Experiment set3IT032 for Escherichia coli BW25113

Compare to:

deuterated glucose in H2O

200 most detrimental genes:

  gene name fitness t score description  
b3979 thrT +2.0 2.0 tRNA-Thr (NCBI) compare
b3456 livM +1.8 10.9 leucine/isoleucine/valine transporter subunit (NCBI) compare
b3460 livJ +1.7 9.8 leucine/isoleucine/valine transporter subunit (NCBI) compare
b4172 hfq +1.7 3.5 RNA-binding protein Hfq (NCBI) compare
b2808 gcvA +1.6 7.0 DNA-binding transcriptional dual regulator (NCBI) compare
b3454 livF +1.6 7.6 ATP-binding component of leucine transport (VIMSS) compare
b2904 gcvH +1.5 5.3 glycine cleavage system protein H (NCBI) compare
b0496 ybbP +1.5 9.7 predicted inner membrane protein (NCBI) compare
b1231 tyrT +1.5 0.4 tRNA-Tyr (NCBI) compare
b3455 livG +1.4 5.5 leucine/isoleucine/valine transporter subunit (NCBI) compare
b4443 gcvB +1.4 3.5 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b4094 phnN +1.3 5.1 ribose 1,5-bisphosphokinase (NCBI) compare
b4096 phnL +1.3 4.1 carbon-phosphorus lyase complex subunit (NCBI) compare
b4598 yncL +1.3 3.5 hypothetical protein (RefSeq) compare
b4097 phnK +1.3 5.1 carbon-phosphorus lyase complex subunit (NCBI) compare
b4631 ykgQ +1.3 1.7 no description compare
b3457 livH +1.2 8.0 leucine/isoleucine/valine transporter subunit (NCBI) compare
b2903 gcvP +1.2 7.8 glycine dehydrogenase (NCBI) compare
b1007 b1007 +1.2 2.5 orf, hypothetical protein (VIMSS) compare
b3261 fis +1.2 5.4 DNA-binding protein Fis (NCBI) compare
b2905 gcvT +1.2 6.8 glycine cleavage system aminomethyltransferase T (NCBI) compare
b0494 tesA +1.2 2.9 multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI) compare
b3458 livK +1.2 5.7 leucine transporter subunit (NCBI) compare
b1380 ldhA +1.2 2.4 D-lactate dehydrogenase (NCBI) compare
b4239 treC +1.2 5.1 trehalose-6-P hydrolase (NCBI) compare
b1360 ydaV +1.2 4.1 Rac prophage; predicted DNA replication protein (NCBI) compare
b2688 gshA +1.1 6.4 glutamate--cysteine ligase (NCBI) compare
b0497 rhsD +1.1 8.5 rhsD element protein (NCBI) compare
b1332 ynaJ +1.1 1.5 predicted inner membrane protein (NCBI) compare
b0685 ybfE +1.1 2.4 orf, hypothetical protein (VIMSS) compare
b1150 ymfR +1.1 1.6 e14 prophage; predicted protein (NCBI) compare
b3229 sspA +1.1 4.5 stringent starvation protein A (NCBI) compare
b1842 holE +1.0 2.0 DNA polymerase III, theta subunit (NCBI) compare
b3523 yhjE +1.0 7.0 predicted transporter (NCBI) compare
b1438 ydcQ +1.0 3.0 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0505 allA +1.0 1.6 ureidoglycolate hydrolase (NCBI) compare
b4627 ykfL +1.0 4.1 no description compare
b4095 phnM +1.0 4.7 carbon-phosphorus lyase complex subunit (NCBI) compare
b0271 yagH +1.0 3.7 CP4-6 prophage; predicted xylosidase/arabinosidase (NCBI) compare
b4098 phnJ +1.0 5.3 carbon-phosphorus lyase complex subunit (NCBI) compare
b0493 ybbO +1.0 3.0 short chain dehydrogenase (NCBI) compare
b2236 yfaE +1.0 3.1 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1279 yciS +0.9 0.3 conserved inner membrane protein (NCBI) compare
b1085 b1085 +0.9 1.6 orf, hypothetical protein (VIMSS) compare
b4529 ydbJ +0.9 1.3 hypothetical protein (NCBI) compare
b4676 yoaK +0.9 2.1 expressed protein (RefSeq) compare
b4595 yciY +0.9 1.9 hypothetical protein (RefSeq) compare
