Experiment set3IT021 for Pseudomonas simiae WCS417

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Sodium octanoate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Sodium octanoate (20 mM), pH=7
Culturing: fluoroDangl_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 2/18/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 30 genes in this experiment

For carbon source Sodium octanoate in Pseudomonas simiae WCS417

For carbon source Sodium octanoate across organisms

SEED Subsystems

Subsystem #Specific
Lipid A modifications 2
Molybdenum cofactor biosynthesis 2
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 2
Ammonia assimilation 1
Biotin biosynthesis 1
Chorismate Synthesis 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
Entner-Doudoroff Pathway 1
Fermentations: Mixed acid 1
Formate hydrogenase 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Methylglyoxal Metabolism 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
RNA processing and degradation, bacterial 1
Siderophore Pyoverdine 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
ethanol degradation I 2 2 2
bis(guanylyl molybdenum cofactor) biosynthesis 2 2 2
bis(guanylyl tungstenpterin) cofactor biosynthesis 1 1 1
long-chain fatty acid activation 1 1 1
acetaldehyde biosynthesis I 1 1 1
guanylyl molybdenum cofactor biosynthesis 1 1 1
formate oxidation to CO2 1 1 1
phytol degradation 4 3 3
ethanol degradation II 3 3 2
pyruvate fermentation to ethanol III 3 2 2
pyruvate fermentation to ethanol I 3 2 2
putrescine degradation V 2 2 1
3-dehydroquinate biosynthesis I 2 2 1
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
L-threonine degradation IV 2 2 1
3-methyl-branched fatty acid α-oxidation 6 3 3
linoleate biosynthesis II (animals) 2 1 1
phenylethylamine degradation I 2 1 1
ethylene glycol degradation 2 1 1
putrescine degradation I 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
phenylethanol degradation 2 1 1
phenylethylamine degradation II 2 1 1
pyruvate fermentation to ethanol II 2 1 1
ethanolamine utilization 5 5 2
acetylene degradation (anaerobic) 5 4 2
octane oxidation 5 4 2
sphingosine and sphingosine-1-phosphate metabolism 10 4 4
fatty acid salvage 6 6 2
ethanol degradation IV 3 3 1
2-aminoethylphosphonate degradation I 3 3 1
L-isoleucine degradation II 3 2 1
L-valine degradation II 3 2 1
L-leucine degradation III 3 2 1
ethanol degradation III 3 2 1
hypotaurine degradation 3 2 1
2-deoxy-D-ribose degradation I 3 2 1
L-phenylalanine degradation II (anaerobic) 3 2 1
putrescine degradation IV 3 2 1
histamine degradation 3 1 1
styrene degradation 3 1 1
alkane biosynthesis II 3 1 1
oleate biosynthesis I (plants) 3 1 1
L-methionine degradation III 3 1 1
sulfoacetaldehyde degradation IV 3 1 1
2-deoxy-α-D-ribose 1-phosphate degradation 3 1 1
2-hydroxypenta-2,4-dienoate degradation 3 1 1
noradrenaline and adrenaline degradation 13 4 4
serotonin degradation 7 3 2
ceramide degradation by α-oxidation 7 2 2
fatty acid α-oxidation I (plants) 4 2 1
putrescine degradation III 4 2 1
L-phenylalanine degradation III 4 2 1
D-arabinose degradation II 4 2 1
L-tyrosine degradation III 4 2 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 4
salidroside biosynthesis 4 1 1
oxalate degradation VI 4 1 1
L-tryptophan degradation X (mammalian, via tryptamine) 4 1 1
phosphatidylcholine acyl editing 4 1 1
wax esters biosynthesis II 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 5 2
sporopollenin precursors biosynthesis 18 4 4
adipate degradation 5 5 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
mitochondrial NADPH production (yeast) 5 4 1
(S)-propane-1,2-diol degradation 5 2 1
phenylethanol biosynthesis 5 1 1
dopamine degradation 5 1 1
oxalate degradation III 5 1 1
catechol degradation I (meta-cleavage pathway) 5 1 1
mixed acid fermentation 16 12 3
stearate biosynthesis II (bacteria and plants) 6 5 1
stearate biosynthesis IV 6 4 1
superpathway of pyrimidine deoxyribonucleosides degradation 6 3 1
6-gingerol analog biosynthesis (engineered) 6 2 1
stearate biosynthesis I (animals) 6 1 1
alkane oxidation 6 1 1
triethylamine degradation 6 1 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 5 2
chorismate biosynthesis I 7 7 1
3-methylbutanol biosynthesis (engineered) 7 6 1
superpathway of purine deoxyribonucleosides degradation 7 5 1
superpathway of glycol metabolism and degradation 7 5 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
catechol degradation II (meta-cleavage pathway) 7 2 1
limonene degradation IV (anaerobic) 7 1 1
toluene degradation I (aerobic) (via o-cresol) 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
toluene degradation V (aerobic) (via toluene-cis-diol) 7 1 1
capsaicin biosynthesis 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
partial TCA cycle (obligate autotrophs) 8 8 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 7 1
2-deoxy-D-ribose degradation II 8 7 1
nitrogen remobilization from senescing leaves 8 6 1
superpathway of ornithine degradation 8 6 1
butanol and isobutanol biosynthesis (engineered) 8 3 1
3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation 8 2 1
aromatic biogenic amine degradation (bacteria) 8 2 1
p-cumate degradation 8 1 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 2
oleate β-oxidation 35 30 4
heterolactic fermentation 18 14 2
Entner-Doudoroff pathway II (non-phosphorylative) 9 6 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 3 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 2
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of L-phenylalanine biosynthesis 10 10 1
meta cleavage pathway of aromatic compounds 10 3 1
suberin monomers biosynthesis 20 2 2
superpathway of fatty acid biosynthesis II (plant) 43 38 4
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 8 1
superpathway of N-acetylneuraminate degradation 22 15 2
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 1
superpathway of phenylethylamine degradation 11 4 1
p-cymene degradation 11 1 1
superpathway of C1 compounds oxidation to CO2 12 5 1
L-tryptophan degradation XII (Geobacillus) 12 4 1
L-tryptophan degradation IX 12 4 1
naphthalene degradation to acetyl-CoA 12 2 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of L-arginine and L-ornithine degradation 13 10 1
formaldehyde assimilation I (serine pathway) 13 6 1
toluene degradation IV (aerobic) (via catechol) 13 4 1
L-tryptophan degradation V (side chain pathway) 13 1 1
2-methyl-branched fatty acid β-oxidation 14 11 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 1
purine nucleobases degradation I (anaerobic) 15 6 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
cutin biosynthesis 16 1 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of L-threonine metabolism 18 12 1
mandelate degradation to acetyl-CoA 18 9 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
superpathway of anaerobic sucrose degradation 19 14 1
purine nucleobases degradation II (anaerobic) 24 16 1
ethene biosynthesis V (engineered) 25 18 1
superpathway of fatty acids biosynthesis (E. coli) 53 49 2
anaerobic aromatic compound degradation (Thauera aromatica) 27 3 1
palmitate biosynthesis III 29 21 1
superpathway of aerobic toluene degradation 30 12 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 21 1
superpathway of pentose and pentitol degradation 42 16 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 15 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 20 1
superpathway of chorismate metabolism 59 44 1