Experiment set3IT022 for Agrobacterium fabrum C58

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4-Aminobutyric acid nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Succinate_noNitrogen + 4-Aminobutyric acid (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 11/20/20
Media components: 10 mM Sodium succinate dibasic hexahydrate, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 10 genes in this experiment

For nitrogen source 4-Aminobutyric acid in Agrobacterium fabrum C58

For nitrogen source 4-Aminobutyric acid across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 5
Pyruvate Alanine Serine Interconversions 2
Alanine biosynthesis 1
Biotin biosynthesis 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine degradation I 2 2 2
alanine racemization 1 1 1
anaerobic energy metabolism (invertebrates, cytosol) 7 5 1
ansatrienin biosynthesis 9 1 1
cyclosporin A biosynthesis 15 2 1
superpathway of anaerobic energy metabolism (invertebrates) 17 13 1