Experiment set3IT032 for Escherichia coli BW25113

deuterated glucose in H2O

Group: protonated/deuterated
Media: M9
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Garth on dec_2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 12 genes in this experiment

SEED Subsystems

Subsystem	#Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1)	5
Alanine biosynthesis	1
Alkylphosphonate utilization	1
Branched-Chain Amino Acid Biosynthesis	1
Pyruvate Alanine Serine Interconversions	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Purine metabolism
• Valine, leucine and isoleucine biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine biosynthesis I	2	2	1
superpathway of L-alanine biosynthesis	4	4	1
guanosine ribonucleotides de novo biosynthesis	4	4	1
methylphosphonate degradation I	5	5	1
dZTP biosynthesis	5	3	1
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
glyphosate degradation III	7	4	1
(aminomethyl)phosphonate degradation	8	8	1
superpathway of guanosine nucleotides de novo biosynthesis II	8	8	1
superpathway of purine nucleotide salvage	14	13	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1
superpathway of purine nucleotides de novo biosynthesis II	26	26	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	46	1