3.6 -12.3 polyphosphate kinase (NCBI) compare
b2499 purM -3.6 -6.0 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2750 cysC -3.6 -7.2 adenylylsulfate kinase (NCBI) compare
b3107 yhaL -3.5 -1.1 orf, hypothetical protein (VIMSS) compare
b1257 yciE -3.5 -2.0 hypothetical protein (NCBI) compare
b1281 pyrF -3.4 -2.4 orotidine 5'-phosphate decarboxylase (NCBI) compare
b0630 lipB -3.4 -2.4 protein of lipoate biosynthesis (VIMSS) compare
b0889 lrp -3.3 -4.4 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b3390 aroK -3.2 -3.8 shikimate kinase I (NCBI) compare
b2763 cysI -3.2 -10.4 sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI) compare
b0726 sucA -3.1 -3.1 alpha-ketoglutarate decarboxylase (NCBI) compare
b4603 rseX -3.1 -1.0 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b2764 cysJ -3.1 -9.4 sulfite reductase, alpha subunit, flavoprotein (NCBI) compare
b3761 trpT -3.0 -1.4 tRNA-Trp (NCBI) compare
b0720 gltA -3.0 -5.7 citrate synthase (NCBI) compare
b2839 lysR -3.0 -6.2 DNA-binding transcriptional dual regulator (NCBI) compare
b0142 folK -3.0 -1.0 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1848 yebG -3.0 -1.0 conserved protein regulated by LexA (NCBI) compare
b4407 thiS -2.9 -2.1 sulfur carrier protein ThiS (NCBI) compare
b4025 pgi -2.9 -9.7 glucose-6-phosphate isomerase (NCBI) compare
b3939 metB -2.8 -12.9 cystathionine gamma-synthase (NCBI) compare
b3916 pfkA -2.8 -3.3 6-phosphofructokinase (NCBI) compare
b3368 cysG -2.8 -16.0 fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI) compare
b2479 gcvR -2.8 -5.4 transcriptional regulation of gcv operon (VIMSS) compare
b0750 nadA -2.8 -6.7 quinolinate synthetase (NCBI) compare
b0928 aspC -2.8 -9.0 aspartate aminotransferase, PLP-dependent (NCBI) compare
b0592 fepB -2.6 -9.7 iron-enterobactin transporter subunit (NCBI) compare
b2424 cysU -2.6 -3.1 sulfate/thiosulfate transporter subunit (NCBI) compare
b1273 yciN -2.6 -2.3 hypothetical protein (NCBI) compare
b0585 fes -2.6 -7.1 enterobactin/ferric enterobactin esterase (NCBI) compare
b3773 ilvY -2.5 -9.9 DNA-binding transcriptional dual regulator (NCBI) compare
b1263 trpD -2.5 -2.4 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b0683 fur -2.5 -1.8 ferric uptake regulator (NCBI) compare
b0590 fepD -2.5 -7.1 iron-enterobactin transporter subunit (NCBI) compare
b1192 ldcA -2.5 -7.2 L,D-carboxypeptidase A (NCBI) compare
b1183 umuD -2.5 -1.3 DNA polymerase V, subunit D (NCBI) compare
b1217 chaB -2.5 -1.7 cation transport regulator (NCBI) compare
b1426 ydcH -2.4 -1.4 orf, hypothetical protein (VIMSS) compare
b1850 eda -2.4 -1.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1262 trpC -2.4 -3.4 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b4699 fnrS -2.3 -0.7 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b2500 purN -2.3 -5.9 phosphoribosylglycinamide formyltransferase (NCBI) compare
b2318 truA -2.3 -4.1 tRNA pseudouridine synthase A (NCBI) compare
b2830 nudH -2.2 -2.6 dinucleoside polyphosphate hydrolase (NCBI) compare
b2450 yffS -2.2 -1.9 CPZ-55 prophage; predicted protein (RefSeq) compare
