Experiment set3IT020 for Agrobacterium fabrum C58

Beta-alanine nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Succinate_noNitrogen + Beta-alanine (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 11/20/20
Media components: 10 mM Sodium succinate dibasic hexahydrate, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 12 genes in this experiment

For nitrogen source Beta-alanine in Agrobacterium fabrum C58

For nitrogen source Beta-alanine across organisms

SEED Subsystems

Subsystem	#Specific
Pyruvate Alanine Serine Interconversions	3
Alanine biosynthesis	1
Respiratory dehydrogenases 1	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Alanine and aspartate metabolism
• Valine, leucine and isoleucine degradation
• Phenylalanine metabolism
• beta-Alanine metabolism
• D-Alanine metabolism
• Propanoate metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine degradation I	2	2	2
alanine racemization	1	1	1
β-alanine degradation II	2	2	1
β-alanine biosynthesis II	6	3	1
anaerobic energy metabolism (invertebrates, cytosol)	7	5	1
ansatrienin biosynthesis	9	1	1
superpathway of coenzyme A biosynthesis II (plants)	10	7	1
cyclosporin A biosynthesis	15	2	1
superpathway of anaerobic energy metabolism (invertebrates)	17	13	1