Experiment set3IT019 for Pontibacter actiniarum KMM 6156, DSM 19842

marine broth with Tetracycline hydrochloride 0.006 mg/ml

Group: stress
Media: marine_broth_2216 + Tetracycline hydrochloride (0.006 mg/ml)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.3 generations
By: Adam on 8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1736 B2

Specific Phenotypes

For 2 genes in this experiment

For stress Tetracycline hydrochloride in Pontibacter actiniarum KMM 6156, DSM 19842

For stress Tetracycline hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Entner-Doudoroff Pathway	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Methylglyoxal Metabolism	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Ascorbate and aldarate metabolism
• Fatty acid metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine degradation
• Lysine degradation
• Arginine and proline metabolism
• Histidine metabolism
• Tryptophan metabolism
• beta-Alanine metabolism
• Glycerolipid metabolism
• Pyruvate metabolism
• 1,2-Dichloroethane degradation
• Propanoate metabolism
• 3-Chloroacrylic acid degradation
• Butanoate metabolism
• Limonene and pinene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
putrescine degradation I	2	1	1
putrescine degradation V	2	1	1
ethylene glycol degradation	2	1	1
ethanol degradation II	3	3	1
ethanol degradation IV	3	3	1
ethanol degradation III	3	2	1
hypotaurine degradation	3	2	1
putrescine degradation IV	3	2	1
histamine degradation	3	1	1
phytol degradation	4	3	1
fatty acid α-oxidation I (plants)	4	2	1
putrescine degradation III	4	2	1
L-tryptophan degradation X (mammalian, via tryptamine)	4	1	1
4-methylphenol degradation to protocatechuate	4	1	1
D-arabinose degradation II	4	1	1
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
mitochondrial NADPH production (yeast)	5	2	1
octane oxidation	5	2	1
dopamine degradation	5	1	1
3-methyl-branched fatty acid α-oxidation	6	3	1
alkane oxidation	6	1	1
4-hydroxymandelate degradation	6	1	1
noradrenaline and adrenaline degradation	13	4	2
serotonin degradation	7	3	1
superpathway of glycol metabolism and degradation	7	3	1
ceramide degradation by α-oxidation	7	2	1
2,4-xylenol degradation to protocatechuate	7	1	1
limonene degradation IV (anaerobic)	7	1	1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast)	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
superpathway of ornithine degradation	8	2	1
aromatic biogenic amine degradation (bacteria)	8	1	1
Entner-Doudoroff pathway II (non-phosphorylative)	9	5	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	5	1
toluene degradation III (aerobic) (via p-cresol)	11	3	1
superpathway of L-arginine and L-ornithine degradation	13	6	1
superpathway of aerobic toluene degradation	30	4	1
superpathway of aromatic compound degradation via 3-oxoadipate	35	8	1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate	42	8	1
superpathway of pentose and pentitol degradation	42	7	1