b2620 smpB -2.2 -2.2 SsrA-binding protein (NCBI) compare
b2599 pheA -2.2 -3.9 fused chorismate mutase P/prephenate dehydratase (NCBI) compare
b1151 ymfO -2.1 -3.4 e14 prophage; conserved protein (RefSeq) compare
b4279 yjhB -2.1 -4.0 putative transport protein (VIMSS) compare
b0589 fepG -2.1 -6.6 iron-enterobactin transporter subunit (NCBI) compare
b4177 purA -2.1 -2.0 adenylosuccinate synthetase (NCBI) compare
b0114 aceE -2.1 -2.8 pyruvate dehydrogenase subunit E1 (NCBI) compare
b4246 pyrL -2.1 -1.8 pyrBI operon leader peptide (NCBI) compare
b2058 wcaB -2.1 -0.6 predicted acyl transferase (NCBI) compare
b0623 cspE -2.0 -1.6 cold shock protein E (NCBI) compare
b0437 clpP -2.0 -8.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1685 ydiH -2.0 -0.6 predicted protein (RefSeq) compare
b0888 trxB -2.0 -4.2 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b3811 xerC -2.0 -3.8 site-specific tyrosine recombinase XerC (NCBI) compare
b0588 fepC -2.0 -8.5 iron-enterobactin transporter subunit (NCBI) compare
b2313 cvpA -2.0 -4.8 membrane protein required for colicin V production (NCBI) compare
b0990 cspG -1.9 -1.8 DNA-binding transcriptional regulator (NCBI) compare
b3204 ptsN -1.9 -5.6 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1290 sapF -1.9 -2.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0439 lon -1.9 -6.0 DNA-binding ATP-dependent protease La (NCBI) compare
b0109 nadC -1.9 -6.6 nicotinate-nucleotide pyrophosphorylase (NCBI) compare
b4533 ynfO -1.8 -1.6 Qin prophage; predicted protein (NCBI) compare
b3429 glgA -1.8 -10.0 glycogen synthase (NCBI) compare
b1839 yebY -1.8 -2.6 hypothetical protein (NCBI) compare
b2752 cysD -1.8 -4.2 sulfate adenylyltransferase subunit 2 (NCBI) compare
b3935 priA -1.8 -1.7 primosome assembly protein PriA (NCBI) compare
b3442 yhhZ -1.8 -2.7 hypothetical protein (NCBI) compare
b0200 gmhB -1.7 -2.3 hypothetical protein (NCBI) compare
b3870 glnA -1.7 -2.4 glutamine synthetase (NCBI) compare
b3992 thiF -1.7 -6.6 thiamin biosynthesis, thiazole moiety (VIMSS) compare
b3462 ftsX -1.7 -5.5 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0624 crcB -1.7 -2.7 camphor resistance protein CrcB (NCBI) compare
b2751 cysN -1.7 -5.6 sulfate adenylyltransferase subunit 1 (NCBI) compare
b0118 acnB -1.7 -3.9 aconitate hydratase (NCBI) compare
b3650 spoT -1.7 -4.9 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b4515 ybgT -1.6 -1.0 hypothetical protein (NCBI) compare
b2659 b2659 -1.6 -1.5 orf, hypothetical protein (VIMSS) compare
b3463 ftsE -1.6 -4.7 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2962 yggX -1.6 -2.3 hypothetical protein (NCBI) compare
b1486 ddpB -1.6 -2.4 D-ala-D-ala transporter subunit (NCBI) compare
b3994 thiC -1.5 -9.8 thiamine biosynthesis protein ThiC (NCBI) compare
b3068 ygjF -1.5 -1.7 G/U mismatch-specific DNA glycosylase (NCBI) compare
b3386 rpe -1.5 -1.0 ribulose-phosphate 3-epimerase (NCBI) compare
b0113 pdhR -1.5 -4.8 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b1847 yebF -1.5 -1.9 predicted protein (RefSeq) compare