b2784 relA +0.9 5.1 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b1514 lsrC +0.9 1.5 AI2 transporter (NCBI) compare
b4695 ykgT +0.9 1.6 no description compare
b2017 yefM +0.9 2.0 orf, hypothetical protein (VIMSS) compare
b2007 yeeX +0.9 2.4 conserved protein (RefSeq) compare
b4090 rpiB +0.9 3.7 ribose-5-phosphate isomerase B (NCBI) compare
b1686 ydiI +0.9 2.7 hypothetical protein (NCBI) compare
b1942 fliJ +0.9 1.1 flagellar biosynthesis chaperone (NCBI) compare
b1550 b1550 +0.9 1.6 orf, hypothetical protein (VIMSS) compare
b1778 yeaA +0.9 3.4 methionine sulfoxide reductase B (NCBI) compare
b2939 yqgB +0.9 2.2 predicted protein (RefSeq) compare
b4163 glyV +0.8 2.0 tRNA-Gly (NCBI) compare
b0270 yagG +0.8 2.5 CP4-6 prophage; predicted sugar transporter (NCBI) compare
b1370 insH-5 +0.8 1.3 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b2317 dedA +0.8 2.8 conserved inner membrane protein (NCBI) compare
b1307 pspD +0.8 1.6 peripheral inner membrane phage-shock protein (NCBI) compare
b0272 yagI +0.8 3.3 CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b4487 yjdP +0.8 3.5 hypothetical protein (NCBI) compare
b0705 ybfL +0.8 3.1 putative receptor protein (VIMSS) compare
b4099 phnI +0.8 3.4 carbon-phosphorus lyase complex subunit (NCBI) compare
b4087 alsA +0.8 5.1 fused D-allose transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2012 yeeD +0.8 3.0 hypothetical protein (NCBI) compare
b0495 ybbA +0.8 2.3 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4092 phnP +0.8 4.0 carbon-phosphorus lyase complex accessory protein (NCBI) compare
b2673 nrdH +0.8 1.2 glutaredoxin-like protein (NCBI) compare
b4577 sgrS +0.8 2.1 small antisense RNA (NCBI) compare
b0504 ybbS +0.8 2.8 DNA-binding transcriptional activator of the allD operon (NCBI) compare
b0225 yafQ +0.8 3.9 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1141 ymfG +0.8 1.4 e14 prophage; predicted excisionase (NCBI) compare
b2586 yfiM +0.8 2.0 predicted protein (RefSeq) compare
b4647 mokA +0.8 1.6 no description compare
b2814 metZ +0.8 1.6 tRNA-Met (NCBI) compare
b0499 ylbH +0.8 5.2 conserved protein, rhs-like (RefSeq) compare
b2829 ptsP +0.8 4.9 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1427 rimL +0.8 2.7 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b4402 yjjY +0.8 1.5 hypothetical protein (NCBI) compare
b2343 b2343 +0.8 1.3 orf, hypothetical protein (VIMSS) compare
b2597 yfiA +0.8 1.8 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b1760 ynjH +0.7 3.1 hypothetical protein (NCBI) compare
b1727 yniC +0.7 1.5 predicted hydrolase (NCBI) compare
b2287 nuoB +0.7 2.5 NADH dehydrogenase subunit B (NCBI) compare
b0491 ybbM +0.7 4.0 putative metal resistance protein (VIMSS) compare
b1397 paaJ +0.7 3.7 acetyl-CoA acetyltransferase (NCBI) compare
b2248 yfaX +0.7 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b2741 rpoS +0.7 2.4 RNA polymerase sigma factor (NCBI) compare
b1010 ycdK +0.7 2.5 hypothetical protein (NCBI) compare
b0822 ybiV +0.7 2.3 predicted hydrolase (NCBI) compare
b1531 marA +0.7 1.3 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b3500 gor +0.7 1.7 glutathione reductase (NCBI) compare
b0487 cueR +0.7 2.5 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b1827 kdgR +0.7 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b0273 argF +0.7 2.6 CP4-6 prophage; ornithine carbamoyltransferase 2, chain F (NCBI) compare
