Experiment set3IT010 for Pontibacter actiniarum KMM 6156, DSM 19842

marine broth with D-Cycloserine 0.032 mg/ml

Group: stress
Media: marine_broth_2216 + D-Cycloserine (0.032 mg/ml)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.9 generations
By: Adam on 8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1739 C3

Specific Phenotypes

For 11 genes in this experiment

For stress D-Cycloserine in Pontibacter actiniarum KMM 6156, DSM 19842

For stress D-Cycloserine across organisms

SEED Subsystems

Subsystem	#Specific
Ton and Tol transport systems	2
Control of cell elongation - division cycle in Bacilli	1
DNA-binding regulatory proteins, strays	1
DNA Repair Base Excision	1
De Novo Purine Biosynthesis	1
Glycine Biosynthesis	1
Iron acquisition in Vibrio	1
Lipid A-Ara4N pathway ( Polymyxin resistance )	1
Oxidative stress	1
Potassium homeostasis	1
Siderophore Aerobactin	1
Teichuronic acid biosynthesis	1
Thioredoxin-disulfide reductase	1
Threonine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Glycine, serine and threonine metabolism
• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• C21-Steroid hormone metabolism
• Purine metabolism
• Bisphenol A degradation
• Starch and sucrose metabolism
• Polyketide sugar unit biosynthesis
• Aminosugars metabolism
• Linoleic acid metabolism
• Tetrachloroethene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Retinol metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-glucuronate biosynthesis (from UDP-glucose)	1	1	1
L-threonine degradation III (to methylglyoxal)	3	2	2
L-threonine degradation II	2	2	1
UDP-α-D-xylose biosynthesis	2	2	1
aminopropanol phosphate biosynthesis II	4	2	2
UDP-N-acetyl-α-D-galactosaminuronate biosynthesis	2	1	1
inosine-5'-phosphate biosynthesis II	5	5	1
UDP-2,3-diacetamido-2,3-dideoxy-α-D-mannuronate biosynthesis	5	1	1
inosine-5'-phosphate biosynthesis I	6	6	1
inosine-5'-phosphate biosynthesis III	6	5	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
superpathway of L-threonine metabolism	18	10	2
UDP-sugars interconversion	9	4	1
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
colanic acid building blocks biosynthesis	11	9	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	6	1
superpathway of purine nucleotides de novo biosynthesis II	26	23	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	42	1