Experiment set3IT031 for Escherichia coli BW25113

deuterated glucose in H2O

Group: protonated/deuterated
Media: M9
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Garth on dec_2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 3 genes in this experiment

SEED Subsystems

Subsystem	#Specific
De Novo Pyrimidine Synthesis	1
Pentose phosphate pathway	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Pyrimidine metabolism
• Alanine and aspartate metabolism
• Glutathione metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pentose phosphate pathway (oxidative branch) I	3	3	1
thiazole component of thiamine diphosphate biosynthesis I	6	6	1
UMP biosynthesis I	6	6	1
UMP biosynthesis II	6	6	1
UMP biosynthesis III	6	5	1
pentose phosphate pathway	8	8	1
superpathway of pyrimidine ribonucleotides de novo biosynthesis	9	9	1
superpathway of thiamine diphosphate biosynthesis I	10	10	1
superpathway of glucose and xylose degradation	17	17	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	46	1