b0059 hepA +0.7 4.9 ATP-dependent helicase HepA (NCBI) compare
b2061 wzb +0.7 1.8 protein-tyrosine phosphatase (NCBI) compare
b3670 ilvN +0.7 3.3 acetolactate synthase small subunit (NCBI) compare
b2531 iscR +0.7 1.4 DNA-binding transcriptional repressor (NCBI) compare
b4202 rpsR +0.7 1.8 30S ribosomal protein S18 (NCBI) compare
b1104 ycfL +0.7 2.3 hypothetical protein (NCBI) compare
b3199 yrbK +0.7 1.6 hypothetical protein (NCBI) compare
b4557 yidD +0.7 3.2 hypothetical protein (NCBI) compare
b4391 yjjK +0.7 4.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1642 slyA +0.7 1.1 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0051 ksgA +0.7 3.5 dimethyladenosine transferase (NCBI) compare
b0236 prfH +0.7 3.7 peptide chain release factor 2 (NCBI) compare
b0249 ykfF +0.7 0.8 CP4-6 prophage; predicted protein (NCBI) compare
b0404 yajB +0.7 2.4 hypothetical protein (NCBI) compare
b1317 ycjU +0.7 2.2 predicted beta-phosphoglucomutase (NCBI) compare
b1955 yedP +0.7 2.5 hypothetical protein (NCBI) compare
b2953 yggU +0.7 1.2 orf, hypothetical protein (VIMSS) compare
b2421 cysM +0.6 2.0 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b1008 ycdI +0.6 1.3 hypothetical protein (NCBI) compare
b4408 csrB +0.6 2.4 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b1882 cheY +0.6 1.2 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b4093 phnO +0.6 1.7 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2490 b2490 +0.6 1.2 putative protein processing element (VIMSS) compare
b2390 ypeC +0.6 2.3 hypothetical protein (NCBI) compare
b1095 fabF +0.6 3.5 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b2128 yehW +0.6 1.7 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1394 paaG +0.6 2.0 enoyl-CoA hydratase (NCBI) compare
b0872 hcr +0.6 3.3 HCP oxidoreductase, NADH-dependent (NCBI) compare
b1506 yneL +0.6 2.2 predicted transcriptional regulator (NCBI) compare
b1296 ycjJ +0.6 2.1 putative amino acid/amine transport protein (VIMSS) compare
b1422 ydcI +0.6 2.4 putative transcriptional regulator LYSR-type (VIMSS) compare
b1960 vsr +0.6 2.2 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b4493 gapC +0.6 1.6 no description compare
b1605 ydgI +0.6 2.5 predicted arginine/ornithine antiporter transporter (NCBI) compare
b0490 ybbL +0.6 3.6 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2297 pta +0.6 1.4 phosphate acetyltransferase (NCBI) compare
b2705 srlD +0.6 2.7 3-ketoacyl-(acyl-carrier-protein) reductase (NCBI) compare
b0268 yagE +0.6 1.6 2-keto-3-deoxy gluconate (KDG) aldolase; CP4-6 prophage (NCBI) compare
b2199 ccmC +0.6 1.9 heme exporter subunit (NCBI) compare
b2419 yfeK +0.6 1.2 hypothetical protein (NCBI) compare
b1946 fliN +0.6 2.0 flagellar motor switch protein (NCBI) compare
b4089 rpiR +0.6 4.3 transcriptional repressor of rpiB expression (VIMSS) compare
b3113 yhaR +0.6 2.1 orf, hypothetical protein (VIMSS) compare
b4477 dgoA +0.6 2.3 2-dehydro-3-deoxy-6-phosphogalactonate aldolase (NCBI) compare
b0810 glnP +0.6 3.0 glutamine ABC transporter permease protein (NCBI) compare
b1475 fdnH +0.6 2.2 formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI) compare
b2432 yfeY +0.6 1.1 hypothetical protein (NCBI) compare
b4088 alsB +0.6 3.9 D-allose transporter subunit (NCBI) compare
b0492 ybbN +0.6 3.3 putative thioredoxin-like protein (VIMSS) compare