b2456 cchB -1.5 -2.2 predicted carboxysome structural protein, ethanolamine utilization protein (NCBI) compare
b2188 yejM -1.5 -6.6 predicted hydrolase, inner membrane (NCBI) compare
b0767 ybhE -1.5 -3.5 6-phosphogluconolactonase (NCBI) compare
b3121 yhaC -1.5 -2.3 hypothetical protein (NCBI) compare
b3082 ygjM -1.4 -2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b3236 mdh -1.4 -5.6 malate dehydrogenase (NCBI) compare
b3993 thiE -1.4 -5.5 thiamine-phosphate pyrophosphorylase (NCBI) compare
b3572 avtA -1.4 -7.5 valine--pyruvate transaminase (NCBI) compare
b0740 tolB -1.4 -5.8 translocation protein TolB precursor (NCBI) compare
b1936 intG -1.4 -1.3 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2911 ssrS -1.4 -0.6 6S regulatory RNA (NCBI) compare
b3483 yhhH -1.4 -1.6 orf, hypothetical protein (VIMSS) compare
b1264 trpE -1.3 -3.5 anthranilate synthase component I (NCBI) compare
b3991 thiG -1.3 -5.6 thiamin biosynthesis, thiazole moiety (VIMSS) compare
b1861 ruvA -1.3 -2.2 Holliday junction DNA helicase motor protein (NCBI) compare
b3990 thiH -1.3 -4.9 thiamine biosynthesis protein ThiH (NCBI) compare
b1013 ycdC -1.3 -1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b1545 pinQ -1.3 -1.4 Qin prophage; predicted site-specific recombinase (NCBI) compare
b3290 trkA -1.3 -3.3 potassium transporter peripheral membrane component (NCBI) compare
b2894 xerD -1.2 -1.2 site-specific tyrosine recombinase XerD (NCBI) compare
b3725 pstB -1.2 -2.9 phosphate transporter subunit (NCBI) compare
b0603 ybdO -1.2 -2.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b3781 trxA -1.2 -3.0 thioredoxin 1 (VIMSS) compare
b2601 aroF -1.2 -3.9 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tyrosine-repressible (NCBI) compare
b4441 glmY -1.2 -1.1 sRNA activator of glmS mRNA (RefSeq) compare
b3052 rfaE -1.2 -5.2 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b4066 yjcF -1.2 -2.3 hypothetical protein (NCBI) compare
b3769 ilvM -1.2 -2.9 acetolactate synthase II, small subunit (NCBI) compare
b1523 yneG -1.2 -1.5 hypothetical protein (NCBI) compare
b2574 nadB -1.2 -5.9 L-aspartate oxidase (NCBI) compare
b2103 thiD -1.2 -4.1 phosphomethylpyrimidine kinase (NCBI) compare
b4411 ecnB -1.1 -2.0 entericidin B membrane lipoprotein (NCBI) compare
b1432 insQ -1.1 -4.2 IS609 transposase B (RefSeq) compare
b3619 rfaD -1.1 -4.1 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1708 nlpC -1.1 -2.4 predicted lipoprotein (NCBI) compare
b0741 pal -1.1 -3.4 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1503 ydeR -1.1 -4.2 predicted fimbrial-like adhesin protein (NCBI) compare
b1271 yciK -1.1 -2.4 short chain dehydrogenase (NCBI) compare
b1148 ymfM -1.1 -2.1 e14 prophage; predicted protein (NCBI) compare
b1857 yebL -1.1 -3.7 putative adhesin (VIMSS) compare
b0222 lpcA -1.1 -1.9 phosphoheptose isomerase (NCBI) compare
b1346 ydaQ -1.1 -2.4 orf, hypothetical protein (VIMSS) compare
b1164 ycgZ -1.1 -1.9 hypothetical protein (NCBI) compare


Specific Phenotypes

For 12 genes in this experiment