b4444 rygA +0.6 1.4 unknown RNA (NCBI) compare
b1011 b1011 +0.6 1.8 putative synthetase (VIMSS) compare
b0864 artP +0.6 1.4 arginine transporter subunit (NCBI) compare
b0187 yaeR +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b0627 tatE +0.6 1.0 twin arginine translocase protein E (NCBI) compare
b4608 ryfC +0.6 2.0 Novel sRNA, function unknown (RefSeq) compare
b3863 polA +0.6 0.7 DNA polymerase I (NCBI) compare
b4416 rybA +0.6 0.3 unknown RNA (NCBI) compare
b2947 gshB +0.6 2.0 glutathione synthetase (NCBI) compare
b2204 napH +0.6 2.4 quinol dehydrogenase membrane component (NCBI) compare
b2051 wcaH +0.6 1.0 GDP-mannose mannosyl hydrolase (VIMSS) compare
b3866 yihI +0.6 2.2 hypothetical protein (NCBI) compare
b1072 flgA +0.6 1.8 flagellar basal body P-ring biosynthesis protein A (NCBI) compare
b4641 yoeH +0.6 2.2 no description compare
b0191 yaeJ +0.6 1.6 hypothetical protein (NCBI) compare
b1733 chbG +0.6 1.4 hypothetical protein (NCBI) compare
b4520 ymgF +0.6 1.6 hypothetical protein (NCBI) compare
b2209 eco +0.6 1.9 ecotin precursor (NCBI) compare
b1086 rluC +0.6 2.8 23S rRNA pseudouridylate synthase (NCBI) compare
b1487 ddpA +0.6 2.0 D-ala-D-a la transporter subunit (NCBI) compare
b1560 ydfU +0.6 2.2 Qin prophage; predicted protein (RefSeq) compare
b4426 isrA +0.6 1.3 unknown RNA (NCBI) compare
b2114 metG +0.6 1.0 methionyl-tRNA synthetase (NCBI) compare
b0966 hspQ +0.6 1.8 DNA-binding protein, hemimethylated (RefSeq) compare
b2314 dedD +0.6 1.4 putative lipoprotein (VIMSS) compare
b3582 sgbU +0.5 1.3 probable 3-hexulose-6-phosphate isomerase (VIMSS) compare
b0226 dinJ +0.5 1.1 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b4101 phnG +0.5 1.3 carbon-phosphorus lyase complex subunit (NCBI) compare
b2237 inaA +0.5 1.5 hypothetical protein (NCBI) compare
b2225 yfaP +0.5 2.9 hypothetical protein (NCBI) compare
b3574 yiaJ +0.5 2.5 predicted DNA-binding transcriptional repressor (NCBI) compare
b0520 ylbF +0.5 2.5 hypothetical protein (NCBI) compare
b1677 lpp +0.5 1.5 murein lipoprotein (NCBI) compare
b1989 asnV +0.5 1.3 tRNA-Asn (NCBI) compare
b2617 smpA +0.5 1.7 small membrane protein A (VIMSS) compare
b0339 cynT +0.5 1.4 carbonic anhydrase (NCBI) compare
b2141 yohJ +0.5 1.5 hypothetical protein (NCBI) compare
b1953 b1953 +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b2428 yfeU +0.5 1.9 N-acetylmuramic acid-6-phosphate etherase (NCBI) compare
b2700 ygaD +0.5 1.8 competence damage-inducible protein A (NCBI) compare
b0488 ybbJ +0.5 1.7 conserved inner membrane protein (RefSeq) compare
b2229 b2229 +0.5 0.9 orf, hypothetical protein (VIMSS) compare
b0486 ybaT +0.5 2.2 predicted transporter (NCBI) compare
b3676 yidH +0.5 2.1 conserved inner membrane protein (NCBI) compare
b2951 yggS +0.5 2.0 predicted enzyme (NCBI) compare
b2768 ygcP +0.5 1.2 predicted anti-terminator regulatory protein (NCBI) compare
b1355 b1355 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b4675 yoaJ +0.5 1.3 expressed protein (RefSeq) compare
b2944 sprT +0.5 2.9 hypothetical protein (NCBI) compare
b0260 ykfD +0.5 2.5 putative amino acid/amine transport protein (VIMSS) compare
b1593 b1593 +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b0717 ybgP +0.5 1.9 predicted assembly protein (NCBI) compare


Specific Phenotypes

For 12 genes in this experiment